Method for Inducing the Callus Formation of Radix Radix Radix Radix Radix Radix L. by Utilizing Young Tender Lateral Roots
A technology for callus and holly vulgaris is applied in the field of inducing the formation of callus of holly vulgaris by using young lateral roots of holly vulgaris, which can solve the problems of aging of mature tissues, low induction efficiency, planting and breeding of holly vulgaris, etc. Effects of protection, callus induction stabilization, and improved research efficiency
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[0058] (1) Select the holly holly seeds with good shape and not eaten by worms, and put them in 50mL sterilized centrifuge tubes;
[0059] (2) On the aseptic operating table, first add 40mL of 75% ethanol to sterilize the seeds of Ilex japonica for 10 minutes, and oscillate from time to time during this period;
[0060] (3) Pour off 75% ethanol, then use 40mL Clorox bleach (containing 6.25% sodium hypochlorite activity) to sterilize the holly seeds for 30 minutes, shaking from time to time;
[0061] (4) Throw away the Clorox bleach, and wash and sterilize the seeds 5 to 6 times with sterile water;
[0062] (5) Pour off the sterile water, and use sterilized tweezers on the sterile operating table to take out the seeds from the centrifuge tube and inoculate them directly into a petri dish (90×15mm) containing solid 1 / 2 MS medium (such as figure 1 shown);
[0063] Note: 1 / 2MS medium, 2.22g / L MS (containing Gamborg's vitamins) (Sigma); 2g / 100mL Sucrose (Caisson); 8g / L phytoblend...
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