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Method for Inducing the Callus Formation of Radix Radix Radix Radix Radix Radix L. by Utilizing Young Tender Lateral Roots

A technology for callus and holly vulgaris is applied in the field of inducing the formation of callus of holly vulgaris by using young lateral roots of holly vulgaris, which can solve the problems of aging of mature tissues, low induction efficiency, planting and breeding of holly vulgaris, etc. Effects of protection, callus induction stabilization, and improved research efficiency

Active Publication Date: 2018-11-09
SHENZHEN RES INST THE CHINESE UNIV OF HONG KONG
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, due to its long growth cycle, low seed setting rate and germination rate, and its vulnerability to insect damage, its reproductive ability is weak; its mature tissues are relatively aging, and the germinated seedlings are also prone to aging and lignification in the later stages of growth, which is not conducive to DNA. and RNA extraction, etc., and researchers cannot plant and breed Ilex japonica indoors, and have to pick a large number of new seeds every year for scientific research, which largely limits the application of molecular biotechnology in gene cloning and functional research of Ilex japonica
At present, there is no patent report about the tissue culture and callus induction of Ilex chinensis, only some Chinese periodicals have a small amount of reports about the induction of Ilex spp. callus, but the induction efficiency is low and unstable (3% to 80%). poor repeatability

Method used

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  • Method for Inducing the Callus Formation of Radix Radix Radix Radix Radix Radix L. by Utilizing Young Tender Lateral Roots
  • Method for Inducing the Callus Formation of Radix Radix Radix Radix Radix Radix L. by Utilizing Young Tender Lateral Roots
  • Method for Inducing the Callus Formation of Radix Radix Radix Radix Radix Radix L. by Utilizing Young Tender Lateral Roots

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example

[0058] (1) Select the holly holly seeds with good shape and not eaten by worms, and put them in 50mL sterilized centrifuge tubes;

[0059] (2) On the aseptic operating table, first add 40mL of 75% ethanol to sterilize the seeds of Ilex japonica for 10 minutes, and oscillate from time to time during this period;

[0060] (3) Pour off 75% ethanol, then use 40mL Clorox bleach (containing 6.25% sodium hypochlorite activity) to sterilize the holly seeds for 30 minutes, shaking from time to time;

[0061] (4) Throw away the Clorox bleach, and wash and sterilize the seeds 5 to 6 times with sterile water;

[0062] (5) Pour off the sterile water, and use sterilized tweezers on the sterile operating table to take out the seeds from the centrifuge tube and inoculate them directly into a petri dish (90×15mm) containing solid 1 / 2 MS medium (such as figure 1 shown);

[0063] Note: 1 / 2MS medium, 2.22g / L MS (containing Gamborg's vitamins) (Sigma); 2g / 100mL Sucrose (Caisson); 8g / L phytoblend...

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Abstract

The invention discloses a method for inducing ammopiptanthus callus formation by utilizing young and tender ammopiptanthus lateral roots. The method comprises the following steps: a, performing sterile seedling raising by utilizing ammopiptanthus seeds, and continuously inducing formation of lateral roots with root hair through a 1 / 2MS culture medium; and b, shearing young and tender hair lateral roots as explants, inducing callus formation on a CDB1 culture medium in the dark, and performing subculture and expanding propagation on the induced callus on a CDB6-2 culture medium under the light or in the dark. With the adoption of the method disclosed by the invention, the ammopiptanthus callus can be efficiently and stably obtained at low cost.

Description

technical field [0001] The invention relates to a method for inducing callus formation of holly holly by using young lateral roots of holly holly. Background technique [0002] Sand holly belongs to the genus Sand holly in the leguminous family. It is an evergreen shrub in a super-xerophytic desert, which is suitable for various extreme environments in the desert (drought, high temperature, low temperature, and wind erosion, etc.), and it is also an ancient relic species of the Tertiary period. , is an endangered species, so Ilex is a plant with good stress resistance and beneficial gene resources, and has the value of genetic evolution research on genes and plant biological traits. The predecessors only carried out a lot of research work on its natural protection, community distribution, biological characteristics and genetic background analysis. Until recently, some research reports were about the gene cloning and transcriptome sequencing analysis of Ilex chinensis. It is...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/005
Inventor 何军贤高志强
Owner SHENZHEN RES INST THE CHINESE UNIV OF HONG KONG
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