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A kind of fermentation medium and fermentation method of bacillus licheniformis

A technology of Bacillus licheniformis and fermentation method, which is applied in the field of microecological preparations and can solve the problems of high spore count, low cost, and low cost of culture medium

Active Publication Date: 2021-08-06
漳州大北农农牧科技有限公司 +1
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  • Claims
  • Application Information

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Problems solved by technology

[0008] However, the ratio of the culture medium required by different bacteria is different, resulting in the cost of the culture medium. However, the current culture medium has not yet been able to take into account the high number of viable fermentation bacteria, the high number of spores and the low cost.

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  • A kind of fermentation medium and fermentation method of bacillus licheniformis
  • A kind of fermentation medium and fermentation method of bacillus licheniformis

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Embodiment Construction

[0017] The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention.

[0018] The culture medium optimization of embodiment Bacillus licheniformis

[0019] 1) Determination of carbon source: Use 0.5% yeast extract powder, glucose, sucrose, maltose, and soluble starch to replace the yeast powder in the LB medium as the carbon source, keep the rest of the ingredients unchanged, prepare the medium for sterilization, and inoculate 2%, cultured at 37°C for 20 hours, and counted by the plate colony counting method. Each treatment was repeated three times, and the average value was taken. The number of viable bacteria and the number of spores were combined to determine the best carbon source. The results are shown in Table 1. The number of live bacteria and spores in the medium with yeast extract powder as carbon source are higher than those of other carbon sources, so yeast extract powder is the best carbon sou...

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Abstract

The invention belongs to the technical field of probiotics, and relates to a fermentation medium and a fermentation method of bacillus licheniformis. The culture medium provided by the present invention contains 0.75% yeast extract powder, 1-1.5% gelatin, 0.5% CaCl 2 . The medium optimizes the carbon source, nitrogen source, and inorganic salt in the basic medium, and the number of viable bacteria in the fermentation liquid of Bacillus licheniformis can reach 1×10 10 More than cfu / g.

Description

technical field [0001] The invention relates to a fermentation medium and a fermentation method of Bacillus licheniformis, belonging to the technical field of probiotics. Background technique [0002] Probiotics often use the finished medium products on the market during the fermentation process. Most of the finished products are ordinary medium or meet a certain type of bacteria. There is no way to provide the best formula for a certain strain of bacteria, resulting in a low number of viable bacteria. , high fermentation costs and other issues. [0003] Feng Guipeng, Yang Liyun, 2009, disclosed a Bacillus licheniformis fermentation medium, the optimal ratio is: corn steep liquor 0.45g, peptone 1.50g, bean cake powder soaking juice 1:50, glucose 1.50g. [0004] Liu Yang et al., 2012, disclosed that the culture medium of Bacillus licheniformis was optimized, and specifically disclosed that the order of significance of the effect of prime on the number of viable bacteria is: ...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/10
Inventor 陆黎明戴佳佳黄梅丹温灿权
Owner 漳州大北农农牧科技有限公司