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Composition for Improving Sialylation Level of Recombinant Human Type II Tumor Necrosis Factor Receptor-Antibody Fusion Protein

A composition and cofactor technology, applied in the biological field, can solve problems such as human beings that have not yet been regulated, and achieve the effects of being suitable for large-scale production, increasing the speed of enzyme reactions, and increasing the supply of substrates

Active Publication Date: 2019-08-20
HISUN BIOPHARMACEUTICAL CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at this stage, there is no effective measure to regulate the sialylation level of recombinant human tumor necrosis factor receptor type II-antibody fusion protein

Method used

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  • Composition for Improving Sialylation Level of Recombinant Human Type II Tumor Necrosis Factor Receptor-Antibody Fusion Protein
  • Composition for Improving Sialylation Level of Recombinant Human Type II Tumor Necrosis Factor Receptor-Antibody Fusion Protein
  • Composition for Improving Sialylation Level of Recombinant Human Type II Tumor Necrosis Factor Receptor-Antibody Fusion Protein

Examples

Experimental program
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Effect test

Embodiment 1

[0057] Example 1 In the batch culture process, it is applied to the commercial serum-free medium EX-CELL produced by SAFC Biosciences in the United States TM 302CHO composition for increasing the sialylation level of recombinant human type II tumor necrosis factor receptor-antibody fusion protein.

[0058] Eighteen types of compositions (Table 2) for increasing the sialylation level of recombinant human tumor necrosis factor receptor-antibody fusion protein are provided in this example, containing manganese chloride tetrahydrate, galactose, glucosamine, N- Acetylmannose, N-acetylglucosamine, uracil, dexamethasone, and hydrocortisone.

[0059] The preparation method of the composition in this example is: fully dissolve the prescribed amount of manganese chloride tetrahydrate, galactose, glucosamine, N-acetylmannose, N-acetylglucosamine and uracil in pyrogen-free ultrapure water Dissolve the prescribed amount of dexamethasone and hydrocortisone in 99% ethanol solution and add ...

Embodiment 2

[0069] Example 2 A composition for increasing the sialylation level of recombinant human tumor necrosis factor receptor II-antibody fusion protein applied to CDoptimal commercial basal medium developed by Life technology company in the United States during fed-batch culture.

[0070] The composition used in this embodiment for increasing the sialylation level of the recombinant human tumor necrosis factor receptor type II-antibody fusion protein contains:

[0071] Galactose: 30mmol / L

[0072] Glucosamine: 20mmol / L

[0073] N-acetylmannose: 20mmol / L

[0074] N-acetylglucosamine: 20mmol / L

[0075] Uracil: 20mmol / L

[0076] Manganese chloride tetrahydrate: 0.1mmol / L

[0077] Dexamethasone: 0.1mmol / L and

[0078] Hydrocortisone: 0.1mmol / L.

[0079] The preparation method of the composition in this example is: fully dissolve the prescribed amount of manganese chloride tetrahydrate, galactose, glucosamine, N-acetylmannose, N-acetylglucosamine and uracil in pyrogen-free ultrapu...

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Abstract

The invention relates to a composition used for improving the sialyl level of a recombinant human type II tumor necrosis factor receptor-antibody fusion protein. The composition comprises galactose (10-100mmol / L), glucosamine (1-50mmol / L), N-acetylmannosamine (1-50mmol / L), N-acetylglucosamine (1-50mmol / L), uracil (1-50mmol / L), tetrahydrate manganese chloride (0.01-1mmol / L), dexamethasone (0.01-1mmol / L) and hydrocortisone (0.01-1mmol / L). After the composition disclosed by the invention is added into a culture medium, the sialyl level of the recombinant human type II tumor necrosis factor receptor-antibody fusion protein produced by a large-scale animal cell culture technology can be obviously improved, so that the stability of the recombinant human type II tumor necrosis factor receptor-antibody fusion protein is improved.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular, the invention relates to a composition for increasing the sialylation level of recombinant human type II tumor necrosis factor receptor-antibody fusion protein. [0002] technical background [0003] Since the beginning of the 21st century, life science and biotechnology have become research and development hotspots in many countries, and the development level of the modern biotechnology industry is one of the important symbols of international competitiveness. Animal cell expression system and its large-scale culture technology are the most important production system and core technology in the biotechnology drug and biological product industry at this stage and even in the future for a long period of time. Its products include various antibodies, recombinant proteins, human, animal and poultry viruses Vaccines etc. Among them, recombinant human tumor necrosis factor receptor type II-...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/00C12R1/91
Inventor 邓献存王海彬范里邓全宇万宇翔潘洪辉徐胜缪仕伟白骅
Owner HISUN BIOPHARMACEUTICAL CO LTD
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