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A kind of quantum dot airgel microsensor for glucose, its preparation method and application of quantum dot airgel

A technology of microsensors and quantum dots, applied in chemical instruments and methods, laboratory containers, instruments, etc., can solve problems such as large errors, long analysis time, and expensive equipment, and achieve quantitative analysis and high-sensitivity quantitative analysis , Realize the effect of qualitative and rapid visual analysis

Active Publication Date: 2019-05-31
南京糖宁健康科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the titration method is simple to operate, but has a large error; the gas chromatography and high performance liquid phase methods have high sensitivity, but the analysis time is long and the equipment is expensive; the electrochemical analysis method is convenient and portable, but it is easily interfered by other substances

Method used

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  • A kind of quantum dot airgel microsensor for glucose, its preparation method and application of quantum dot airgel
  • A kind of quantum dot airgel microsensor for glucose, its preparation method and application of quantum dot airgel
  • A kind of quantum dot airgel microsensor for glucose, its preparation method and application of quantum dot airgel

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] like Figure 1-2 As shown, a quantum dot airgel microsensor for glucose includes a microfluidic chip body, and the microfluidic chip includes a chip layer 100, a chip matrix substrate layer 300, a detection chamber 201, a fluid inlet 101 and a fluid outlet 102, The fluid inlet 101 and the fluid outlet 102 are arranged on the chip layer 100, the detection chamber 201 is arranged between the chip layer 100 and the chip matrix substrate layer 300, and the two ends of the detection chamber 201 communicate with the fluid inlet 101 and the fluid outlet 102 respectively; the detection chamber 201 Quantum dot airgel for detecting glucose is installed inside; the material of the chip layer 100 is polydimethylsiloxane, UV resin or polymethyl methacrylate; the matrix substrate material is glass, silicon wafer or quartz; the chip layer 100 and the host substrate layer 300 are bonded by irreversible plasma oxidation or polymer material.

[0031] The quantum dot airgel preparation m...

Embodiment 2

[0040] Substantially the same as Example 1, the difference is: glucose solution is mixed with PBS damping fluid to make 0mM, 0.5mM, 1mM, 2mM, 4mM, 6mM, 8mM, 12mM, the glucose solution of 16mM concentration, and by injecting trace The sampler injects 1 μL of glucose solutions of various concentrations into the microfluidic chip. After 5 minutes, the microsensors are respectively fixed on the same position on the platform of the three-purpose ultraviolet analyzer, and the ultraviolet light is turned on in a dark environment and photographed with the camera equipment. ImageJ software is used for gray value analysis to measure the fluorescent gray value i of various concentrations of glucose, and the fluorescent gray value of the flow channel into pure PBS is i 0 , the concentration of glucose solution is C mM. Calculate 1-i / i 0 and log 10 The value of (C), and with 1-i / i 0 The average value of as the ordinate, log 10 The average value of (C) is used as the abscissa, and it is...

Embodiment 3

[0042] Basically the same as Example 1, the difference is: the quantum dot airgel preparation method for the quantum dot airgel microsensor of the above-mentioned glucose comprises the following steps:

[0043] (1) Synthesize cadmium chloride, sodium borohydride, glutathione, and sodium tellurite into CdTe quantum dots by using an aqueous phase synthesis method, and synthesize a quantum dot solution as the preparation object when the fluorescence intensity of the solution is maximum;

[0044] (2) After mixing isopropanol with the prepared quantum dot solution, the nanoparticles were separated by a centrifuge, and then dried in a vacuum dryer at 40° C. for 2 hours to obtain a dry CdTe quantum dot powder;

[0045] (3) Dissolve 0.8g of CdTe quantum dot powder in 30ml of PBS buffer solution, then take 0.4ml of the solution and add 10% glucose oxidase, and then inject the mixed solution into the detection chamber 201 with a micro injector;

[0046] (4) Put the microfluidic chip int...

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Abstract

The invention discloses a quantum-dot aerogel micro-sensor for glucose, a method for preparing quantum-dot aerogel and application of the quantum-dot aerogel micro-sensor. The quantum-dot aerogel micro-sensor comprises a micro-fluidic chip body. The micro-fluidic chip body comprises a chip layer, a chip matrix substrate layer, a detection cavity, a fluid inlet and a fluid outlet, the fluid inlet and the fluid outlet are formed in the chip layer, the detection cavity is arranged between the chip layer and the chip matrix substrate layer, and two ends of the detection cavity are respectively communicated with the fluid inlet and the fluid outlet; the quantum-dot aerogel for detecting the glucose is arranged in the detection cavity. The quantum-dot aerogel micro-sensor, the method and the application have the advantages that glucose solution with low concentration can be distinguished by the naked eyes of human bodies by the aid of the quantum-dot aerogel micro-sensor for the glucose, an obvious boundary is formed at the glucose solution with the concentration of 6 mM, flow channels of the quantum-dot aerogel micro-sensor appear dull when the concentration of the glucose solution is higher than 6 mM, and accordingly users can be reminded of excessively high concentration of the glucose at the moment; the glucose not only can be qualitatively quickly and visually analyzed by the quantum-dot aerogel micro-sensor, but also can be quantitatively analyzed by the quantum-dot aerogel micro-sensor in a high-sensitivity manner.

Description

technical field [0001] The invention relates to a microfluidic chip for detecting glucose, in particular to a quantum dot airgel microsensor for glucose, a preparation method of the quantum dot airgel and an application thereof. Background technique [0002] Quantum Dots (Quantum Dots) is a special nanoparticle, also known as semiconductor quantum dots, semiconductor nanoparticles or semiconductor nanocrystals. It has excellent spectral characteristics and photostability, and is an ideal fluorescent material. Compared with traditional fluorescent materials, quantum dots have the advantages of narrow fluorescence peak, good symmetry, high quantum yield, and good photobleaching resistance. [0003] Nowadays, with the continuous development of detection technology, people continue to improve the requirements for detection methods. People hope that the conditions required for detection will be reduced-the detection equipment is simple and easy to use, and detection can be carri...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64B01L3/00
CPCB01L3/5027B01L2200/027B01L2200/10B01L2300/12G01N21/6428G01N21/6447G01N2021/6432
Inventor 胡涛倪中华叶亦王振桑文
Owner 南京糖宁健康科技有限公司
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