Method for identifying Chinese agaricus sinodeliciosus

An identification method, a delicious technology, applied in the determination/inspection of microorganisms, biochemical equipment and methods, etc., can solve the problems of no quick method, complicated microscopic identification, low accuracy, etc., and achieve good application prospects and accurate identification High performance and accurate positioning effect

Inactive Publication Date: 2017-02-22
SHANGHAI ACAD OF AGRI SCI
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  • Application Information

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Problems solved by technology

However, microscopic identification is more complicated and the accuracy is not high
In the late 20th century, molecular sequences were gradually used in identification, but only as a reference
However, there has been no fast method for the identification of Agaricus species in China, especiall

Method used

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  • Method for identifying Chinese agaricus sinodeliciosus
  • Method for identifying Chinese agaricus sinodeliciosus
  • Method for identifying Chinese agaricus sinodeliciosus

Examples

Experimental program
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Example Embodiment

[0037] Example 1

[0038] Take some wild Chinese delicious mushrooms:

[0039] (1) External morphological identification

[0040] The external morphology of A. sinodeliciosus includes: 10-35cm large cap, 5-20cm thick stalk, pink and flesh-colored to brown to dark gills, and the fungus ring located on the upper part of the stalk And the whole fruit body is buried 45-55 cm underground with trees growing around;

[0041] (2) Anatomy identification

[0042] Cut the Chinese delicacy mushroom from the middle of the cap and stalk, let it stand for 2 minutes, the section changes from white to pink;

[0043] (3) Identification of chemical reagents

[0044] Take the pre-prepared 5% KOH solution, drop it on the surface of the cap of the fresh Chinese delicious mushroom fruit body, let it stand for 5 minutes, the titration place of the KOH solution will turn light yellow;

[0045] (4) Molecular sequence identification

[0046] ①DNA extraction: extract total DNA and adjust the concentration to 100ng / μL...

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Abstract

The invention relates to a method for identifying Chinese agaricus sinodeliciosus. The method includes the steps of (1) external form identifying; (2) microstructure identifying; (3) chemical reagent detection; (4) molecular sequence identifying. According to the method, the A. sinodeliciosus is identified with the method of combining identifying of the external form characteristic, changes in the color of an anatomical structure, a chemical reagent and the molecular sequence, and the method has the advantages of being high in identification accuracy and accurate in positioning; the method is representative in identification of Chinese agaricus sp. classes, and has good application prospects.

Description

technical field [0001] The invention belongs to the field of mushroom identification, in particular to an identification method of Chinese delicious mushroom (A. sinodeliciosus). Background technique [0002] The identification of mushrooms in my country has existed since the beginning of the 20th century, but it has always been relatively primitive, and the identification is not accurate, and in many cases it cannot be located at the species level. With the application of microscopy in mushroom identification, microstructure observation has been applied in the identification of mushrooms. However, microscopic identification is more complicated and the accuracy is not high. In the late 20th century, molecular sequences were gradually used in identification, but only as a reference. There are as many as thousands of species in the genus Agaricus, and the species in the genus Agaricus in China accounts for a large part of the world. However, there has been no fast method fo...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12Q1/04
CPCC12Q1/6895C12Q1/04C12Q1/6869C12Q2531/113
Inventor 李传华曲明清李正鹏宋晓霞奚莉萍汪虹陈明杰李文张劲松鲍大鹏黄建春沈学香
Owner SHANGHAI ACAD OF AGRI SCI
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