Applications of vitamin E in preparation of drugs for prevention and treatment of ischemia-reperfusion-induced arrhythmia

A technique for ischemia-reperfusion and arrhythmia, applied in drug combination, food science, pharmaceutical formula, etc., can solve problems such as arrhythmia, and achieve the effect of reducing risk and reducing QT interval prolongation

Inactive Publication Date: 2017-03-01
SOUTH CHINA UNIV OF TECH
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no effective drug for the treatment of ischemia-reperfusion-induc

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Applications of vitamin E in preparation of drugs for prevention and treatment of ischemia-reperfusion-induced arrhythmia
  • Applications of vitamin E in preparation of drugs for prevention and treatment of ischemia-reperfusion-induced arrhythmia
  • Applications of vitamin E in preparation of drugs for prevention and treatment of ischemia-reperfusion-induced arrhythmia

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1 Vitamin E anti-isolated guinea pig heart ischemia-reperfusion caused QT interval prolongation effect test

[0020] Methods: Before the operation, the surgical instruments were prepared and soaked in 75% (v / v) alcohol overnight. After preparing the K-H solution, take an appropriate amount and pour it into a glass petri dish, and place the glass petri dish on ice (at about 4°C) for oxygenation. Give 0.5mL 1% (m / v) heparin by intraperitoneal injection, and after 20-30min, intraperitoneally inject 5% (m / v) pentobarbital sodium (0.2mL / 100g) for anesthesia, after deep anesthesia, guinea pigs ( Guinea pig) (adult ordinary hartly guinea pig, purchased from the Experimental Animal Center of Guangzhou University of Traditional Chinese Medicine) was placed on the operating table with its back, and the limbs were fixed with medical tape. The xiphoid process was quickly opened to expose the heart and blood vessels. The root of the heart was clamped with tweezers, the supe...

Embodiment 2

[0027] Embodiment 2 Vitamin E reduces the effect of ischemia-reperfusion on I Kr Inhibition test of current (hERG-related current)

[0028] Cardiomyocyte separation method: take out the heart part, see Example 1. After taking out the heart, find the aorta, put the aorta on the bottom of the cannula, pay attention not to insert the aortic valve, and tie it tightly with sutures. Perfuse with calcium-free Tyrode's solution for 4 to 5 minutes to pump out the blood to completely discharge the blood from the heart. Then use 95% (v / v) O 2 +5%(v / v)CO 2 Oxygen-saturated 15mL 0.4mg / mL type II collagenase (Worthington) enzyme solution was perfused circularly. Constantly check the hardness of the heart. After the heart becomes soft, drop a small amount of 0.4mg / mL type II collagenase enzyme solution and observe whether there are cardiomyocytes under an inverted microscope. Cut the heart into pieces in mg / mL type II collagenase (worthington), and blow gently in a 37°C water bath to acc...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The present invention discloses applications of vitamin E in preparation of drugs for prevention and treatment of ischemia-reperfusion-induced arrhythmia, and belongs to the pharmaceutical field. According to the present invention, the results show that vitamin E has the anti-ischemia-reperfusion-induced arrhythmia effect; with the application of the vitamin E in preparation of drugs for prevention and treatment of heart ischemia reperfusion QT interval prolongation and associated arrhythmia, the action target point of the drug is clear, and the drug is the novel ischemia reperfusion QT interval prolongation prevention and treatment drug having the known molecular structure; and through the in vitro heart ischemia reperfusion model, the results prove that the drug can reduce the QT interval prolongation caused by ischemia-reperfusion so as to reduce the related arrhythmia risk.

Description

technical field [0001] The invention relates to a new application of vitamin E in the field of pharmacy, in particular to the application of vitamin E in the preparation of drugs for preventing and / or treating arrhythmia induced by ischemia-reperfusion. Background technique [0002] In recent years, the incidence of coronary heart disease has been increasing year by year and tends to be younger. Acute myocardial infarction (AMI) is the most serious type of coronary heart disease. Nearly one million patients die of acute myocardial infarction in my country every year. Therefore, the disease is It is regarded as "the first killer of human health". The most effective treatment for acute myocardial infarction is to achieve complete reperfusion of myocardial tissue on the basis of coronary artery opening, that is, to achieve myocardial microcirculation reperfusion. Currently commonly used treatment methods include thrombolysis and coronary interventional surgery. However, thromb...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61K31/355A61P9/06A61P3/10A61P9/12A23L33/15
Inventor 谭文殷春霞陈耀旭尚瑾玲杨莹莹
Owner SOUTH CHINA UNIV OF TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap