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A kind of rapid propagation method of seedlings using the bulbs of Polygonatum chinensis as explants

A technology of chicken head polygonatum and explants, applied in botany equipment and methods, planting substrates, gardening methods, etc., can solve the problems of unseen artificial rapid breeding of chicken head polygonatum, shorten the cultivation time and increase the cultivation temperature , the effect of fast reproduction

Inactive Publication Date: 2018-03-13
NINGXIA ACADEMY OF AGRI & FORESTRY SCI
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  • Abstract
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  • Claims
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Problems solved by technology

[0004] However, there is no report on the artificial rapid breeding of Polygonatum japonicus at present

Method used

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  • A kind of rapid propagation method of seedlings using the bulbs of Polygonatum chinensis as explants
  • A kind of rapid propagation method of seedlings using the bulbs of Polygonatum chinensis as explants
  • A kind of rapid propagation method of seedlings using the bulbs of Polygonatum chinensis as explants

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Embodiment 1

[0025] A method for rapid propagation of seedlings using the bulbs of Polygonatum polygonatum produced in Liupanshan, Ningxia as explants, comprising:

[0026] 1. Selection and disinfection of explants: select fresh and mature bulbs of Polygonatum Polygonatum produced in Liupanshan, Ningxia in autumn (late August to early September), remove the residual old roots at the base of the bulbs, and rinse them with tap water for 30 minutes. Then drop into the sodium hypochlorite solution that is 2% by volume and soak for 20min, take it out and rinse it with clear water for 4-5 times, then transfer to the ultra-clean workbench, and carry out secondary disinfection on the ultra-clean workbench, specifically first use 75 Soak in % ethanol for 20s, quickly rinse with sterile water 3-4 times, then transfer to 0.1% mercuric acid solution (containing 0.1mL Tween 20 per liter) and soak for 8min, take it out and rinse with sterile water 4-5 times ;

[0027] 2. Material processing: use steril...

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Abstract

The invention discloses a method using Polygonatum sibiricum Redoute bulbs as explants to fast breed seedlings. The method includes: selecting and sterilizing the explants, processing, performing initiation culture, differentiating the bulbs, proliferating, inducing bulb tillering seedlings, performing rooting cultivation on test-tube seedlings, performing greenhouse seedling hardening, transplanting and the like. The method has the advantages that a fast breeding system of Polygonatum sibiricum Redoute is built, the bulb proliferation coefficient reaches 6.7, the test-tube seedling rooting rate reaches 98%, and the survival rate of greenhouse transplanting reaches more than 90%; the method is an efficient and fast breeding technology, large-scale seedling production can be achieved in a short time, and the problem of Polygonatum sibiricum Redoute seedling resource shortage is solved to a certain degree.

Description

technical field [0001] The invention relates to a method for rapid propagation of seedlings by using the bulbs of Polygonatum polygonatum as explants. Background technique [0002] Polygonatum sibiricum Delar.ex Redoute, also known as Chicken Head Polygonatum and Chicken Head Ginseng, belongs to the perennial herb of Liliaceae (Liliaceae) Polygonatum (Polygonatum). It is mainly distributed in Liupanshan area of ​​Ningxia. It has a medicinal history of more than 2,000 years in my country. Polygonatum is used as medicine with dried rhizomes. It is sweet in taste and flat in nature. It is used for deficiency of stomach yin, deficiency of spleen and stomach qi, fatigue, fatigue, dry mouth and lack of food, dry cough due to lung deficiency, deficiency of essence and blood, consumptive cough and hemoptysis, soreness of waist and knees, premature graying of beard and hair, and internal heat. Diabetes and other effects (Pharmacopoeia of the People's Republic of China 2010). [0003...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00A01G31/00A01G24/28A01G24/25A01G24/15
CPCA01G24/00A01G31/00A01H4/001A01H4/008
Inventor 郭生虎朱永兴陈虞超关雅静石磊张农
Owner NINGXIA ACADEMY OF AGRI & FORESTRY SCI
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