Identification method of female parent source of purple scallop and bay scallop and their backcross offspring

Abstract

The invention relates to a method for identifying argopectenpurpuratus, argopectenirradians and a female parent source of a backcross generation of the argopectenpurpuratus and the argopectenirradians. A mark primer mtDNA-Z designed based on the difference of DNA sequences of mitochondria consists of a left end primer sequence mtDNA-Z-F: 5'-TATGAGGTGTCCCCCAAGTC-3' and a right end primer sequence mtDNA-Z-R: 5'-ACTGGCAGACAAACAAATCGT3'; the DNAs of the extracted argopectenpurpuratus, the extracted argopectenirradians and a backcross scallop are used as templates, and primer pairs are used for carrying out PCR amplification on the templates; if a stripe with PCR specificity appears at the near 460 bp position, a female parent mitochondrial gene source is the argopectenpurpuratus; if the stripe with the PCR specificity does not appear at the near 460 bp position, the female parent mitochondrial gene source is the argopectenirradians. The method eliminates the influence of the DNA of a nuclear genome, so that the DNA of mitochondria is not needed to be independently extracted for identification; therefore, the argopectenpurpuratus, the argopectenirradians and the parent source of the backcross generation of the argopectenpurpuratus and the argopectenirradians can be quickly and efficiently identified; the method is simple, convenient and short in period, can realize batch identification, and supplies a technical support and guarantee in order to guarantee success of seed cultivation and confirm the pedigree of the backcross generation.

Description

technical field [0001] The invention belongs to the progeny identification technology of scallop hybrid breeding, and in particular relates to a method for identifying the source of female parents of purple scallops, bay scallops and their backcross offspring. Background technique [0002] Gulf scallop (Argopectenirradians) has been increasing year by year since it was introduced from the United States in 1982, and has become one of the most important cultured shellfish in China. [0003] Purple scallop (Argopecten purpuratus) is an excellent economic shellfish on the Pacific coast of South America. It is medium in size and widely cultivated in Chile and Peru, making Chile the second largest scallop farming country after China. In order to improve the germplasm of bay scallops and increase the economic output of scallop farming, Wang Chunde and others introduced purple scallops from Peru and successfully crossed them with bay scallops in 2008, and bred two generations of hyb...

AI Technical Summary

Problems solved by technology

However, purple scallops and bay scallops are both hermaphroditic animals, and both scallops release sperm and eggs at the same time during breeding. There is a problem of sperm or egg contamination in breeding practice. In order to ensure the smooth progress of the breeding process and confirm the pedigree of the offspring , it is often necessary to determine whether a certain offspring is a true backcross offspring and its maternal origin, so it is necessary to establish a method to quickly identify its parental origin, especially the maternal origin

[0004] Although the identification method using molecular markers (such as microsatellite markers) from the nuclear genome can be used to identify whether the backcross offspring is a true hybrid, it cannot distinguish the parental origin of the backcross offspring, which leads to confusion in the pedigree of the backcross offspring and makes it difficult to identify excellent lines Screening and other issues, and in the backcrossing test, it was found that the traits of the first generation of backcrossing scallops were different, and some even completely expressed the traits of the mother, so the rapid identification of the female parent of the backcrossing scallop source is very necessary

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