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Use of biomarkers for preparing diagnostic reagents for cystitis glandularis

A diagnostic reagent and cystitis technology, applied in the field of diagnostic reagents, can solve the problems of lack of characteristic clinical manifestations of cystitis glandularis

Inactive Publication Date: 2019-05-14
北京开景基因技术有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the lack of characteristic clinical manifestations of cystitis glandularis and the defects and deficiencies of the existing inspection methods, it is necessary to develop relevant diagnostic reagents to diagnose and classify cystitis glandularis

Method used

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  • Use of biomarkers for preparing diagnostic reagents for cystitis glandularis
  • Use of biomarkers for preparing diagnostic reagents for cystitis glandularis
  • Use of biomarkers for preparing diagnostic reagents for cystitis glandularis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1 Diagnostic reagent based on blood circulation miRNA molecule

[0034] Part 1: Screening Phase

[0035] 1. Experimental materials and experimental methods

[0036] 1. Plasma samples: 23 subjects, aged 45-55 years, with an average of 51.3 years; 12 males and 11 females. Grouping confirmed by cystoscopy: chronic inflammation group (6 cases of chronic inflammation type, 3M / 3F), follicular edema group (5 cases of follicular edema type, 3M / 2F), papilloma group (5 cases of papilloma type, 2M / 3F), intestinal adenomatous group (7 cases of intestinal adenomatous type, 4M / 3F). All cases were newly diagnosed and had no history of drug treatment. Another 5 cases with normal bladder served as the control group. There was no significant difference in the age of the subjects in each group. All patients and those with normal bladder had normal heart, lung, liver, kidney and hematopoietic functions. Normal bladder refers to healthy volunteers with no abnormal prolifer...

Embodiment 2

[0060] Example 2 Diagnostic reagents based on plasma small molecule metabolites

[0061] Part 1: Screening Phase

[0062] 1. Experimental materials and experimental methods

[0063] 1. Plasma samples: 23 subjects, aged 45-55 years, with an average of 51.3 years; 12 males and 11 females. Grouping confirmed by cystoscopy: chronic inflammation group (6 cases of chronic inflammation type, 3M / 3F), follicular edema group (5 cases of follicular edema type, 3M / 2F), papilloma group (5 cases of papilloma type, 2M / 3F), intestinal adenomatous group (7 cases of intestinal adenomatous type, 4M / 3F). All cases were newly diagnosed and had no history of drug treatment. Another 5 cases with normal bladder served as the control group. There was no significant difference in the age of the subjects in each group. All patients and those with normal bladder had normal heart, lung, liver, kidney and hematopoietic functions. Normal bladder refers to healthy volunteers with no abnormal prolifer...

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Abstract

The invention discloses application of biomarkers to preparation of a glandular cystitis diagnostic reagent. Glandular cystitis comprises a chronic inflammation type, a follicular edema type, a papilloma type and an intestine adenoma type. The biomarkers comprise miR-372-3p indicated by SEQ ID NO.1, miR-383-3p indicated by SEQ ID NO.2 and miR-377-3p indicated by SEQ ID NO.3. When the miR-372-3p, the miR-383-3p and the miR-377-3p are combined to be used for diagnosing and distinguishing glandular cystitis patients with persons with normal bladders, ROC area under the cure (AUC) is 0.964, and the flexibility and specificity are 96.2% and 95.6% respectively. The glandular cystitis types can be diagnosed and distinguished without the interventional procedures by using the diagnostic reagent, only small blood plasmas of the patients are needed, taboos are avoided during checking, and defects of cystoscopy are overcome.

Description

technical field [0001] The invention belongs to the field of diagnostic reagents, and in particular relates to the use of biomarkers for preparing diagnostic typing reagents for cystitis glandularis. Background technique [0002] Cystitis glandularis (CG) is a relatively rare non-neoplastic inflammatory lesion. It is a lesion in which epithelial hyperplasia and metaplasia coexist. Cracks form branched and annular lumens, and glandular metaplasia appears in the center to form glandular structures. At the same time, lymphocytes and plasma cells infiltrate, so it is called glandular cystitis. To put it simply, cystitis glandularis is a kind of abnormal proliferation and metaplasia of mucous glands in bladder mucosa and submucosa, its incidence rate is increasing day by day, and it is closely related to bladder cancer (Yang Anqing et al. Expression in cystitis and its clinical significance, 2016). Whether cystitis glandularis is a precancerous lesion is currently controversial...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6883
CPCC12Q1/6883C12Q2600/112C12Q2600/178
Inventor 不公告发明人
Owner 北京开景基因技术有限公司