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A method of preparing gel used for skin rejuvenating

A technology for repairing skin and gel, applied in medical science, bandages, etc., can solve the problems of lack, poor recovery of hair follicles, accompanied by a large number of scars, etc., to improve exercise ability, speed, and increase superoxide dismutase activity. Effect

Inactive Publication Date: 2017-04-19
NINGBO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is still a lack of effective and effective treatment methods for deep second-degree and third-degree burns in the world, and various complications caused by large-scale burns can lead to a mortality rate as high as 20%.
Moreover, the biggest problem in the repair of damaged skin is: although the existing methods can speed up the healing of the wound surface and greatly reduce the death caused by infection and other complications, the regrown skin has a serious deficiency in function, such as Extremely poor hair follicle recovery with extensive scarring

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Embodiment one: a kind of preparation method for the gel of repairing skin, comprises the following specific steps:

[0016] (1) Dissolving hyaluronic acid with an average molecular weight of 800,000 Daltons in water to obtain an aqueous solution of hyaluronic acid with a mass concentration of 10%;

[0017] (2) Dissolving polysaccharides from versicolor versicolor in water to obtain an aqueous solution of polysaccharides with a mass concentration of 2%;

[0018] (3) Mix the hyaluronic acid aqueous solution and the polysaccharide aqueous solution at a volume ratio of 10:1 to obtain a mixed aqueous solution, then add an aqueous sodium hydroxide solution with a concentration of 0.2 mol / L to the mixed aqueous solution, and stir evenly to make the mixed aqueous solution When the pH value reaches 9, vinyl sulfone is added to the mixed aqueous solution, wherein: the mass ratio of the mixed aqueous solution to vinyl sulfone is 20:1 to form a uniform viscous liquid;

[0019] (4...

Embodiment 2

[0021] Embodiment two: a kind of preparation method for the gel of repairing skin, comprises the following specific steps:

[0022] (1) Dissolving hyaluronic acid with an average molecular weight of 1,800,000 Daltons in water to obtain an aqueous solution of hyaluronic acid with a mass concentration of 1%;

[0023] (2) Dissolving the aloe polysaccharide in water to obtain an aqueous polysaccharide solution with a mass concentration of 10%;

[0024] (3) Mix the hyaluronic acid aqueous solution and the polysaccharide aqueous solution at a volume ratio of 90:1 to obtain a mixed aqueous solution, then add an aqueous sodium hydroxide solution with a concentration of 0.2 mol / L to the mixed aqueous solution, and stir evenly to make the mixed aqueous solution When the pH value reaches 11, add dimethyl sulfoxide to the mixed aqueous solution, wherein: the mass ratio of the mixed aqueous solution to dimethyl sulfoxide is 5:1 to form a uniform viscous liquid;

[0025] (4) Coat the visco...

Embodiment 3

[0027] Embodiment three: a kind of preparation method for the gel of repairing skin, comprises the following specific steps:

[0028] (1) Dissolving hyaluronic acid with an average molecular weight of 1,400,000 Daltons in water to obtain an aqueous hyaluronic acid solution with a mass concentration of 6%;

[0029] (2) Dissolving Bletilla striata polysaccharide in water to obtain an aqueous polysaccharide solution with a mass concentration of 20%;

[0030] (3) Mix the hyaluronic acid aqueous solution and the polysaccharide aqueous solution at a volume ratio of 50:1 to obtain a mixed aqueous solution, then add an aqueous sodium hydroxide solution with a concentration of 0.2 mol / L to the mixed aqueous solution, and stir evenly to make the mixed aqueous solution When the pH value reaches 8, epichlorohydrin is added to the mixed aqueous solution, wherein: the mass ratio of the mixed aqueous solution to epichlorohydrin is 10:1 to form a uniform viscous liquid;

[0031] (4) Coat the...

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PUM

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Abstract

A method of preparing gel used for skin rejuvenating is disclosed. The method is characterized in that a hyaluronic acid aqueous solution and a polysaccharide aqueous solution are firstly prepared and then mixed according to a ratio to obtain a mixed aqueous solution, then an aqueous sodium hydroxide solution is added to the mixed aqueous solution to allow the pH value of the mixed aqueous solution to be 8-11, then a crosslinking agent is added into the mixed aqueous solution to form a uniform viscous fluid, a medical non-woven fabric is coated with the viscous fluid and is allowed to stand at 20-40 DEG C for 2-5 h to form gel, the gel is soaked in sterile water to wash off the excessive crosslinking agent, then the gel is soaked into alcohol having a volume concentration of 75% and disinfected, the gel is then soaked with a PBS solution, taken out and stored at 4 DEG C, and the gel is kept for later use; and the method is advantageous in that by using the gel prepared by the method for repairing human or animal full-thickness skin, scar formation can be reduced to the utmost and biological functions of the skin can be restored.

Description

technical field [0001] The invention relates to the preparation of the gel used in the medical field, in particular to a preparation method of the gel used for repairing skin. Background technique [0002] Skin is the physical barrier between the human body and the external environment, and is one of the largest and most important tissues and organs of the human body. Its basic function is to prevent the loss of water, electrolytes and nutrients in the body, and prevent the intrusion of harmful or unwanted substances from the outside. In addition, the skin also has physiological functions such as immunity, sensation, metabolism, and temperature regulation, so that the body is in a stable internal environment and can better adapt to changes in the external environment, which is very important for the health of the body. [0003] However, skin damage occurs from time to time, such as burns, which are a common serious disease in the world. About 50 million people have burns ev...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L26/00
CPCA61L26/0066A61L26/0023A61L26/0061A61L2300/232A61L2300/236A61L2300/404A61L2300/408A61L2300/412C08L5/08C08L5/00
Inventor 竺亚斌
Owner NINGBO UNIV
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