Myocardial infarction treating cell preparation, preparation method and applications thereof
A technology of myocardial infarction and cell preparation, which is applied in the field of biomedicine, can solve the problems of incomplete cure of myocardial infarction, achieve the effect of improving ischemic myocardial fibrosis, improving pathological remodeling, and reducing the incidence rate
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[0040] (1) Preparation of CTs
[0041]1. SD female rats (250-300g) (Guangdong Provincial Medical Experimental Animal Center) were killed by pulling the neck after ether anesthesia, soaked in 70 (v / v)% ethanol for 5 minutes, and quickly transferred to a biological safety cabinet.
[0042] 2. Under sterile conditions, quickly cut open the chest cavity and take out the heart, put the heart into sterile PBS solution (0.01M, pH7.4) and wash it 3 times to remove the residual blood in the heart, and cut the heart tissue in a sterile plate (1-2mm 3 ), then transfer the heart tissue block to a 50ml sterile centrifuge tube, add 37°C preheated digestive enzyme solution [0.05 (w / v)% collagenase + 0.1 (w / v)% trypsin, The same below] 2.5ml, after sealing the tube cap, digest it in a water-bath shaker (300rpm) at 37°C for 10min, then let it stand in a biological safety cabinet for 2min, and discard the supernatant suspension.
[0043] 3. In the biosafety cabinet, add 2.5ml of digestive enz...
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