Myocardial infarction treating cell preparation, preparation method and applications thereof

A technology of myocardial infarction and cell preparation, which is applied in the field of biomedicine, can solve the problems of incomplete cure of myocardial infarction, achieve the effect of improving ischemic myocardial fibrosis, improving pathological remodeling, and reducing the incidence rate

Inactive Publication Date: 2017-05-03
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, its curative effect is only reflected in reducing the infarct size, increasing the ejection fraction, improving cardiac function, and improving pathological left ventricular remodeling, but the above curative effects cannot completely cure myocardial infarction.

Method used

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  • Myocardial infarction treating cell preparation, preparation method and applications thereof
  • Myocardial infarction treating cell preparation, preparation method and applications thereof
  • Myocardial infarction treating cell preparation, preparation method and applications thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0040] (1) Preparation of CTs

[0041]1. SD female rats (250-300g) (Guangdong Provincial Medical Experimental Animal Center) were killed by pulling the neck after ether anesthesia, soaked in 70 (v / v)% ethanol for 5 minutes, and quickly transferred to a biological safety cabinet.

[0042] 2. Under sterile conditions, quickly cut open the chest cavity and take out the heart, put the heart into sterile PBS solution (0.01M, pH7.4) and wash it 3 times to remove the residual blood in the heart, and cut the heart tissue in a sterile plate (1-2mm 3 ), then transfer the heart tissue block to a 50ml sterile centrifuge tube, add 37°C preheated digestive enzyme solution [0.05 (w / v)% collagenase + 0.1 (w / v)% trypsin, The same below] 2.5ml, after sealing the tube cap, digest it in a water-bath shaker (300rpm) at 37°C for 10min, then let it stand in a biological safety cabinet for 2min, and discard the supernatant suspension.

[0043] 3. In the biosafety cabinet, add 2.5ml of digestive enz...

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Abstract

The present invention discloses a myocardial infarction treating cell preparation, a preparation method and applications thereof, wherein the main components in the cell preparation are CTs and BMSCs. According to the present invention, with the direct intracardiac injection or the interventional intracardiac injection of the cell preparation, by performing cell preparation transplantation on the ischemic area and ischemic margin area of the heart of acute myocardial infarction, the severely damaged cell network formed from CTs in the ischemic myocardium ischemic area can be effectively repaired and regenerated so as to repair the cell structure microenvironment beneficial for the regeneration of the infarcted myocardium, such that the regeneration of the infarcted myocardium is easily achieved; and after the transplantation treatment on the acute myocardial infarction, the effects in the fields of infarction area reducing, myocardial infarction heart cardiac function improving, ischemic myocardium fibrosis improving, ischemic area angiogenesis and marginal area angiogenesis promotion, infarcted heart pathological reconstruction improving and after-myocardial-infarction heart failure incidence reducing are superior to the effect of the current commonly-used separate BMSCs treatment.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to a cell preparation for treating myocardial infarction, a preparation method and application. Background technique [0002] At present, regardless of the basic and clinical aspects, although the treatment of myocardial infarction has made great progress in recent decades, there is still no drug or therapy that can cure myocardial infarction. Cell therapy is considered to be one of the most promising treatments for myocardial infarction. Both basic and clinical studies have proved that the cell therapy of bone marrow mesenchymal stem cells (BMSCs) to infarcted myocardium has been proved to be feasible and safe. But its efficacy is only reflected in reducing the infarct size, increasing ejection fraction, improving cardiac function, and improving pathological left ventricular remodeling, but the above effects cannot completely cure myocardial infarction. Therefore, the curre...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/28A61P9/10
Inventor 蔡冬青廖肇福赵宝寅沈晓涛郑馨陈夷林李艳梅许甘霖齐绪峰徐莎莎
Owner JINAN UNIVERSITY
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