Photo-sensitive medicine sensitive to weak light and preparation method of photo-sensitive medicine
A photosensitive drug, weak light technology, applied in the direction of drug combination, pharmaceutical formulation, medical preparations with non-active ingredients, etc. High yield, fast speed, and the effect of improving hypoxic environment
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Embodiment 1
[0043] The concentration of RB contained in the configuration is 0.01mg·mL under the condition of avoiding light -1 3.5 mL of Hb-RB solution, with 70 μL of 1 mg·mL -1 After the DMSO solution of 9,10-Diphenylanthracene (DPA) was mixed evenly, it was placed in a closed quartz cuvette. Place the cuvette at an intensity of about 50μW / cm 2 Under the weak light irradiation, the absorbance of the system in the range of 350-410nm was measured by a UV-visible spectrophotometer, measured every 5 minutes of irradiation, and the change of the absorbance at 380nm was compared.
[0044] DPA is a commonly used chemical trapping agent, which can quickly react with singlet oxygen to form a stable inner oxide, and the content of singlet oxygen can be measured by measuring the decrease of its absorbance at 380nm. like figure 1 As shown, it can be seen that with the prolongation of the irradiation time, the absorbance of the system at 380nm gradually weakens, indicating that the generation of ...
Embodiment 2
[0046] Prepare 0.01mg·mL under dark conditions -1 RB solution and the concentration of RB contained in it is 0.01mg·mL -1 Each 1mL of Hb-RB solution was mixed with 20μL of 1mg·mL -1 After the DMSO solution of DPA was mixed evenly, it was placed in a closed micro-quartz cuvette. Use a wavelength of 550nm and a power of 2mW / cm 2 Irradiate the cuvette with the exciting light, measure the absorbance of the system in the range of 350-410nm every minute, and compare the change of the absorbance at 380nm. like figure 2 As shown, it can be seen that with the prolongation of the irradiation time, the absorbance at 380 nm in the two systems decreases gradually, and the decrease is more obvious in the Hb-RB system. This phenomenon shows that under the same conditions, the Hb-RB system will generate more singlet oxygen.
Embodiment 3
[0048] Prepare the RB concentration at 0.01mg·mL under dark conditions -1 3.5 mL of Hb-RB solution, with 70 μL of 1 mg·mL - 1 After the DMSO solution of DPA was mixed evenly, it was placed in a quartz cuvette. Nitrogen gas was first introduced to remove the molecular oxygen in the system, and then the system was exposed to indoor visible light conditions (the light intensity was 1.5mW / cm 2 ), feed oxygen, and measure the absorbance of the system in the range of 350-410nm every 5s. like image 3 As shown, it can be seen that the absorbance of the solution at 380nm has a significant decrease, that is, the system generates a large amount of singlet oxygen during the process of introducing oxygen, which further indicates that Hb-RB is very sensitive to excitation light, and there are molecules in the system Under the condition of oxygen, it can be excited by ordinary natural light, avoiding the use of high-energy lasers.
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