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Method for Simultaneously Separating Quercetin-3-o-gentiobioside and Kaempferol-3-o-gentiobioside from Asparagus Leaf

A gentiobiglycoside and simultaneous separation technology is applied in chemical instruments and methods, sugar derivatives, sugar derivatives, etc., and can solve the problems of low content of quercetin and kaempferol, low purity, complicated process and the like, Achieve the effect of low dissolution rate, high separation efficiency and easy operation

Active Publication Date: 2019-01-08
CHENGDU PUSH BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the process of this technical solution is complicated, the cost is high, and there are too many types of solvents involved, and the content of quercetin and kaempferol in the extracted flavonoids is low, and the purity is not high; and the quercetin and chrysin involved , apigenin, kaempferol and luteolin are flavone aglycones, but no flavone glycosides are mentioned. Because the polarity of flavone aglycones and flavone glycosides is very different, this technical solution is not suitable for Extraction and separation of quercetin-3-0-gentiobioside and kaempferol-3-0-gentiobioside

Method used

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  • Method for Simultaneously Separating Quercetin-3-o-gentiobioside and Kaempferol-3-o-gentiobioside from Asparagus Leaf
  • Method for Simultaneously Separating Quercetin-3-o-gentiobioside and Kaempferol-3-o-gentiobioside from Asparagus Leaf
  • Method for Simultaneously Separating Quercetin-3-o-gentiobioside and Kaempferol-3-o-gentiobioside from Asparagus Leaf

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Such as figure 1 , figure 2 Shown: the method for simultaneously isolating quercetin-3-0-gentiobioside and kaempferol-3-0-gentiobioside from leaves of Asparagus japonica, comprising the following process steps:

[0044] (1) extract

[0045] Weigh 1Kg of Asparagus leaf medicinal material, crush it into a coarse powder of 1-4mm, then add 6L of 70% ethanol solution, reflux extraction for 3 times, 2 hours each time, filter, and combine the filtrates, a total of 30L, is the extract Ⅰ;

[0046] (2) concentrated

[0047] After recovering the ethanol from the extract solution I obtained in step (1), concentrate it to a small volume, and the resulting concentrated solution is 5 L, which is the extract solution II;

[0048] (3) Macroporous resin column enrichment and elution

[0049] Adsorb the extract II obtained in step (2) with a macroporous resin, wash the resin with 10L of purified water until it is colorless, and then elute the product section with 15L of 60% ethanol ...

Embodiment 2

[0065] The method for simultaneously isolating quercetin-3-0-gentiobioside and kaempferol-3-0-gentiobioside from leaves of Asparagus japonicus comprises the following process steps:

[0066] (1) extract

[0067] Weigh 10Kg of Asparagus leaf medicinal material, crush it into a coarse powder of 1-4mm, add 60L of 70% ethanol solution, extract 3 times, 3 hours each time, filter, and combine the filtrates, a total of 90L, is the extract Ⅰ;

[0068] (2) concentrated

[0069] After recovering the ethanol from the extract solution I obtained in step (1), concentrate it to a small volume, and the resulting concentrated solution is 15 L, which is the extract solution II;

[0070] (3) Macroporous resin enrichment and analysis

[0071] Adsorb the extract II obtained in step (2) with a macroporous resin, wash the resin with 80L of purified water until it is colorless, and then elute the product segment with 120L of 65% ethanol solution until the effluent is colorless, collect and combine...

Embodiment 3

[0087] The method for simultaneously isolating quercetin-3-0-gentiobioside and kaempferol-3-0-gentiobioside from leaves of Asparagus japonicus comprises the following process steps:

[0088] (1) extract

[0089] Weigh 20Kg of Asparagus leaf medicinal material, crush it into a coarse powder of 1-4mm, then add 120L of 70% ethanol solution, extract 4 times, 3 hours each time, filter, and combine the filtrates, a total of 185L, is the extract Ⅰ;

[0090] (2) concentrated

[0091] After recovering the ethanol from the extract I obtained in step (1), concentrate it to a small volume, and the obtained concentrate is 30L, which is the extract II;

[0092] (3) Macroporous resin enrichment and analysis

[0093] Adsorb the extract II obtained in step (2) with a macroporous resin, wash the resin with 150L of purified water until it is colorless, and then elute the product segment with 200L of 70% ethanol solution until the effluent is colorless, collect and combine For the eluent, concen...

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Abstract

The invention relates to a method for simultaneously separating quercetin-3-O-gentian diglucoside and kaempferol-3-O-gentian diglucoside from folium sauropi and belongs to the technical field of separation and purification of a compound monomer. According to the invention, the folium sauropi medicinal material is taken as a raw material, and the quercetin-3-O-gentian diglucoside and kaempferol-3-O-gentian diglucoside monomers are acquired according to the steps of ethanol extraction, concentration, macro-porous resin enrichment and elution, polyamide resin enrichment and elution, efficient preparation, liquid chromatography separation and product recycling. According to the invention, through mature processing steps and parameter conditions, the purities of the quercetin-3-O-gentian diglucoside and the kaempferol-3-O-gentian diglucoside in the product respectively reach above 99%; and meanwhile, the method is simple and convenient in operation and is high in separating efficiency, the raw materials are recyclable, and the quercetin-3-O-gentian diglucoside and kaempferol-3-O-gentian diglucoside monomers can be simultaneously separated and prepared in large batch and high purity.

Description

technical field [0001] The invention relates to a method for separating and purifying plant compounds, in particular to a method for simultaneously isolating quercetin-3-O-gentiobioside and kaempferol-3-O-gentiobioside from leaves of asparagus, belonging to traditional Chinese medicine technology field. Background technique [0002] Asparagus leaves Sauropus rostratus It is a plant of the genus Gecko in the family Euphorbiaceae, also known as dragon tongue leaf, dragon taste leaf, dragon leaf, and cow ear leaf. It is mainly distributed in Guangdong, Guangxi, Fujian and other places. It is native to northern Vietnam and cultivated in the Malay Peninsula. It has the effects of clearing away heat and moistening the lungs, reducing phlegm and relieving cough. It is used in the folk to treat diseases such as lung heat, cough, asthma, excessive phlegm, dry mouth, and constipation. The current reports on Longliye are limited to some preliminary discussions, and there are few stud...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H17/07C07H1/08
CPCY02P20/582
Inventor 陈冲李波徐仕银夏柯刘丁
Owner CHENGDU PUSH BIOLOGICAL TECH
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