Novel polypeptide and Periplaneta americana total polypeptide extract capable of promoting tissue repair, and application thereof

Abstract

The invention specifically relates to novel polypeptide and Periplaneta americana total polypeptide extract capable of promoting tissue repair, and application thereof, belonging to the fields of traditional Chinese medicines and crude drugs. The novel polypeptide capable of promoting tissue repair is named as Periplapeptide A and has a structure as shown in a formula I H-Ala-Ala-Pro-Tyr-Ala-His-Val-Ala-Ala-Pro-Tyr-Ala-His-Phe-OH. The Periplaneta americana total polypeptide extract capable of promoting tissue repair is prepared by subjecting the bodies of Periplaneta americana to alcohol extraction, degreasing and enrichment via a reversed-phase material, wherein the mass percentage content of total polypeptide is greater than 50%, and the extract contains Periplapeptide A. The novel polypeptide and the Periplaneta americana total polypeptide extract containing novel polypeptide in the invention can promote propagation of mouse embryo fibroblasts and migration of immortalized human epidermal cells at low concentrations, have low toxic and side effect and good medicinal prospects, and are applicable to preparation of medicines used for treating wounds, burns and ulcers or cosmetics and daily chemical articles used for beautifying the skin.

Description

technical field [0001] The invention relates to the field of traditional Chinese medicine and natural medicine, in particular to a new polypeptide for promoting tissue repair, an extract of total polypeptide of Periplaneta americana and its application. Background technique [0002] The healing process after tissue trauma is a process of proliferation, differentiation, migration and apoptosis of various repair cells. Acute and chronic injuries such as inflammation, ulcers, burns, trauma, surgery, and congenital deformities can cause large areas of skin defects, refractory ulcers, and scars, and trigger many local and systemic reactions, which are very harmful. Peptide drugs have unique advantages in promoting wound healing, preventing scars and treating chronic ulcers. Among them, peptides such as epidermal growth factor (hEGF) and basic fibroblast growth factor (bFGF) have obvious effects of repairing wounds, skin care, anti-wrinkle and anti-aging, but their high preparati...

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Problems solved by technology

[0004] It has been reported in the literature that the ethanol extract of Periplaneta americana can promote mucosal repair (Zhejiang Journal of Integrated Traditional Chinese and Western Medicine, 2012, 9: 689-691) and promote cell migration (Chinese Medicine and Clinic, 2013, 1: 34-36), but has not Clarify its active components or active ingredients; Chinese invention patent 200710067707.4 introduces the method of preparing anti-tumor active parts of Periplaneta americana with reversed-phase materials, but does not clarify the type of chemical components and does not involve the role of promoting tissue repair; Chinese invention patent ZL201210137422. 4 discloses a preparation method of a cockroach polypeptide substance and its anti-herpes virus medical use. Chinese invention patent ZL201210137410.1 introduces a preparation of a cockroach polypeptide and its anti-Gram-positive and Gram-negative bacteria. However, the above-mentioned patent Not only did not clarify the specific chemical composition of the polypeptide part, but also did not involve the application of the total polypeptide and polypeptide monomers in promoting tissue repair

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