Gonococcus, treponema pallidum, chlamydia trachomatis and ureaplasma urealyticum hybrid membrane strip and detection kit

Abstract

The invention discloses a gonococcus, treponema pallidum, chlamydia trachomatis and ureaplasma urealyticum hybrid membrane strip and a detection kit. The kit comprises the hybrid membrane strip; a nitrocellulose membrane of the hybrid membrane strip is immobilized with at least one of the following probes: oligonucleotide sequences for gonococcus, treponema pallidum, chlamydia trachomatis and ureaplasma urealyticum and completely complementary with SEQ ID NO.13, SEQ ID NO.14, SEQ ID NO.15 and SEQ ID NO.16; and amplification primers. By selecting special detection fragments, the kit can detect single microorganism, also can simultaneously detect the presence of 4 kinds of pathogenic microorganisms in a same-tube PCR reaction, and provides simple, fast and cheap selection for screening.

Description

technical field [0001] The invention belongs to the field of medical detection, and in particular relates to a hybrid membrane strip and a detection kit for Neisseria gonorrhoeae, Treponema pallidum, Chlamydia trachomatis and Ureaplasma urealyticum. Background technique [0002] Neisseria gonorrhoeae (NG) / Treponema pallidum (TP) / Chlamydia trachomatis (CT) / Ureaplasma urealyticum (UU) are common sexually transmitted microorganisms in clinic. Neisseria gonorrhoeae mainly cause purulent infections of the genitourinary system, such as gonococcal urethritis and cervicitis. The infection is clinically asymptomatic at the initial stage. If it is not treated in time, the infection can spread locally through the urethra or cervix, causing epididymitis, pelvic inflammatory disease, etc. disease. Ureaplasma urealyticum infection can cause urethritis, cervicitis and bartholinitis; when ascending infection, it can cause prostatitis or epididymitis in men, endometritis, pelvic inflammator...

AI Technical Summary

Problems solved by technology

At present, the common experimental methods used to detect these four pathogenic microorganisms (NG / TP / CT / UU) include culture method, microscope inspection method, enzyme immunoassay method, gold standard method and nucleic acid amplification fluorescent probe method, etc., culture method Although the detection method is accurate and reliable, it is time-consuming, high in cost, low in detection rate, and requires high-standard laboratory conditions and transportation conditions. specific antibody

The method is simple and fast, but the sensitivity and specificity still need to be improved. The nucleic acid amplification fluorescent probe method can judge whether there are corresponding microorganisms in the sample by detecting the nucleic acid sequence specific to the above microorganisms. This method has both sensitivity and specificity, but There are still disadvantages such as high cost, special equipment and professional technical personnel are required to operate, and it is impossible to detect four kinds of pathogenic microorganisms at one time.

When the above method is used for general screening, there may be disadvantages of long period and complicated operation, or the disadvantages of high cost, high requirements for experimental conditions and professional skills

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