Study method using capillary electrophoresis and UPLC-MS (ultra performance liquid chromatography - mass spectrometry) to analyze regulating function of PQQ (pyrroloquinoline quinone) on catecholamine neurotransmitter
A capillary electrophoresis, neurotransmitter technology, applied in the field of biological analysis
Inactive Publication Date: 2017-05-24
JIANGSU INST OF NUCLEAR MEDICINE
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Problems solved by technology
It has been reported in the literature that capillary electrophoresis-indirect electrochemiluminescence can detect the content of catecholamines in urine, but the electrochemiluminescence solution must be renewed every 2 hours of the experiment.
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Embodiment 1
[0051] (1) Capillary selection and pretreatment:
[0052] Choose an uncoated 42cm×50µm quartz capillary column; first rinse the new capillary with methanol for 10 minutes, deionized water for 5 minutes, 1mol / L NaOH solution for 30 minutes, deionized water for 5 minutes, and finally wash with running buffer for 15 minutes; Before each injection, rinse with deionized water and running buffer for 2 minutes;
[0053] Both the running buffer and the sample solution were filtered with a 0.22µm microporous needle filter; the buffer was prepared on the same day;
[0054] The running buffer is: 40mmol / L sodium tetraborate, pH=9.0.
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The invention relates to a study method using capillary electrophoresis and UPLC-MS (ultra performance liquid chromatography - mass spectrometry) to analyze a regulating function of PQQ (pyrroloquinoline quinone) on catecholamine neurotransmitter, and belongs to the technical field of biological analysis. The method comprises the following steps of extracting brain tissues by anion solid-phase extraction, measuring the change of content of catecholamine in a mouse brain of a chronic stress induced depression model and the change of a reaction product of catecholamine, well separating PQQ, NE (noradrenaline), E (epinephrine) and products, and using UPLC-MS to identify the PQQ and the catecholamine reaction product. After proofing by results, the PQQ reacts with NE to generate the PQQNE, but does not react with E. The method has the advantages that the contents of NE in frontal lobe and hippocampus of the chronic stress induced depression model are obviously increased; by applying a certain amount of PQQ group, the content of NE is obviously decreased, and the contents of E in different groups are not obviously different; the PQQ is mainly used for regulating the catecholamine neurotransmitter through regulating the NE, but has no regulating function on E; the guidance meaning on the study of the depression disease mechanism and depression targeted medicine is realized.
Description
technical field [0001] The invention relates to a research method for the regulating effect of pyrroloquinoline quinone (PQQ) on catecholamine neurotransmitters, which belongs to the technical field of bioanalysis. Background technique [0002] Norepinephrine (NE) and epinephrine (E) are important catecholamine neurotransmitters in the human body (see structural formula below). Its structure contains multiple hydroxyl active groups. [0003] [0004] Catecholamine neurotransmitters are secreted by adrenal medulla chromaffin cells and metachromaffin cells, widely distributed in the central nervous system, and are extremely important information transmitters in the central nervous system. The normal content of catecholamines plays an extremely important role in regulating the balance of various physiological functions such as the nervous system and cardiovascular system of the human body, and directly affects the metabolic level of the human body. Certain metabolic disord...
Claims
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IPC IPC(8): G01N27/447G01N30/02
CPCG01N27/447G01N30/02
Inventor 周杏琴顾晓波徐希杰毛师师钦晓峰
Owner JIANGSU INST OF NUCLEAR MEDICINE
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