Decellularizing method for cornea

A decellularization and cornea technology, applied in the field of corneal decellularization, can solve the problems of easily damaged cornea, low efficiency of corneal decellularization, and difficulty in ensuring full contact between cornea and decellularization reagents, so as to improve decellularization efficiency, avoid collisions, The effect of preventing damage

Inactive Publication Date: 2017-05-31
SHENZHEN AINEAR CORNEA ENG
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  • Abstract
  • Description
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Problems solved by technology

[0003] In the prior art, the cornea is usually placed in a container and decellularized by a decellularization reagent under oscillation. During the process, the cornea collides with the wall of the container, which is easy to damage the cornea. , it is difficult to ensure that the cornea is always in full contact with the decellularization reagent, so the decellularization efficiency of the cornea is low

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  • Decellularizing method for cornea
  • Decellularizing method for cornea

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Embodiment Construction

[0023] A corneal decellularization method provided by an embodiment of the present invention will be described in detail below with reference to the accompanying drawings.

[0024] The embodiment of the present invention provides a method for corneal decellularization, see figure 1 ,include:

[0025] Step 1) Put the cornea and the decellularization reagent into a vacuum bag, remove the air, and obtain the vacuum-packed cornea;

[0026] Step 2) The vacuum-packed cornea is placed on a shaker for shaking to decellularize the cornea.

[0027] The embodiment of the present invention provides a method for decellularization of the cornea. By putting the cornea and the decellularization reagent into a vacuum bag and removing the air, a vacuum-packed cornea is obtained. When the cornea is decellularized, the vacuum-packed cornea Placed on a shaker for shaking, this method is simple and easy to operate, the cornea can be fully contacted with the decellularization reagent, and at the s...

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Abstract

The invention relates to the technical field of cornea decellularization, in particular to a decellularizing method for cornea. The decellularizing method for the cornea is simple and easy to operate and can enhance the cornea decellularizing efficiency under the mildest environment. Defects that the cornea is easily damaged because the cornea has a collision with a solid container during decellularizing the cornea under an oscillation condition and the decellularizing efficiency is lower in the prior art are overcome. The embodiment of the invention provides the decellularizing method for the cornea. The decellularizing method for the cornea comprises the steps: step 1) putting the cornea and a decellularizing reagent into a vacuum bag and exhausting the air in the vacuum bag to obtain a vacuum-packaged cornea; step 2) placing the vacuum-packaged cornea in a shaker to be vibrated and decellularizing the cornea. The decellularizing method provided by the embodiment of the invention is applied to the cornea decellularization.

Description

technical field [0001] The invention relates to the technical field of corneal decellularization, in particular to a corneal decellularization method. Background technique [0002] In recent years, with the rapid development of decellularization technology, the corneal stroma obtained after decellularization of xenogeneic corneas to remove immunogenic substances has similar tissue structure, biophysical properties and optical properties to human corneal stroma, and has become the first choice for human corneal transplantation. The best substitutes, especially the corneal stroma obtained by decellularization of animal cornea, have a wide range of sources, which provides a guarantee for the clinical application of corneal transplantation. [0003] In the prior art, the cornea is usually placed in a container and decellularized by a decellularization reagent under oscillation. During the process, the cornea collides with the wall of the container, which is easy to damage the co...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36
CPCA61L27/3604A61L27/3691A61L2430/16A61L2430/40
Inventor 张诗雯张爱兵左楠
Owner SHENZHEN AINEAR CORNEA ENG
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