Preparation method of composite IgY antibody against common cold virus, streptococcus pneumonia and staphylococcus and application thereof

A staphylococcus and cold virus technology, applied in antiviral immunoglobulins, antiviral agents, antibacterial immunoglobulins, etc., can solve the problems of ineffectiveness, non-recognition of antigens, limited antigenic determinants, etc., and achieve good curative effect, Less dosage, quick effect

Active Publication Date: 2017-05-31
黑龙江省安碧捷生物科技有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Virus vaccines are recombinant subunit vaccines or attenuated virus vaccines for human infection. Due to long-term infection of the human body, people often develop tolerance to their antigens and do not recognize them, or the recognized antigenic determinants are limited, so the immune effect is not good, and the preventive effect not ideal, almost ineffective

Method used

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  • Preparation method of composite IgY antibody against common cold virus, streptococcus pneumonia and staphylococcus and application thereof
  • Preparation method of composite IgY antibody against common cold virus, streptococcus pneumonia and staphylococcus and application thereof
  • Preparation method of composite IgY antibody against common cold virus, streptococcus pneumonia and staphylococcus and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0032] Preparation of 2000 grams of anti-cold virus and Streptococcus pneumoniae, staphylococcus composite IgY antibody water extract production process:

[0033] (1) Preparation of immunogen:

[0034] Influenza A Type 1 Virus 10, Influenza A Type 3 Virus 20, Influenza B Virus 5, Rhinovirus 10, Coronavirus 10, Respiratory Syncytial Virus 5, Adenovirus 5, Pneumonia Chain 15 parts of cocci and 20 parts of Staphylococcus aureus were mixed and inactivated with carbolic acid;

[0035] Mix 500ml of virus liquid + bacteria liquid with 500ml of Freund's complete adjuvant, stir and emulsify with a high-speed mixer, and make the immunogen of Freund's complete adjuvant in water-in-oil, and the preparation of the immunogen of Freund's incomplete adjuvant is : Mix 500ml of virus liquid + bacteria liquid with 500ml of Freund's incomplete adjuvant, stir and emulsify with a high-speed mixer, and make water-in-oil Freund's incomplete adjuvant immunogen;

[0036] (2) Immune laying hens: Use a...

Embodiment 2

[0041] Anti-cold virus, Streptococcus pneumoniae, Staphylococcus compound IgY antibody nasal drops: Anti-cold virus, Streptococcus pneumoniae, Staphylococcus compound IgY antibody water extract 0.20%, peach essence 0.60%, aspartame 0.12%, distilled water Replenish to 100ml;

[0042] Add 80ml of distilled water into the beaker, then add the above ingredients in sequence, and finally add distilled water to 100ml, filter and sterilize through a 0.22μ sterile microporous membrane, and aseptically fill it into a bottle of sterilized nasal drops.

Embodiment 3

[0044] Anti-cold virus and Streptococcus pneumoniae, Staphylococcus compound IgY antibody oral spray, aerosol:

[0045] Anti-cold virus and Streptococcus pneumoniae, Staphylococcus compound IgY antibody water extract 0.20%, peppermint oil 0.06%, orange essence 0.60%, peach essence 0.40%, aspartame 0.12%, distilled water supplemented to 100ml;

[0046] Add 60ml of distilled water into the beaker, then add the above ingredients in sequence, and finally add the water extract of anti-cold virus, Streptococcus pneumoniae, and Staphylococcus complex IgY antibody, then add distilled water to 100ml, filter through a 0.22μ sterile microporous filter membrane sterile, aseptically filled into sterile spray and aerosol cans.

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Abstract

The invention discloses a preparation method of a composite IgY antibody against common cold virus, streptococcus pneumonia and staphylococcus. The preparation method comprises the following steps: mixing the common cold virus, streptococcus pneumonia and staphylococcus as antigens to obtain Freund's complete adjuvant immunogen and Freund's incomplete adjuvant immunogen; performing subcutaneous and intramuscular immunization of a laying hen; extracting the IgY antibody from the egg yolk of the immunized hen by a water dilution method; and purifying the IgY antibody by a two-step ammonium sulfate salt fractionation method to obtain a composite IgY antibody against the common cold virus, streptococcus pneumonia and staphylococcus. A product prepared from the composite IgY antibody with specific resistance against the common cold virus, streptococcus pneumonia and staphylococcus can be used for controlling the upper respiratory infection inflammation such as common cold, rhinitis, sinusitis, tonsillitis, sphagitis and bronchitis caused by the common cold virus, streptococcus pneumonia and staphylococcus and the viruses and bacteria with the same antigens.

Description

technical field [0001] The invention relates to a preparation method and application of compound IgY antibody against cold virus, streptococcus pneumoniae and staphylococcus. Background technique [0002] The onset of upper respiratory tract viral infection and secondary bacterial infection has no time or seasonal characteristics, and occurs all year round, and the incidence group is not selective, everyone can develop the disease, and it can occur repeatedly. A cold is an infection of the upper respiratory tract. Colds can usually be caused by seasonal influenza viruses and non-seasonal rhinoviruses, coronaviruses, respiratory syncytial virus, and adenoviruses. Secondary infection of the upper respiratory tract or external Streptococcus pneumoniae and Staphylococcus lead to inflammation of the upper respiratory tract. There is currently no effective drug available for viral infection, and premature use of antibiotic prophylaxis is not allowed, and may even lead to the emer...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/12C07K16/10C07K16/08C07K16/02A61K39/42A61K39/40A61P31/04A61P31/16A61P31/14A61P31/20
CPCA61K2039/507A61K2039/541A61K2039/542A61K2039/543C07K16/02C07K16/081C07K16/10C07K16/1009C07K16/1018C07K16/1027C07K16/1271C07K16/1275
Inventor 胡国柱姚善龙胡微煦周文芸张进聂荣庆
Owner 黑龙江省安碧捷生物科技有限责任公司
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