Unlock instant, AI-driven research and patent intelligence for your innovation.

Method for cultivating Trichoderma using blue-green algae mud

A technology of blue-green algae mud and Trichoderma, applied in the fields of resource recycling and bioengineering, can solve the problem of high cultivation costs, achieve the effect of improving disease resistance and promoting sustainable development

Active Publication Date: 2021-02-09
JIANGNAN UNIV
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, the cultivation of biocontrol Trichoderma mainly relies on grain raw materials, such as corn, potato, etc., and its cultivation cost is relatively high.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The preparation of embodiment 1 blue-green algae mud hydrolyzate

[0028] Take 50g of dry-based cyanobacteria mud, stir and redissolve it with water at a mass ratio of 6:1, break the wall at a high speed for 10 minutes in a homogenizer, and add 1% neutral protease (based on dry-based cyanobacterial mud) to the liquid. Benchmark), hydrolyzed at 45°C for 2 hours; then added 3% alkaline protease (based on the dry basis of blue-green algae mud), and hydrolyzed at 55°C for 3 hours to obtain a hydrolyzed solution of blue-green algae mud, and the degree of proteolysis was measured to be 65%.

Embodiment 2

[0029] The preparation of embodiment 2 blue-green algae mud hydrolyzate

[0030] Take 50g of dry-based cyanobacteria mud, stir and redissolve it with water at a mass ratio of 5:1, break the wall at a high speed for 10 minutes in a homogenizer, and add 3% neutral protease (based on dry-based cyanobacteria mud) to the liquid. standard), hydrolyze at 40°C for 4 hours; then add 5% alkaline protease (based on the dry basis of blue-green algae mud), and hydrolyze at 55°C for 2 hours to obtain a hydrolyzed solution of blue-green algae mud.

Embodiment 3

[0031] The preparation of embodiment 3 blue-green algae mud hydrolyzate

[0032] Take 50g of dry-based cyanobacteria mud, stir and redissolve it with water at a mass ratio of 10:1, break the wall at a high speed for 10 minutes in a homogenizer, and add 3% neutral protease (based on dry-based cyanobacterial mud) to the liquid. standard), hydrolyze at 50°C for 4 hours; then add 2% alkaline protease (based on the dry basis of blue-green algae mud), and hydrolyze at 50°C for 5 hours to obtain a hydrolyzed solution of blue-green algae mud.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a method for cultivating Trichoderma by using blue-green algae mud, comprising the following steps: redissolving the blue-green algae mud and performing wall-breaking treatment, then adding neutral protease, hydrolyzing at 40-50°C for 2-4 hours, and then adding alkali Protease, hydrolyzing at 50-55°C for 2-5 hours to obtain hydrolyzed cyanobacterial mud; preparing the hydrolyzed cyanobacterial mud into a fermentation medium, inoculating Trichoderma into the fermentation medium for fermentation and cultivation. The invention uses the blue-green algae mud as a raw material, utilizes the blue-green algae mud as a resource, obtains the blue-green algae hydrolyzate rich in short peptides / amino acids after protease treatment, and prepares the cultivated trichoderma into a culture medium, which can be directly used in agricultural production.

Description

technical field [0001] The invention relates to the technical fields of resource recycling and bioengineering, in particular to a method for cultivating Trichoderma by using blue-green algae mud. Background technique [0002] Cyanobacteria are Gram-negative, non-flagellated, chlorophyll-a-containing, non-chloroplast-forming prokaryotes capable of aerobic photosynthesis. In recent years, there have been frequent outbreaks of blue-green algae in Taihu Lake, Dianchi Lake, Chaohu Lake and other places, and a large number of blue-green algae have been salvaged, consuming a lot of manpower and financial resources. However, the water content of the blue-green algae picked up reaches more than 98%, which cannot be preserved. If it is not processed in time, it will stink, and then cause secondary pollution. [0003] At present, common treatment methods for cyanobacteria mud include: (1) composting treatment: it can degrade algae toxins, but farmers don’t like to use them, and the us...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12R1/885
CPCC12N1/14
Inventor 詹晓北吴剑荣刘和郑志永
Owner JIANGNAN UNIV