Graphene oxide particle for targeted drug delivery and preparation method of graphene oxide particle

A technology for targeted drug delivery and graphene, applied in the field of graphene oxide particles for targeted drug delivery and the preparation thereof, can solve the problems of high drug loading, long preparation period, and complex preparation of graphene oxide particles, and achieves a material Low cost, by-products and the effects of simple handling and operation

Inactive Publication Date: 2017-06-09
SHANGHAI APPLIED TECHNOLOGIES COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The graphene oxide particles used for targeted drug delivery of the present invention have a high drug loading capacity; at the same time, the preparation method solves the technical problems such as complicated preparation and long preparation period in the traditional preparation process, and avoids a large loss of products

Method used

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  • Graphene oxide particle for targeted drug delivery and preparation method of graphene oxide particle
  • Graphene oxide particle for targeted drug delivery and preparation method of graphene oxide particle
  • Graphene oxide particle for targeted drug delivery and preparation method of graphene oxide particle

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Effect test

Embodiment 1

[0026] 1. Weigh 100mg of PEI (polyethyleneimine), dissolve it in 5ml of deionized water and mix it ultrasonically to obtain a 2% PEI aqueous solution;

[0027] 2. Weigh 0.5000g of graphite powder and 0.5000g of sodium nitrate into a 250ml three-neck flask, add 46ml of concentrated sulfuric acid under ice bath conditions, and mechanically stir at 800r / min for 0.5 hours;

[0028] 3. Weigh 3.000g of potassium permanganate, slowly add it into the three-neck flask under ice bath condition, add time over one hour, mechanically stir at 800r / min under ice bath condition for 2 hours; Stir for 1 hour, add 23ml of deionized water, control the temperature at 95-98°C, stir mechanically for 0.5 hours, add a large amount of 70ml of deionized water, then add 30% hydrogen peroxide dropwise until the solution turns golden yellow, and place the reaction solution in a large beaker overnight, pour off the supernatant, adjust the pH to weak acidity by precipitation, and obtain graphene oxide GO; af...

Embodiment 2

[0034] 1. Weigh 100mg of PEI (polyethyleneimine), dissolve it in 5ml of deionized water and mix it ultrasonically to obtain a 2% PEI aqueous solution;

[0035] 2. Weigh 0.2500g of graphite powder and 0.2500g of sodium nitrate into a 250ml three-neck flask, add 23ml of concentrated sulfuric acid under ice bath conditions, and mechanically stir at 800r / min for 0.5 hours;

[0036] 3. Weigh 1.5000g of potassium permanganate, and slowly add it into the three-neck flask under ice bath conditions. Stir for 1 hour, add 12ml of deionized water, control the temperature at 95-98°C, stir mechanically for 0.5 hours, add a large amount of deionized water 35ml, then add 30% hydrogen peroxide dropwise until the solution turns golden yellow, and place the reaction solution in a large beaker overnight, pour off the supernatant, adjust the pH to weak acidity by precipitation, and obtain graphene oxide GO; after ultrasonic dispersion of graphene oxide GO for 2 hours, add 200 mg of EDC, magnetical...

Embodiment 3

[0042] 1. Weigh 200mg of PEI (polyethyleneimine), dissolve it in 5ml of deionized water and mix it ultrasonically to obtain 4% PEI aqueous solution;

[0043] 2. Weigh 0.5000g of graphite powder and 0.5000g of sodium nitrate into a 250ml three-neck flask, add 46ml of concentrated sulfuric acid under ice bath conditions, and mechanically stir at 800r / min for 0.5 hours;

[0044]3. Weigh 3.000g of potassium permanganate, slowly add it into the three-neck flask under ice bath condition, add time over one hour, mechanically stir at 800r / min under ice bath condition for 2 hours; Stir for 1 hour, add 23ml of deionized water, control the temperature at 95-98°C, stir mechanically for 0.5 hours, add a large amount of 70ml of deionized water, then add 30% hydrogen peroxide dropwise until the solution turns golden yellow, and place the reaction solution in a large beaker overnight, pour off the supernatant, adjust the pH to weak acidity by precipitation, and obtain graphene oxide GO; after...

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Abstract

The invention discloses a graphene oxide particle for targeted drug delivery and a preparation method thereof. The specific steps of the method of the present invention are as follows: firstly, a polyethyleneimine solution of a certain concentration is prepared, graphite powder is weighed, and polyethyleneimine-modified graphene oxide particles are prepared in one step by an improved Hummer method. Then disperse the prepared above-mentioned particles in deionized water, add dropwise a certain concentration of activated hyaluronic acid under stirring, keep away from light and stir for a certain period of time, put them in a dialysis bag, freeze and dry after dialysis to obtain a target Graphene oxide particles for drug delivery. The yield of the present invention is relatively high, and the particle size range is about 200-400nm. Through the experimental study on the loading of the derivative (CA4P) of the second-generation tumor blood vessel targeting destroyer combustin (CA4), the highest drug loading rate can reach 34%. Under the electron microscope, the morphology is stable and layered.

Description

technical field [0001] The invention relates to the technical field of medical carriers, in particular to a graphene oxide particle for targeted drug delivery and a preparation method thereof. Background technique [0002] Graphene oxide is a common non-toxic and biocompatible material. In recent years, graphene oxide has been used in the research of drug delivery. [0003] Since graphene oxide can be used for drug carrier delivery, people began to modify graphene oxide to achieve the purpose of targeted drug delivery. The traditional modification method is to prepare graphene oxide first, and then through a series of physical or chemical modifications to make it have groups that can be grafted with targeting ligands, so as to achieve the purpose of targeted drug delivery. However, this preparation method will cause a large loss of the product graphene oxide in the processing stage of the preparation. At the same time, the particle size of the prepared particles is too larg...

Claims

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Application Information

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IPC IPC(8): C01B32/198A61K47/32A61K47/36A61K47/04A61K31/09A61P35/00
CPCA61K9/5161A61K9/5115A61K9/5138A61K31/09C01P2002/88C01P2004/03C01P2004/62
Inventor刘晴吴泓杉黄凯郭文静刘维俊
OwnerSHANGHAI APPLIED TECHNOLOGIES COLLEGE