Pachymaran extraction method

An extraction method and Poria polysaccharide technology, applied in the field of biological separation, can solve problems such as low extraction efficiency, and achieve the effects of facilitating digestion and absorption, small molecular weight, and avoiding residue and pollution

Inactive Publication Date: 2017-06-09
TIANJIN ZHENBANG AQUACULTURE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention aims at the technical defects of the prior art, and provides a method for extracting polysaccharides from poria cocos, so as to solve the technical problem of low extraction efficiency existing in the prior art

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] A method for extracting polysaccharides from Poria cocos, comprising the following steps:

[0018] 1) Pulverizing the Poria cocos raw material to obtain Poria cocos dry powder, and then freeze-drying to obtain Poria cocos defatted dry powder;

[0019] 2) Add water to Poria cocos defatted dry powder to prepare a 20 g / L suspension and shake it for 1 hour, then add sodium citrate of 2% of the total weight of the Poria cocos defatted dry powder to the suspension, adjust the pH of the suspension to 8.5, Extract at 40°C for 4 hours, and then perform a boiling water bath on the extract for 10 minutes;

[0020] 3) Then keep the suspension at 50°C for a certain period of time, add 2% alkaline protease by weight of poria cocos defatted dry powder, adjust the pH to 8.5, adjust the pH to 8.5 every 20 minutes, extract for 6 hours, and put it in a boiling water bath to extinguish After enzymatic activity for 10 minutes, the above-mentioned extract was centrifuged at 5000 r / min for 2...

Embodiment 2

[0022] A method for extracting polysaccharides from Poria cocos, comprising the following steps:

[0023] 1) Pulverizing the Poria cocos raw material to obtain Poria cocos dry powder, and then freeze-drying to obtain Poria cocos defatted dry powder;

[0024] 2) Add water to Poria cocos defatted dry powder to prepare a suspension of 30 g / L and shake it for 1 hour, then add sodium citrate of 4% of the total weight of the Poria cocos defatted dry powder to the suspension, adjust the pH of the suspension to 9, Extract at 50°C for 6 hours, and then perform a boiling water bath on the extract for 15 minutes;

[0025] 3) Then keep the suspension at 60°C for a certain period of time, add 4% alkaline protease by weight of poria cocos defatted dry powder, adjust the pH to 9.5, adjust the pH to 9.5 every 30 minutes, extract for 7 hours, put it in a boiling water bath to extinguish After enzymatic activity for 10 minutes, the above-mentioned extract was centrifuged at 5000 r / min for 20 m...

Embodiment 3

[0029] A method for extracting polysaccharides from Poria cocos, comprising the following steps:

[0030] 1) Pulverizing the Poria cocos raw material to obtain Poria cocos dry powder, and then freeze-drying to obtain Poria cocos defatted dry powder;

[0031] 2) Add water to Poria cocos defatted dry powder to prepare a 25 g / L suspension and shake it for 1 hour, then add 3% sodium citrate of the total weight of the Poria cocos defatted dry powder to the suspension, adjust the pH of the suspension to 8.8, Extract at 45°C for 5 hours, and then perform a boiling water bath on the extract for 12 minutes;

[0032] 3) Then keep the suspension at 55°C for a certain period of time, add 3% alkaline protease by weight of poria cocos degreased dry powder, adjust the pH to 9, adjust the pH to 9 every 25 minutes, extract for 6.5 hours, and place in a boiling water bath Inactivate the enzyme for 10 minutes, centrifuge the above extract at 5000 r / min for 20 minutes, and take the supernatant t...

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PUM

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Abstract

The invention provides a pachymaran extraction method. According to the method, tuckahoe dry powder is subjected to freeze-drying treatment, and hydrolyzed sequentially by sodium citrate and alkaline proteinase. The method avoids residues and pollution in the chemical polysaccharide preparation process. The pachymaran prepared by the method has the advantages of high hydrolysis degree and lower molecular weight of the polysaccharide segment. The method has the advantages of ingenious technical concept and high product purity, and thus, has outstanding technical advantages.

Description

technical field [0001] The invention relates to the technical field of bioseparation, in particular to a method for extracting Poria cocos polysaccharide. Background technique [0002] Poria cocos, the name of traditional Chinese medicine. It is the dry sclerotia of Polyporaceae fungus Poria cocos (Schw.) Wolf. More than 7 to 9 months of excavation, remove the sand after digging, pile up to "sweat", spread out to dry the surface, and then "sweat", repeat several times until wrinkles appear and most of the internal water is lost, then dry in the shade , called "Poria"; or cut fresh Poria according to different parts, dried in the shade, called "Poria Block" and "Poria Tablet" respectively. [0003] In the prior art, the extraction of Poria cocos polysaccharide still has the technical defects of low purity and complicated process. Contents of the invention [0004] The present invention aims at the technical defects of the prior art, and provides a method for extracting p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
CPCC08B37/00C08B37/0003Y02P20/54
Inventor 季延滨李涛侯继明
Owner TIANJIN ZHENBANG AQUACULTURE
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