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Determination of vitamin K by an isotope dilution ultra-high performance liquid chromatography-mass spectrometry 1 Methods

A technique of chromatographic mass spectrometry and ultra-high performance liquid phase, which is applied in the field of isotope dilution ultra-high performance liquid chromatography-mass spectrometry for the determination of vitamin K1, and can solve the problems that the diagnosis cannot be fully reflected.

Active Publication Date: 2020-07-07
HANGZHOU BAICHEN MEDICAL LAB CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Vitamin K 1 The detection method can be applied in many aspects, such as scientific research, simple data detection, etc. It is not limited to diagnosis, and clinical disease diagnosis is a comprehensive evaluation. This indicator cannot fully reflect the diagnosis of a certain disease. It is only used as a auxiliary reference

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  • Determination of vitamin K by an isotope dilution ultra-high performance liquid chromatography-mass spectrometry <sub>1</sub> Methods
  • Determination of vitamin K by an isotope dilution ultra-high performance liquid chromatography-mass spectrometry <sub>1</sub> Methods
  • Determination of vitamin K by an isotope dilution ultra-high performance liquid chromatography-mass spectrometry <sub>1</sub> Methods

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Embodiment Construction

[0051] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0052] Instruments and reagents:

[0053] API 4000 triple quadrupole mass spectrometer (USA, Applied Biosystem Company) is equipped with electrospray ion source; Waters ultra-high performance liquid chromatography (USA, Waters Company) is equipped with binary high-pressure pump, automatic sampler, column oven ; H1650R desktop high-speed refrigerated centrifuge (China, Shanghai Luxiangyi Centrifuge Instrument Co., Ltd.); BT125D electronic balance (Germany, Sart...

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Abstract

The invention discloses a method for rapidly and sensitively combined-testing Vitamin K1 by isotope dilution ultra-high performance liquid chromatography, wherein the method includes steps of (1), preparing reference substance solution of Vitamin K1 and isotope internal standard solution of Vitamin K1; (2), setup up a standard curve of the Vitamin K1; (3), sampling chromatogram of a sample solution to be tested; (4), determining the concentration of the Vitamin K1 in the sample to be tested. When the method for combined-testing Vitamin K1 by isotope dilution ultra-high performance liquid chromatography is used for test the Vitamin K1 in serum, the method is simple and rapid, high in accuracy, strong in specificity, high in accuracy, and good in sensitivity.

Description

technical field [0001] The invention relates to a rapid and sensitive determination of vitamin K by isotope dilution ultra-high performance liquid chromatography-mass spectrometry 1 Methods. Background technique [0002] Vitamin K 1 Also known as phytonadione, it is a fat-soluble vitamin and is an essential substance for the synthesis of prothrombin in the human liver, which can promote the precursor of prothrombin (factor II) and other coagulation factors (factor VIII, IX, X) Substance carboxylation reaction, and then converted into prothrombin and the corresponding coagulation factors. Clinically, it is commonly used in the treatment of skin and mucous membrane bleeding, internal bleeding, bleeding after trauma surgery, neonatal bleeding and other deep tissue bleeding. In addition, vitamin K 1 It is also involved in bone metabolism and cell growth, and has the effects of analgesia and relieving bronchospasm. Vitamin K 1 The detection method can be applied in many asp...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 彭军陈从艳吴超超高强
Owner HANGZHOU BAICHEN MEDICAL LAB CO LTD