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Experimental method for simulating oral environment, material for environment, and application of material

An experimental method and environmental technology, applied in the field of oral medical materials, can solve problems such as elevation, reduction, and minimal tooth protection, and achieve the effect of promoting remineralization, high feasibility, and simple operation

Inactive Publication Date: 2017-06-23
SICHUAN UNIV
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0002] The occurrence of secondary caries is the main reason for the failure of resin filling restoration. The incidence of primary caries and secondary caries in high caries risk groups is much higher than that in normal people; saliva can promote the remineralization of demineralized tooth hard tissue , plays a crucial role in the process of inhibiting the progression of caries; saliva has a strong buffer capacity due to its rich bicarbonate and other components, which can penetrate into bacterial biofilms, neutralize the acidic products of cariogenic bacteria, and increase The pH value of the local microenvironment; and, saliva is rich in calcium and phosphorus ions, which can reduce the solubility of hydroxyapatite, the main component of dental hard tissue, and promote its remineralization; however, about 30% of the population will suffer from Symptoms of dry mouth, the reasons include excessive intake of alcohol, the use of certain drugs, malnutrition, certain systemic diseases, salivary gland dysfunction, etc.; due to the reduction of total salivary secretion, saliva flow may drop to normal levels 15%-33%, which means that the amount of saliva that can enter the acidic products of nuclear bacteria in the biofilm and provide calcium and phosphorus particles is greatly reduced; the reduction of saliva mainly occurs in the elderly. Studies have shown that the incidence of root caries in the United States increases with Increased with age; among them, the incidence of root caries in young people is only 7%; while the incidence rate in elderly people over 75 years old can be as high as 56%; in addition, patients who receive radiation therapy for head and neck tumors will develop The sharp reduction in saliva volume leads to the occurrence of violent caries; at present, there is no clinically effective anti-caries method for patients with reduced saliva volume, and only some saliva substitutes can be used to relieve the symptoms of dry mouth, but for teeth The protective effect of these populations is very small; therefore, it is of high clinical significance to find materials and methods that can protect the dental hard tissues of this group of people; currently there are no very effective experimental methods and materials

Method used

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  • Experimental method for simulating oral environment, material for environment, and application of material
  • Experimental method for simulating oral environment, material for environment, and application of material

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Experimental program
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Effect test

Embodiment 1

[0050] Experiments were carried out using the above-mentioned simulated oral environment, and the pH of the four groups of lactic acid solutions were measured at 15min, 60min, 3h and 24 hours after the liquid was changed on the 1st, 3rd, 5th, 7th, 10th, 14th and 21st day respectively; the test results were as follows Figure 1-4 As shown; the pH value of the lactic acid solution in the PAMAM group and the control group was basically constant at 4; the pH value of the NACP group and the PAMAM+NACP group was basically the same.

[0051] The lactic acid solution of NACP group and PAMAM+NACP group rose to 6.0 at 15 minutes on the first day; the lactic acid solution of NACP group and PAMAM+NACP group rose to 6.5 at 60 minutes on the first day; The lactic acid solution rose to 6.9 at 3 hours on the first day; the pH value of the solution measured from the first day to the 21st day decreased slightly; the pH value of the NACP group and PAMAM+NACP group rose to 15 minutes on the 21st d...

Embodiment 2

[0053] The above-mentioned simulated oral environment was used for the experiment, and the concentration of calcium and phosphorus ions in the four groups of lactic acid solutions were measured by spectrophotometry after the 1st, 3rd, 5th, 7th, 10th, 14th and 21st day respectively; 1ml of the solution was taken for each measurement. ; Measurement results such as Figure 5 and Figure 6 As shown; the concentrations of calcium and phosphorus ions in the PAMAM group and the control group were basically constant at 0; the concentrations of calcium and phosphorus ions in the NACP group and the PAMAM+NACP group were basically the same.

[0054] The calcium ion concentration in the NACP group and the PAMAM+NACP group solution was 37mmol / L on the first day and 6mmol / L on the 21st day, and the phosphorus ion concentration was 15mmol / L on the first day and 3mmol / L on the 21st day; NACP's resin can release the calcium and phosphorus ions necessary for the remineralization of the compres...

Embodiment 3

[0056] The above-mentioned simulated oral environment was used for the experiment. After 21 days of the experiment, each sample was cut into two halves with a diamond blade, one half was used to detect the transverse section (perpendicular to the direction of the dentin tubules), and the other half was used to detect the longitudinal section (parallel to the dentin tubule direction). The direction of the small tube); each sample was soaked in PBS solution containing 1% glutaraldehyde and stored at 4°C for 4 hours, then dehydrated with gradient ethanol, washed with 100% hexamethyldisilamine and sprayed with gold; observed by scanning electron microscope At the same time, energy dispersive spectrometer was used to detect the chemical element components of the sample; the scanning electron microscope detection results were as follows: Figure 7 and Figure 8 Shown; chemical element composition results are as follows Figure 9 and Figure 10 shown; Figure 7 Figure A in the mid...

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Abstract

The invention discloses an experimental method for simulating an oral environment, a material for the environment, and application of the material. The experimental method comprises: preparing a lactic acid solution and treating a demineralization dentin sample. NACP resin comprises 100 parts of resin containing nano amorphous calcium phosphate, 0.2 part of camphorquinone, and 0.8 part of p-N,N-dimethylamino isoamyl benzoate. The cooperation with a PAMAM solution has the effects of buffering the pH value of the lactic acid solution, releasing calcium phosphate particles and promoting remineralization of the demineralization dentin sample. An extreme oral environment where saliva does not exist totally is simulated, thereby facilitating prevention and treatment of dental caries of a patient suffering from decrease of saliva quantity. The experimental method can be clinically applied to prevention and treatment of the dental caries of the patient suffering from the decrease of the saliva quantity, and is simple in operation and high in feasibility.

Description

technical field [0001] The invention relates to the field of stomatology materials, in particular to an experimental method for simulating an oral cavity environment and its application to the environment. Background technique [0002] The occurrence of secondary caries is the main reason for the failure of resin filling restoration. The incidence of primary caries and secondary caries in high caries risk groups is much higher than that in normal people; saliva can promote the remineralization of demineralized tooth hard tissue , plays a crucial role in the process of inhibiting the progression of caries; saliva has a strong buffer capacity due to its rich bicarbonate and other components, which can penetrate into bacterial biofilms, neutralize the acidic products of cariogenic bacteria, and increase The pH value of the local microenvironment; and, saliva is rich in calcium and phosphorus ions, which can reduce the solubility of hydroxyapatite, the main component of dental h...

Claims

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Application Information

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IPC IPC(8): G01N33/00G01N1/28
CPCG01N1/28G01N33/00
Inventor 李继遥梁坤能陶思颖周学东徐华琨
Owner SICHUAN UNIV
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