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Test paper used for detecting fenpropathrin, and applications thereof
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A technology of fenpropathrin and test paper, which is applied in the field of rapid detection, can solve the problems of complicated and time-consuming detection sample processing, and achieve the effects of short detection time, simple storage and strong specificity
Active Publication Date: 2017-07-04
贵州勤邦食品安全科学技术有限公司
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[0003]
At present, the detection of fenpropathrin residues is mainly carried out by liquid chromatography, gas chromatography and gas chromatography-mass spectrometry. The instrument method has the advantages of high detection sensitivity and strong specificity. Requires expensive large-scale instruments and equipment, equipped with professional testing technicians, and its promotion and application are limited
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Embodiment 1
[0024] Embodiment 1 detects the preparation of the test paper of fenpropathrin
[0025] The preparation method of this test paper mainly comprises the following steps:
[0027] b) preparing a reaction membrane 3 with a detection line 5 coated with a fenpropathrin hapten-carrier protein conjugate and a quality control line 6 coated with a goat anti-mouse anti-antibody;
[0028] c) Assemble the conjugate release pad 2 prepared in a) and b), the reaction membrane 3 , the sample absorbent pad 1 , the water absorbent pad 4 and the bottom plate 7 into a test paper.
[0029] The following step-by-step detailed description:
[0030] 1. Preparation of fenpropathrin hapten, see the synthetic route figure 1
[0074] 2, detect with the test paper of embodiment 1
[0075] Use a straw to absorb the tea sample solution to be tested and add 2 to 3 drops vertically to the sample hole. When the liquid flows, start timing, react for 5 minutes, and judge the result. Other times are invalid.
[0076] 3. Analyze the test results, see Figure 5
[0077] Negative (-): Both the T l...
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Abstract
The invention discloses a test paper used for detecting fenpropathrin, and applications thereof. The test paper used for detecting fenpropathrin comprises a sample absorption pad (1), a combined compound release pad (2), a reaction membrane (3), a water absorption pad (4), and a base plate (7); the sample absorption pad (1), the combined compound release pad (2), the reaction membrane (3), and the water absorption pad (4) are glued onto the base plate (7) successively; the reaction membrane (3) is provided with a fenpropathrinhapten-carrier protein conjugate coated detection line (5), and a goat anti-mouse antibody coated reference line (6); the combined compound release pad (2) is coated with a fenpropathrin monoclonalantibody-colloidal gold marker. The test paper can be used for detecting residue fenpropathrin in tea, operation is simple, sensitivity is high, detection time is short, cost is low, and the test paper is suitable for screening and on-site monitoring of a large amount of samples.
Description
technical field [0001] The invention belongs to the technical field of rapid detection, and in particular relates to a test paper for detecting fenpropathrin, which is especially suitable for detecting fenpropathrin in tea samples. Background technique [0002] Fenpropathrin, α-cyano-phenoxybenzyl-2,2,3,3-tetramethylcyclopropane carboxylate, also known as fenpropathrin, is a kind of pyrethroids Insecticide and acaricide. Fenpropathrin is a nerve agent that can act on the nervous system of insects, causing them to be overexcited, paralyzed and dead. In recent years, pyrethroid pesticides have been widely used due to their high efficiency in killing insects. Among them, fenpropathrin has been detected in food, and fenpropathrin is moderately toxic to higher animals. [0003] At present, the detection of fenpropathrin residues is mainly carried out by liquid chromatography, gas chromatography and gas chromatography-mass spectrometry. The instrument method has the advantages o...
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