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Test paper used for detecting fenpropathrin, and applications thereof

A technology of fenpropathrin and test paper, which is applied in the field of rapid detection, can solve the problems of complicated and time-consuming detection sample processing, and achieve the effects of short detection time, simple storage and strong specificity

Active Publication Date: 2017-07-04
贵州勤邦食品安全科学技术有限公司
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the detection of fenpropathrin residues is mainly carried out by liquid chromatography, gas chromatography and gas chromatography-mass spectrometry. The instrument method has the advantages of high detection sensitivity and strong specificity. Requires expensive large-scale instruments and equipment, equipped with professional testing technicians, and its promotion and application are limited

Method used

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  • Test paper used for detecting fenpropathrin, and applications thereof
  • Test paper used for detecting fenpropathrin, and applications thereof
  • Test paper used for detecting fenpropathrin, and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1 detects the preparation of the test paper of fenpropathrin

[0025] The preparation method of this test paper mainly comprises the following steps:

[0026] a) preparing conjugate release pad 2 coated with fenpropathrin monoclonal antibody-colloidal gold marker;

[0027] b) preparing a reaction membrane 3 with a detection line 5 coated with a fenpropathrin hapten-carrier protein conjugate and a quality control line 6 coated with a goat anti-mouse anti-antibody;

[0028] c) Assemble the conjugate release pad 2 prepared in a) and b), the reaction membrane 3 , the sample absorbent pad 1 , the water absorbent pad 4 and the bottom plate 7 into a test paper.

[0029] The following step-by-step detailed description:

[0030] 1. Preparation of fenpropathrin hapten, see the synthetic route figure 1

[0031] 1) Synthesis of m-phenoxy cyanohydrin

[0032] Dissolve 1.22g m-hydroxybenzaldehyde in 50mL tetrahydrofuran, slowly add 0.65g potassium cyanide and 0.76mL d...

Embodiment 2

[0065] The detection of fenpropathrin residue in the tea sample of embodiment 2

[0066] 1. Pretreatment of tea samples

[0067] 1) The sample should be returned to room temperature 20-25°C before testing;

[0068] 2) Weigh 2.0±0.05g sample into a polystyrene centrifuge tube;

[0069] 3) Add 10mL of sample extract and mix well with a vortex instrument;

[0070] 4) The dilution method of green tea and dry tea is as follows:

[0071] Green tea: 100μL sample solution + 400μL sample diluent;

[0072] Dry tea: 100μL sample solution + 900μL sample diluent;

[0073] The sample diluent is 0.2moL / L phosphate buffer.

[0074] 2, detect with the test paper of embodiment 1

[0075] Use a straw to absorb the tea sample solution to be tested and add 2 to 3 drops vertically to the sample hole. When the liquid flows, start timing, react for 5 minutes, and judge the result. Other times are invalid.

[0076] 3. Analyze the test results, see Figure 5

[0077] Negative (-): Both the T l...

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Abstract

The invention discloses a test paper used for detecting fenpropathrin, and applications thereof. The test paper used for detecting fenpropathrin comprises a sample absorption pad (1), a combined compound release pad (2), a reaction membrane (3), a water absorption pad (4), and a base plate (7); the sample absorption pad (1), the combined compound release pad (2), the reaction membrane (3), and the water absorption pad (4) are glued onto the base plate (7) successively; the reaction membrane (3) is provided with a fenpropathrin hapten-carrier protein conjugate coated detection line (5), and a goat anti-mouse antibody coated reference line (6); the combined compound release pad (2) is coated with a fenpropathrin monoclonal antibody-colloidal gold marker. The test paper can be used for detecting residue fenpropathrin in tea, operation is simple, sensitivity is high, detection time is short, cost is low, and the test paper is suitable for screening and on-site monitoring of a large amount of samples.

Description

technical field [0001] The invention belongs to the technical field of rapid detection, and in particular relates to a test paper for detecting fenpropathrin, which is especially suitable for detecting fenpropathrin in tea samples. Background technique [0002] Fenpropathrin, α-cyano-phenoxybenzyl-2,2,3,3-tetramethylcyclopropane carboxylate, also known as fenpropathrin, is a kind of pyrethroids Insecticide and acaricide. Fenpropathrin is a nerve agent that can act on the nervous system of insects, causing them to be overexcited, paralyzed and dead. In recent years, pyrethroid pesticides have been widely used due to their high efficiency in killing insects. Among them, fenpropathrin has been detected in food, and fenpropathrin is moderately toxic to higher animals. [0003] At present, the detection of fenpropathrin residues is mainly carried out by liquid chromatography, gas chromatography and gas chromatography-mass spectrometry. The instrument method has the advantages o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N33/558G01N33/531
CPCG01N33/531G01N33/558G01N33/577G01N2430/12Y02A50/30
Inventor 冯才伟汪善良扶胜崔海峰冯静徐念琴杨梅袁学伟王江政袁光宇罗维超徐明慧杨昌松冉茂乾王吉平
Owner 贵州勤邦食品安全科学技术有限公司
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