Method of utilizing broad beans to evaluate ecological toxicity of residual atrazine in soil

An ecotoxicity and atrazine technology, applied in soil testing, measuring devices, instruments, etc., can solve the problem of less evaluation of soil organic pollutants

Active Publication Date: 2017-07-14
ANHUI UNIVERSITY OF ARCHITECTURE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Enzymes are widely used as biological indicators to evaluate the pollution degree of heavy metals to soil, but there are few evaluations on soil organic pollutants

Method used

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  • Method of utilizing broad beans to evaluate ecological toxicity of residual atrazine in soil
  • Method of utilizing broad beans to evaluate ecological toxicity of residual atrazine in soil

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1: A method for evaluating the ecotoxicity of soil residual atrazine using broad bean

[0023] A method for evaluating the ecotoxicity of soil residual atrazine by applying broad beans, including soil treatment and broad bean pot experiments; the soil treatment refers to adjusting the moisture content of the soil to be tested and the standard soil moisture content to 60wt%, and putting it in 25 ℃ 24 hours in the incubator; Described broad bean pot experiment comprises the following steps:

[0024] The first step: germination

[0025] The broad bean seeds are sterilized with 15% hydrogen peroxide for 20 minutes, then soaked in distilled water, and then transferred to an incubator to cultivate the broad bean seeds to germinate to obtain the seeds after germination;

[0026] Step Two: Germinate

[0027] The seeds after the germination are sown respectively in the second container containing the first container of the soil to be tested and the standard soil, and t...

Embodiment 2

[0032] Example 2: A method for evaluating the ecotoxicity of soil residual atrazine by applying broad bean

[0033] A method for evaluating the ecotoxicity of soil residual atrazine by applying broad beans, including soil treatment and broad bean pot experiments; the soil treatment refers to adjusting the moisture content of the soil to be tested and the standard soil moisture content to 60wt%, and putting it in 25 ℃ 24 hours in the incubator; Described broad bean pot experiment comprises the following steps:

[0034] The first step: germination

[0035] The broad bean seeds are sterilized with 15% hydrogen peroxide for 20 minutes, then soaked in distilled water, and then transferred to an incubator to cultivate the broad bean seeds to germinate to obtain the seeds after germination;

[0036] Step Two: Germinate

[0037] The seeds after the germination are sown respectively in the second container containing the first container of the soil to be tested and the standard soil,...

Embodiment 3

[0042] Example 3: A method for evaluating the ecotoxicity of soil residual atrazine by applying broad bean

[0043] A method for evaluating the ecotoxicity of soil residual atrazine by applying broad beans, including soil treatment and broad bean pot experiments; the soil treatment refers to adjusting the moisture content of the soil to be tested and the standard soil moisture content to 60wt%, and putting it in 25 ℃ 24 hours in the incubator; Described broad bean pot experiment comprises the following steps:

[0044] The first step: germination

[0045] The broad bean seeds are sterilized with 15% hydrogen peroxide for 20 minutes, then soaked in distilled water, and then transferred to an incubator to cultivate the broad bean seeds to germinate to obtain the seeds after germination;

[0046] Step Two: Germinate

[0047] The seeds after the germination are sown respectively in the second container containing the first container of the soil to be tested and the standard soil,...

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PUM

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Abstract

The invention discloses a method of utilizing broad beans to evaluate the ecological toxicity of residual atrazine in soil. The method comprises a soil treatment and a broad bean pot experiment. In the soil treatment, when the water content of soil to be detected and the water content of standard soil reach 60 wt%, the soil to be detected and the standard soil are stored in an incubator with a temperature of 25 DEG C for 20 to 30 hours. The broad bean pot experiment comprises steps of germination acceleration, germination, growth, and detection. The growth and physiological features of broad bean are taken as the indexes to effectively measure the environment pollution degree of atrazine.

Description

technical field [0001] The invention relates to a method for evaluating the ecological toxicity of soil residual atrazine by using broad beans, and belongs to the technical field of soil protection. Background technique [0002] Atrazine is widely used in farmland weeding and is the main herbicide in corn fields. According to statistics, more than 80 countries around the world are using atrazine to control weeds. The unscientific use of atrazine has resulted in a large amount of atrazine remaining in the farmland. Qiao Xiongwu et al. found that atrazine used in the field can be degraded by 90% in the same year, but the residual atrazine can still be detected after 130 weeks of application. Residual atrazine will harm the growth of crops and reduce the yield of crops. In 1993, in Zhangjiakou, Hebei, a large area of ​​rice was killed due to residual atrazine, and the total affected area reached 100,000 mu. For this reason, many scientific research institutions study the saf...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/24
CPCG01N33/24G01N2033/245
Inventor 杨英朱光蕤徐玲冯艳辉胡振东赵庆王健伟
Owner ANHUI UNIVERSITY OF ARCHITECTURE
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