A kind of method that green Sclerotinia oryzae produces five kinds of rice koji toxins

Abstract

The invention relates to a method for producing five kinds of rice smut toxins by Chlorosclerotinum oryzae, which comprises the steps of extracting toxins from rice culture medium cultured for 15 days. The green Sclerotinia sativa is Ustilaginoidea virens ZZY-2, which was obtained by separating and purifying the collection source, and was sent to the Chinese Typical Culture Center in Wuhan University, Wuhan City, Hubei Province on April 26, 2016. It is deposited in the Center for Material Conservation (CCTCC), and the accession number is CCTCC NO: M2016230.

Description

technical field [0001] The invention relates to a green sclerotinia oryzae, in particular to a method for the green sclerotinia oryzae to produce five kinds of aspergillus toxins. Background technique [0002] Rice false smut is a fungal disease of rice, which is caused by the infection of the panicle of rice by Ustilaginodea virens (Cooke) Takahashi. Rice false smut is relatively common and occurs in many countries such as China, Japan, the United States, India, and the Philippines, and is a worldwide rice disease (Fu et al, 2015; Ashizawa et al, 2010; Hamed et al, 2014). Before the 1980s, rice false smut was only sporadically distributed in middle and late rice fields, and it was a secondary disease of rice; however, with changes in rice varieties, farming methods, fertilizer dosage, and climate and environment, in recent years, rice false smut has changed from the past The sporadic distribution of rice has transformed into one of the major diseases in rice production (Wa...

AI Technical Summary

Problems solved by technology

In the chlamydospore suspension method, rice koji balls can be prepared into chlamydospore suspensions after being washed with sterile water. Since the surface layer of rice koji balls is accompanied by a large number of other pathogenic bacteria, their growth rate is faster than that of rice koji bacteria. It is easy to contaminate rice smut bacteria, and the miscellaneous bacteria that grow too fast cover a small amount of steamed bun-shaped rice smut bacteria colonies, which increases the difficulty of separation and purification

The disadvantage of the rice ball method is that a variety of pathogenic bacteria are associated with the rice ball, and they can also grow rapidly during the cultivation process and contaminate the target bacteria.

The sclerotia germination method is relatively easy to succeed. Studies have proved that sclerotia is the first choice for the efficient isolation of rice smut. However, the formation of sclerotia is limited by environmental conditions, and it is difficult to collect and obtain sclerotia. Moreover, it is rare in southern rice areas. This method is used to isolate southern rice Aspergillus is not effective

Spore shake-off method, because the surface of rice koji is not disinfected, a large number of bacteria on the surface of rice koji are easy to grow rapidly on the surface of the medium, which affects the germination, growth and purification of chlamydospores, and is as susceptible as the chlamydospore suspension method. Contaminated by other pathogens, it is not easy to successfully obtain strains of rice false smut

[0004] Therefore, only one type of toxin can be obtained in the traditional C. oryzae

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