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Method for cultivating micro capsule alga of producing microcysin in high yield

A technology of Microcystis and Microcystis aeruginosa, applied in the biological field, can solve the problems of complex extraction process, unstable toxicity, and difficulty in harvesting, and achieve the effect of simple extraction process, low cost, and high toxin production

Inactive Publication Date: 2006-01-04
INST OF AQUATIC LIFE ACAD SINICA
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Problems solved by technology

[0005] Although the toxic algae produced by water blooms are easy to obtain, they have many impurities, unstable toxicity, complicated extraction process and extremely expensive cost
The artificial cultivation of high-toxin-producing Microcystis in large quantities is an important prerequisite for the extraction of high-purity toxin standard products, because the cultured algae have few impurities, single toxin isomerization, stable toxicity, simple extraction process, and low cost, but the large-scale cultivation of Microcystis is easy. polluted, hard to harvest

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  • Method for cultivating micro capsule alga of producing microcysin in high yield

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Embodiment Construction

[0036] The steps of the present invention are as follows:

[0037] 1. Separation and purification of Microcystis aeruginosa: collect Microcystis from Dianchi Lake in Yunnan, suck out Microcystis under a microscope with a capillary tube, separate, purify and cultivate, compare the toxin production of Microcystin, and select the one with the highest toxin production Pure algae strains are used as the original species for mass cultivation.

[0038] 2. Screening of media: According to literature reports, the better media for cultivating Microcystis include MA, BG11 sudo M / 2# and modified Allen-Arnon commonly used by cyanobacteria. We cultured four strains of Microcystis aeruginosa FACHB905, FACHB942, FACHB915 and FACHB912 with these four mediums, and the experimental results showed that BG11>MA>Sudo M / 2#>Allen-Arnon. Microcystis was further cultured with BG11 and MA, and it was found that the algae of BG11 started better than that of MA, but the algae of MA were blue-green and la...

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Abstract

A method for highly cultivating poison microcapsule aglae includes collecting microcapsule algae selecting culture medium, optimizing culture condition to have the best way for growth of the microcapsule algae, using breeder seed for culture in box then expanding culture step by step and finally confirming optimum time for harvesting.

Description

technical field [0001] The invention relates to the field of biotechnology, and more specifically relates to a method for cultivating high-toxin-producing Microcystis. Background technique [0002] The harm of cyanobacterial bloom pollution and the toxins produced by its metabolism to the environment and human health has become one of the major environmental problems of global concern. Algal toxins affect human health through drinking water and the food chain. Long-term exposure can damage the liver and may induce liver cancer. The World Health Organization (WHO) and the United Nations Educational, Scientific, Cultural and Health Organization (UNESCO) have shown great concern about this issue. In the past ten years, the degree of eutrophication in many water bodies in my country has intensified, and the frequency and intensity of cyanobacterial blooms have gradually increased. The potential and direct harm of algal toxins in algae blooms to human h...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/12
Inventor 宋立荣刘梅甘南琴李林陈伟李阔宇
Owner INST OF AQUATIC LIFE ACAD SINICA
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