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Application of poria cortex triterpenoids in the preparation of medicines for treating nephropathy

The technology of Poria cocos skin and kidney disease is applied in the application field of Poria cocos triterpenoids in the preparation of medicines for treating kidney disease, which can solve the problem of high toxicity of traditional Chinese medicine extracts, and achieve the effects of reducing urine protein content and reducing the degree of lesions.

Active Publication Date: 2020-02-07
SHANDONG ACAD OF CHINESE MEDICINE
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] Aiming at the problem that the effect of current nephropathy treatment agents on some cases is still unsatisfactory, and the toxicity of traditional Chinese medicine extracts is high, the present invention provides a relatively safe and effective application of poria cocos triterpenoids in the preparation of medicaments for treating nephropathy

Method used

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  • Application of poria cortex triterpenoids in the preparation of medicines for treating nephropathy
  • Application of poria cortex triterpenoids in the preparation of medicines for treating nephropathy
  • Application of poria cortex triterpenoids in the preparation of medicines for treating nephropathy

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] 实施例1 茯苓皮三萜的提取制备。

[0025] (1)将茯苓皮用质量分数为1.5%的盐酸冲洗,然后用水冲洗至中性,晾干;

[0026] (2)将茯苓皮用体积分数为70%的乙醇溶液回流提取2次,每次提取时间为0.5h,茯苓皮与乙醇溶液的用量为1:10(g / ml),合并提取液,提取液回收乙醇至干;

[0027] (3)将步骤(1)的干物质用水溶解成溶液,加入体积比为7:1.5的正丁醇和乙酸乙酯的混合物萃取,正丁醇和乙酸乙酯的混合物的体积为水溶液的2倍,有机相回收溶剂至干,得粗品;

[0028] (4)粗品用100-120质量倍的乙醇溶解,配成溶液,所得溶液上中性氧化铝层析柱,中性氧化铝的质量为粗品的15倍,然后用乙醇和乙酸乙酯体积比为2:5的混合物进行洗脱,乙醇和乙酸乙酯混合物的用量为氧化铝的2倍,收集洗脱液;

[0029] (5)向洗脱液中加入活性炭,活性炭用量为粗品质量的3%,加热至沸腾并保持30min,趁热过滤,用乙醇洗涤活性炭,合并滤液及洗液,浓缩至原体积的1 / 3,静置沉淀、过滤、干燥,得茯苓皮三萜。

Embodiment 2

[0030] 实施例2 茯苓皮三萜中有效成分的测定。

[0031] 对提取物中羟基-羊毛甾7,9(11),24-三烯-21-酸和去氢依布里酸的含量进行测定,方法如下:

[0032] 仪器及试药:Waters高效液相色谱系统,Water600泵,996二极管阵列检测器,M32色谱工作站,Lichrospher-C18柱,200×4.6mm,5μ(江苏汉邦科技有限公司产品)。

[0033] 试剂:乙腈(色谱纯级),3β-羟基-羊毛甾7,9(11),24-三烯-21-酸和去氢依布里酸(山东省中药新型制剂中心制备,含量均达98.0 %above).

[0034] 检测条件:流动相:乙腈:0.5%乙酸水(80:20),检测波长242nm。

[0035] 对照品溶液的制备:精密称取3β-羟基-7,9(11),24-三烯-21-酸对照品,置10mL容量瓶中,甲醇稀释至刻度,制成228μg·mL -1 的对照品溶液;精密称取去氢依布里酸对照品,置10mL容量瓶中,甲醇稀释至刻度,制成130μg·mL -1 的对照品溶液。

[0036] 供试品溶液的制备:取实施例1中茯苓皮三萜约40mg,精密称定,置100mL容量瓶中,甲醇溶解并稀释至刻度,摇匀,用0.45μm的微孔滤膜过滤,取滤液,即得。

[0037] Determination method: Precisely draw 10 μL each of the above-mentioned reference substance solution and the test solution, and inject them into the liquid chromatograph respectively to obtain the chromatogram of the reference substance. figure 1 and extract chromatography figure 2 . According to peak area calculation, the content of 3β-hydroxy-lanoster 7,9(11), 24-triene-21-acid is 30.15%, the content of dehydroi...

Embodiment 3

[0038] Example 3 The efficacy of triterpenoids of poria cocos on doxorubicin nephrotic syndrome in rats.

[0039] 1 Materials and methods

[0040] 1.1 Instruments and medicines

[0041] WFZ UV-PC ultraviolet spectrophotometer (Unocal (Shanghai) Instrument Co., Ltd.); DXL-DL metabolic cage: Jiangsu Feng's Experimental Animal Equipment Co., Ltd.; calf serum albumin: Xi'an Walson Biotechnology Co., Ltd. Company; Coomassie Brilliant Blue G-250: China Pharmaceutical Group Shanghai Chemical Reagent Company; urine protein test paper: Guangdong Huadu Gaoerbao Biotechnology Co., Ltd.; doxorubicin hydrochloride: Zhejiang Hisun Pharmaceutical Co., Ltd., 10mg / bottle , batch number 130201, when used as a solvent, inject physiological saline as a solvent, and prepare a 2mg / ml solution; Poria cocos triterpenes use the sample in Example 1, and use olive oil as a solvent, and prepare a 10 mg / ml solution.

[0042] 1.2 Animals

[0043] Select healthy male Wistar rats, weighing 158 ± 13g, prov...

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Abstract

In order to solve the problems that the existing drugs for treating nephropathy are still not ideal in effect for certain cases and high in toxicity of Chinese herb extracts, the invention provides application of triterpene, which is safer and remarkable in curative effect, from poria cocos peel in preparation of medicines for treating the nephropathy; the triterpene from the poria cocos peel is an ethanol extract of the poria cocos peel. The triterpene from the poria cocos peel can reduce the pathologic change degree of the kidney, reduce the content of urinary protein caused by the nephropathy, reduce creatinine and urea nitrogen and play a role in the treatment for the nephropathy.

Description

technical field [0001] 本发明属于医药制剂领域,涉及一种茯苓皮三萜在制备治疗肾病药物中的应用。 Background technique [0002] 近年来,随着环境污染加重、人们工作和生活节奏的加快、职业竞争加剧、饮食结构改变以及抗生素的滥用,慢性肾炎的发病率呈逐年上升趋势。调查结果显示,我国成年人中慢性肾病患病率为10.8%,据此估计,18岁以上人群中约有1.2亿慢性肾病患者。本病为慢性病,病程长,为害大,严重影响健康和工作,我国每年用于慢性肾炎医疗费用等经济损失,每年达数亿元,给社会生产和经济带来巨大损失。慢性肾炎是以蛋白尿、血尿、水肿及高血压为主要表现的疾病,其起病隐匿,病程迁延,不同病理类型的预后有着显著差异,大部分患者病情进展缓慢,但最终都将发展至肾衰竭,目前现代医学尚无特效治疗方法。目前在临床治疗中多以西医治疗为主,主要以激素与细胞毒药物为治疗药,激素具阳刚之性,易伤阴液,易致湿热;细胞毒药物可导致骨髓抑制、肝功能损伤,治疗效果欠佳,且副作用大。研究发现,中医药能显著缓解慢性肾炎患者的临床症状,提高患者的生活质量,改善尿蛋白定量、血尿、尿微量白蛋白等指标。 [0003] 肾病综合征(nephrotic syndrome,NS)是多种病因使肾脏受损而致的一组临床综合症,是肾小球疾病的常见表现。中医无此病名,按其临床表现可归属于“水肿”、“虚劳”、“腰痛”等范畴。西医治疗NS主要应用糖皮质激素、免疫抑制剂、对症支持等方法,虽疗效尚可,但疗程长、易复发、易产生激素依赖和毒副作用。实践证明,单独用激素或并用免疫抑制剂,对某些病例的效果仍不理想,复发率较高。此外,应用激素和免疫抑制剂致使免疫功能降低功能,降低而增加感染使本病复发。 [0004] 中药治疗肾病综合征历史悠久,中医将肾病综合征可分为4型,即肺脾肾阳虚型、肾气虚型、气阴两虚型、肝肾阴虚型。临床上常用于治疗此病的方药有:脾肾阳虚型可用济生肾气丸,肺肾气虚型可用香砂六君子汤,肝肾阴虚型可用知柏地黄汤,气阴两虚型可用参芪地黄汤,兼湿热用三仁汤、藿香左金汤,兼水湿用五皮饮合五苓散,兼湿浊用胃苓汤,兼血瘀用补阳还五汤,中药复方治疗取得一定效果。中药单味治疗肾病综合征主要有雷公藤,属于卫予科攀缘木植物雷公藤的根,具有祛风、除湿、杀虫功用,属于有毒药物。近年...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/575A61K36/076A61P13/12
CPCA61K31/575A61K36/076A61K2236/333A61K2236/35A61K2236/39A61K2236/55A61K2300/00
Inventor 闫雪生孙丹丹于蓓蓓刘瑾
Owner SHANDONG ACAD OF CHINESE MEDICINE
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