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A modified medical soybean protein bone adhesive and its preparation method

A soybean protein and bone adhesive technology, which is applied in surgical adhesives, applications, medical science and other directions, can solve the problems of low bonding strength of soybean protein adhesives, which have not been widely used, and achieve enhanced bonding strength. , to promote bone tissue regeneration, the effect of a wide range of sources

Active Publication Date: 2020-02-07
NANJING UNIV OF FINANCE & ECONOMICS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the low bond strength of soybean protein binders, it has not been widely used.

Method used

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  • A modified medical soybean protein bone adhesive and its preparation method
  • A modified medical soybean protein bone adhesive and its preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Example 1 Preparation of Modified Soy Protein Isolate Bone Adhesive

[0014] At room temperature, dissolve soybean protein isolate in 2 mL of phosphate buffer solution (pH=7.00, 0.05 mol / L) to make a 2% soybean protein isolate solution, add 0.88 g of ascorbic acid at the same time, and stir at 22 °C for reaction After 10 minutes, add 250~350 μL of tyrosinase solution (0.1mg / mL, 570U / mg), continue to stir and react at 22°C for 6~12 minutes, centrifuge, dialyze, and freeze-dry to obtain modified soybean protein isolate.

[0015] The addition amount of tyrosinase described in Table 1 and the time for continuing the reaction were respectively prepared soy protein isolate A~I.

[0016] Table 1 Conditions of tyrosinase modified soybean protein isolate and yield of dopa

[0017]

[0018] Sampling 20uL of the above soybean protein isolate A~I for analysis, repeated three times, adding 20uL HCl (2mol / L), 20uLNaOH (2mol / L), 20uL solution (15% sodium molybdate and 15% nitrous ...

Embodiment 2

[0020] Embodiment 2 Modified soybean protein isolate bone adhesive and the determination of its adhesive strength

[0021] The modified soybean protein isolate E obtained in Example 1 is dissolved in the phosphate buffer solution of pH=7.00, and the concentration shown in the following table 2 is prepared into a modified soybean protein isolate binder of 2 to 15%. Put it on the bone fracture, soak it in anhydrous calcium chloride for 15 minutes after fixing, and fix it in an incubator at 25°C for 48 hours, then measure its adhesive strength on a universal testing machine and compare it with unmodified soybean protein isolate , and the test results are shown in Table 2 below.

[0022] Table 2 Adhesive strength of soy protein isolate bone adhesive

[0023]

[0024] It can be seen from Table 2 that the bonding strength of the tyrosinase-modified enhanced soybean protein isolate bone adhesive is the most obvious.

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Abstract

The invention relates to a modified medical soybean protein bone adhesive, and a preparation method thereof. The preparation method comprises following steps: soybean protein isolate is dissolved in a phosphate buffered solution, ascorbic acid is added, an obtained mixture is stirred for 10min of reaction at 22 DEG C, 250 to 350<mu>L of a tyrosinase solution is added, an obtained mixed material is stirred continuously for 6 to 12min of reaction, and at last, centrifugation, dialysis, and freeze drying are carried out so as to obtain modified soybean protein isolate; the obtained improved soybean protein isolate is dissolved in the phosphate buffered solution so as to prepare the 2 to 15% modified medical soybean protein bone adhesive. In applications, the modified medical soybean protein bone adhesive is used for coating broken bone for bonding. The modified medical soybean protein bone adhesive is high in adhesive strength and biocompatibility, and is capable of promoting bone regeneration.

Description

technical field [0001] The invention relates to the technical field of biomedical materials, in particular to a modified medical soybean protein isolate bone adhesive with high adhesive strength and good biocompatibility and a preparation method thereof. Background technique [0002] Medical adhesives refer to additives that can polymerize in the body to quickly bond between tissues or between tissues and non-tissues. They are mainly used for local adhesion and repair of organs or tissues, auxiliary hemostasis for traditional sutures, and bone or joint joints. Combination and positioning and other technical fields. The fibrin adhesives that have been marketed and applied clinically are mainly extracted and prepared. Fibrin adhesive has the advantages of biocompatibility, good plasticity, easy tissue absorption, no cytotoxicity, and convenient preparation. However, there are also shortcomings such as too small bonding strength and possible virus infection. With the develop...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L24/10A61L24/00
CPCA61L24/001A61L24/0042A61L24/108C08L89/00
Inventor 沈新春莫家露冯晨俞凌孔令艳汤晓智周建新
Owner NANJING UNIV OF FINANCE & ECONOMICS
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