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Seedling hardening and transplanting method for hybrid test-tube plantlet

A technology of test-tube seedlings and hybrids, which is applied in the field of plant engineering, can solve the problems of wasting time and labor, and achieve the effects of improving survival rate and saving time and labor costs

Inactive Publication Date: 2017-08-08
GRASSLAND RES INST OF CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the hardening and transplanting methods of hybrid test-tube plantlets make the survival rate of test-tube plantlets still remain at a low level, which wastes time and labor. rate method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0070] The seedling hardening method of embodiment 1 hybrid test-tube plantlet

[0071] Select the one that grows to a height of 4-6cm in a triangular flask and has at least 3 adventitious roots with a length of more than 2cm, the hybrid of Caucasian clover and white clover F 1 For the generation of test-tube seedlings, the sealing film of the triangular flask was punctured, and the culture was continued in the culture room until the surface of the medium in the triangular flask became turbid.

[0072] Among them, the temperature in the cultivation room is kept as follows: 28°C in the light period, 26°C in the dark period, and 90% humidity, the photoperiod in the culture room is 12h / d, and the light intensity in the culture room is 20000μmol / m 2 ·s.

Embodiment 2

[0073] The seedling hardening method of embodiment 2 hybrid test-tube plantlets

[0074] Select the one that grows to a height of 4-6cm in a triangular flask and has at least 3 adventitious roots with a length of more than 2cm, the hybrid of Caucasian clover and white clover F 1 For the generation of test-tube seedlings, the sealing film of the triangular flask was punctured, and the culture was continued in the culture room until the surface of the medium in the triangular flask became turbid.

[0075] Among them, the temperature in the culture room is kept as follows: 27°C in the light period, 25°C in the dark period, and 90% humidity, the photoperiod in the culture room is 12h / d, and the light intensity in the culture room is 20000μmol / m 2 ·s.

Embodiment 3

[0076] The seedling hardening method of embodiment 3 hybrid test-tube plantlets

[0077] Select the one that grows to a height of 4-6cm in a triangular flask and has at least 3 adventitious roots with a length of more than 2cm, the hybrid of Caucasian clover and white clover F 1 For the generation of test-tube seedlings, the sealing film of the triangular flask was punctured, and the culture was continued in the culture room until the surface of the medium in the triangular flask became turbid.

[0078] Among them, the temperature in the cultivation room is kept as follows: 28°C in the light period, 26°C in the dark period, and 80% humidity, the photoperiod in the cultivation room is 12h / d, and the light intensity in the cultivation room is 20000μmol / m 2 ·s.

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Abstract

The invention provides a seedling hardening method and a seedling transplanting method for a hybrid test-tube plantlet, and relates to the technical field of plant engineering. The seedling hardening method comprises:culturing a hybrid test-tube plantlet in an aseptic culture vessel until the test-tube plantlet grows to the height of 4-6 mm and grows at least three adventitious roots, breaking the aseptic environment of the culture vessel, enabling the test-tube plantlet to continue to grow until the surface of a culture medium in the culture vessel becomes turbid, and finishing seedlinghardening. The transplanting method comprises:taking out the hybrid test-tube plantlet from the culture vessel together with the culture medium, cleaning the culture medium at the root part with clean water, planting in a sterilized growth matrix, setting different conditions in a culture room according to different growth stages, and continuing to culture. The provided seedling hardening method and the seedling transplanting method for the hybrid test-tube plantlet effectively improve the survival rate of the hybrid test-tube plantlet, and save the time cost and the labor cost.

Description

technical field [0001] The invention relates to the technical field of plant engineering, in particular to a method for hardening and transplanting hybrid test-tube seedlings. Background technique [0002] Hybridization between different species, genus or species with more distant relationship in plant taxonomy is usually called distant hybridization. As a breeding method, distant hybridization breaks the boundaries between species, artificially promotes the exchange and combination of genetic material between different species, and combines the unique traits of different biological types into a common hybrid individual to varying degrees in order to create new species. Therefore, it plays an important role in the research of plant genetics and breeding, and has received long-term attention from genetic and breeding experts. Its application in breeding mainly includes the artificial creation of new species, the improvement of stress resistance of varieties, the creation of...

Claims

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Application Information

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IPC IPC(8): A01G9/10
CPCA01G9/029Y02A40/25Y02P60/14
Inventor 黄帆李俊刘磊李志勇李鸿雁师文贵解永凤
Owner GRASSLAND RES INST OF CHINESE ACAD OF AGRI SCI
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