Method for measuring plant hormone in subgenus yulania plant

A plant hormone and plant technology, applied in the field of biochemistry, can solve the problems of unsatisfactory pigment removal effect, emulsification of the extract, inability to stratify, etc., and achieve the effect of good peak shape, high resolution, and no tailing

Active Publication Date: 2017-08-08
BEIJING FORESTRY UNIVERSITY
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] The second technical problem to be solved by the present invention is to overcome the problem of unsatisfactory pigment removal effect, the problem that the extract is emulsified and cannot be separated in the pretreatment method of magnolia subgenus, especially the safflower magnolia or white magnolia. At least one problem in plant hormone ionization

Method used

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  • Method for measuring plant hormone in subgenus yulania plant
  • Method for measuring plant hormone in subgenus yulania plant
  • Method for measuring plant hormone in subgenus yulania plant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] In this example, the following instruments and reagents were used.

[0064] Instrument: American Agilent 1260 high performance liquid chromatography, including quaternary pump, automatic sampler, column thermostat and ultraviolet detector; 3-30K high-speed refrigerated centrifuge (Germany SIGMA); Hei-VAP rotary evaporator (Germany Heidolph ); PB-21 acidity meter (Sartorius, Germany); Quintix 224-1CN electronic balance (Sartorius, Germany); pipette (Eppendorf, Germany); SHB-Ⅲ circulating water multi-purpose vacuum pump (Zhengzhou Great Wall Science, Industry and Trade Co., Ltd.).

[0065] Reagents: polyvinylpolypyrrolidone (PVPP) (Sigma, USA); methanol (chromatographically pure, Fischer Scientific, USA); glacial acetic acid, ethyl acetate, ammonia water, hydrochloric acid (analytical grade, Beijing Chemical Plant); ultrapure water; GA3, IAA and ABA standard (HPLC grade, purity ≥ 98%, Sigma, USA).

[0066] The method for measuring phytohormones in plants of the subgenus ...

Embodiment 2

[0097] The safflower magnolia plant tissue in embodiment 1, that is, the safflower magnolia flower buds are replaced with safflower magnolia tepals, safflower magnolia leaves, white magnolia tepals and white magnolia leaves respectively, and other steps are the same, and the phytohormone determination is carried out repeatedly. Sample 3 times. The GA3, IAA, and ABA contents of each plant tissue are shown in Table 3, and the GA3 content of the flower buds of Magnolia safflower is the highest, which is 125.31±0.43μg / g.FW; Except for ABA, the other two hormones were lower than those of the magnolia perianth in full bloom; the sampling period of magnolia safflower leaves was summer, and the contents of each hormone were higher; the sampling period of magnolia leaves was autumn, and the IAA content The lowest was 0.84±0.04μg / g.FW.

[0098] Table 3 Hormone content in different plant tissues

[0099]

[0100] *Mean±SD.

[0101] The results showed that this method was suitable f...

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Abstract

The invention discloses a method for measuring plant hormone in a subgenus yulania plant. The method comprises a plant hormone extraction step, a standard solution preparing step, a high performance liquid chromatogrophy step and a standard solution measuring step, wherein in the plant hormone extraction step, low-temperature grinding, low-temperature leaching at a dark place, PVPP (Crosslinking Polyvingypyrrolidone) extraction and the like are adopted, so that efficient extraction of zeatin is ensured. The method has the advantages of better peak form, high separation degree, no tailing phenomenon, high repeatability, high recycling rate, accuracy and reliability.

Description

technical field [0001] The invention belongs to the field of biochemistry, and relates to qualitative and quantitative analysis techniques of plant hormones, in particular to a method for measuring endogenous hormones in plants of the subgenus Magnolia, particularly gibberellin (GA3), gibberellin (GA3), Determination methods of auxin (IAA) and abscisic acid (ABA). Background technique [0002] Safflower Magnolia (Magnolia wufengensis L.Y.Ma et L.R.Wang) is a new species of Magnolia subgenus Magnolia discovered in Wufeng, Hubei in March 2004. Uniformity of flower color is very rare. Subsequent surveys in this area found wild species with 12, 15, 18, 24, and even as many as 46 perianth segments of magnolia magnolia, which will be similar in flower color to safflower magnolia and increase in the number of perianth segments Officially named Magnolia wufengensis var. multitepala L.Y.Ma et L.R.Wang. With the continuous advancement of the breeding and breeding of new varieties o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 马履一肖爱华段劼贾忠奎汪力陈发菊桑子阳张德春陈思雨赵潇梁爽
Owner BEIJING FORESTRY UNIVERSITY
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