Application of human-derived monocytic series U937 in preparation of anti-influenza virus and drug screening model of inflammation factor induced by virus

Abstract

The invention discloses application of a human-derived monocytic series U937 in preparation of an anti-influenza virus and a drug screening model of an inflammation factor induced by the virus. In the application, an influenza virus can effectively infect a U937 cell line and can induce a series of important inflammatory factor expressions including MCP-1, IP-10, IL-8, IL-6 and the like, and the toxicity and antiviral and anti-inflammatory activity of drugs can be comprehensively evaluated in a round of experiments by detecting the activity of injected cells, the virus level in supernatant and the level of relevant inflammatory factors. The established cell screening model is accurate, efficient, low in cost, short in consumed time and free of deviation property and can be used for high throughput screening of antiviral and anti-inflammatory drugs for treating the influenza virus.

Description

technical field [0001] The invention belongs to the technical field of medicine, and mainly relates to the application of human mononuclear cell line U937 in preparing a drug screening model for resisting influenza virus or its induced inflammatory factors. Background technique [0002] Influenza virus infection seriously threatens human health and brings huge economic losses to society. Previous studies have shown that after human infection with highly pathogenic influenza virus, it will cause extensive necrosis of bronchi and bronchiole cells, accompanied by shedding of ciliated epithelial cells, fibrin exudation, and inflammatory cell infiltration, causing acute lung injury. At the same time, in cases of severe influenza infection, it is generally found that there is a "cytokine storm", that is, the expression of a large number of inflammatory factors is up-regulated due to virus infection. These inflammatory factors are mainly expressed by leukocytes such as monocytes / m...

AI Technical Summary

Problems solved by technology

However, the current cell screening model still has certain deficiencies in the screening of anti-influenza drugs: first, the model detection index is single

The screening model of anti-influenza virus itself can only detect antiviral drugs, while the anti-inflammatory screening model can only detect anti-inflammatory drugs, and cannot systematically evaluate the comprehensive effect of drugs on influenza and its induced inflammation

Second, the sensitivity is not high

Some traditional inflammatory cell models are not sensitive to influenza; and some influenza-sensitive cell lines, such as MDCK cell lines (canine kidney cell lines), have little clinical reference value due to the gap between them and human cells

Third, the pertinence is not strong

Some traditional inflammatory cell models, the indicators that can be detected have little effect on influenza-induced inflammation

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