Lactobacillus plantarum and application thereof to silage preparation

A technology of Lactobacillus plantarum and silage, applied in the field of Lactobacillus plantarum and its application in the preparation of silage, can solve the problems of slow growth of lactic acid bacteria, low acid production, and low quality of silage, and achieve accelerated fermentation process and low cost , the effect of lowering the pH value

Inactive Publication Date: 2017-08-18
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to overcome the problems of low silage quality due to slow growth of lactic acid bacteria and low acid production in the silage preparation process in the prior art, and propose a method that can quickly reduce the pH value, speed up the fermentation process, and effectively improve the quality of alfalfa silage. Lactobacillus plantarum

Method used

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  • Lactobacillus plantarum and application thereof to silage preparation
  • Lactobacillus plantarum and application thereof to silage preparation
  • Lactobacillus plantarum and application thereof to silage preparation

Examples

Experimental program
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Effect test

Embodiment 1

[0033] Example 1: Isolation and strain identification of Lactobacillus plantarum CAU-a214

[0034] 1. Isolation of strains

[0035] Separation of Lactobacillus plantarum by dilution plate method: add 10 g of alfalfa silage 60-day samples to 90 mL of sterilized distilled water to shake evenly, dilute 10 times in a gradient, and then take 10 -1 、10 -3 and 10 -5 20 μL of each sample dilution was spread on the MRS medium, and incubated in a 30°C constant temperature incubator for 48 hours; according to the characteristics of colony size, shape and color, typical colonies were picked, and catalase activity determination, Gram staining and Microscope test. All strains that are Gram-positive and catalase-negative can be initially identified as Lactobacillus, and they are streaked and purified twice on MRS medium, and then stored in 1ml of NB cultured with 10% DMSO stored in a -80°C ultra-low temperature freezer.

[0036] MRS medium: 10.0g of peptone (Proteose peptone NO.3); 10.0...

Embodiment 2

[0066] Embodiment 2: the preparation of alfalfa silage

[0067] The preparation of alfalfa silage comprises the following steps:

[0068] (1) Dry the alfalfa to a moisture content of about 60%, cut it to 1cm-2cm, and mix well;

[0069] (2) To step (1) alfalfa according to 6log cfu g -1 Lactobacillus plantarum (Lactobacillus plantarum) CAU-a214 was inoculated, and no addition (Control), GFG (commercial bacterial agent), FG1 (commercial bacterial agent) were used as controls, and each treatment was repeated 3 times;

[0070] (3) Put the alfalfa from step (2) into polyethylene silage bags of 30cm×20cm, and process three bags for each bag, with 100g in each bag, pump air with a vacuum sealing machine, seal, and store at room temperature for 60 days.

[0071] Nutrient components, fermentation quality, microbial analysis and in vitro digestion experiments were carried out after alfalfa silage for 60 days.

[0072] 1. Identification of nutritional components and fermentation quali...

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Abstract

The invention belongs to the field of microorganism application and feed compounding and processing and discloses a strain of lactobacillus plantarum and application thereof to silage preparation. A preservation number of lactobacillus plantarum CAU-a214 is CGMCC NO.13609 and can be used for preparation of silage especially alfalfa silage. The lactobacillus plantarum CAU-a214 has advantages that quickness in pH value decrease, acceleration of fermentation process and increase of ensiling rate can be realized; propagation of undesirable microorganisms can be inhibited so as to improve alfalfa silage quality, alfalfa nutrients can be better preserved, silage in-vitro digestibility is improved, and the problem of difficulty in silage fermentation is solved. Compared with existing lactobacillus additives on the market, additives prepared from lactobacillus plantarum CAU-a214 have advantages of excellent effects, low cost, safety and easiness in use.

Description

technical field [0001] The invention belongs to the field of microorganism application and feed preparation and processing, and in particular relates to a plant lactobacillus and its application in preparing silage. Background technique [0002] Alfalfa is the largest cultivated forage grass in my country. With the rapid development of animal husbandry in China and the continuous adjustment of the structure of rural planting industry, the large-scale planting and industrialization of alfalfa are developing rapidly. The alfalfa industry in my country is based on alfalfa hay, but rain or humidity can easily cause the loss of nutrients in the process of production and storage, which seriously limits the healthy development of the alfalfa industry. Therefore, it is necessary to find new ways to solve the problem of alfalfa processing and storage. Silage is an ideal measure to solve the above problems. Silage refers to a fresh-keeping technology that ferments green feed under se...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K30/18
Inventor 杨富裕李东霞张颖超姚丹丹玉柱
Owner CHINA AGRI UNIV
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