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Measurement method of porcine 2-cell embryo volume
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A measurement method and cell technology, which is applied in the field of measuring the volume of pig 2-cell embryos, can solve the problem of inaccurate estimation of the volume of pig 2-cell embryos, and achieve the effect of improving production efficiency and developmental potential
Inactive Publication Date: 2021-09-17
ANIMAL HUSBANDRY RES INST OF HEILONGJIANG ACADEMY OF AGRI SCI
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[0003] Existing methods of measurement are diameterestimation methods (eg figure 1 shown), but this method is not accurate enough to estimate the volume of porcine 2-cell embryos. In addition, after the same embryo is rotated by 90 degrees, the observed volume will change greatly (such as figure 2 shown)
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Embodiment 1
[0044] A method for measuring the volume of pig 2-cell embryos, comprising: pig 2-cells, the method comprising the steps of:
[0045] (1) Make a regression curve
[0046] A. Stain the 2-cell cleavage embryo, place the polar body at the 12 o'clock position of the embryo, take pictures under the microscope, use a rectangular frame to correct the edge of the measurement data and then measure the length, respectively get the left cell width LX1, the left cell length LY1, and the right cell length Cell width RX1, right cell length RY1;
[0047] B. Rotate 90 degrees along the direction of the cleavage groove, rotate the polar body to the top of the embryo, take the second photo, use a rectangular frame to correct the edge of the measurement data and then measure the length to obtain the left cell width LX2, the left cell length LY2, and the right cell Width RX2, right cell length RY2;
[0048] C. From the LX1, LX2, LY1, LY2, RX1, RX2, RY1, and RY2 measured in steps A and B, calcul...
Embodiment 2
[0055] According to the method for measuring the volume of pig 2-cell embryos described in Example 1, the embryo staining agent is Hoechst33342.
Embodiment 3
[0057] According to the method for measuring the volume of pig 2-cell embryos described in Example 1 or 2, in the steps A, B, and D, when taking pictures, focus on the pictures for five consecutive times to ensure that the maximum length is obtained.
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Abstract
A method for measuring the volume of porcine 2-cell embryos. Pig is an important economic animal. The research on its reproductive regulation can not only provide a reference for the study of human reproductive physiology and pathological process, but also provide a theoretical basis for improving the reproductive performance of pigs. a puzzle. A method for measuring the volume of pig 2-cell embryos. By photographing and measuring pig 2-cell embryos under a microscope, making a regression curve and obtaining a regression equation, a relatively accurate embryo volume can be obtained by simply photographing and measuring, which is beneficial to Further research to improve the developmental potential of pig embryos and improve pig production efficiency is a reliable method for identifying high-quality embryos. The invention is applied in the technical field of embryoengineering.
Description
Technical field: [0001] The invention relates to a method for measuring the volume of pig 2-cell embryos. Background technique: [0002] Pig is an important economic animal and a biomedical model animal. The research on its reproductive regulation can not only provide a reference for the study of human reproductive physiology and pathological process, but also provide a theoretical basis for improving the reproductive performance of pigs. However, low fecundity is a problem that plagues the pig industry in my country. Low fecundity is reflected in various stages, including low egg meiotic maturation rate, low early embryo development rate after fertilization, low pregnancy rate and low live offspring rate. Among them, the high rate loss during pig egg maturation and early embryo development has become an important problem. Therefore, improving the developmental potential of pig oocytes is of great significance for improving pig production efficiency. In order to improve th...
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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/02
CPCG01N33/5005
Inventor 刘娣郭镇华吕蕾吴宏达杨云春
Owner ANIMAL HUSBANDRY RES INST OF HEILONGJIANG ACADEMY OF AGRI SCI
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