Deproteinization and decoloration method for mango processing by-product polysaccharide
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A deproteinization and by-product technology, applied in the field of mango processing by-product polysaccharide deproteinization and decolorization, can solve the problems of optimization, no mango polysaccharide deproteinization and decolorization process, etc.
Active Publication Date: 2017-08-22
SOUTH SUBTROPICAL CROPS RES INST CHINESE ACAD OF TROPICAL AGRI SCI
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Problems solved by technology
But before that, there was almost no research on the optimization of deproteinization and decolorization process of mango polysaccharides
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Embodiment 1
[0065] Embodiment 1 adopts the following steps to realize the present invention:
[0066] (1) Pretreatment of by-products of mango processing: select undamaged and disease-free mangoes, take the pomace, wash, slice, inactivate in a boiling water bath for 2 min, mix mango pomace with Mixed with distilled water, homogenized in a masher to obtain a homogenized mango peel residue, refrigerated at 4°C for later use;
[0067](2) Extraction of mango crude polysaccharides: Take 30g of mango peel residue homogenate treated in step (1), add 90mL of distilled water, rehydrate for 30min, adjust pH to 5.0, add cellulase with 1.0% of mango peel residue mass, and use ultrasonic Use the power of 240W in the washing machine, extract at 50°C for 60min, heat up to 90°C to inactivate the enzyme for 30min; the obtained extract is centrifuged at 6000r / min for 10min, the supernatant is collected, and the residue is repeatedly extracted once; the extract is separated from the supernatant , concentra...
Embodiment 2
[0070] Embodiment 2 adopts the following steps to realize the present invention:
[0071] (1) Pretreatment of by-products of mango processing: select undamaged and disease-free mangoes, take the skin dregs, wash, slice, inactivate in a boiling water bath for 2 min, mix the mango skin dregs with the Mixed with distilled water, homogenized in a masher to obtain a homogenized mango peel residue, refrigerated at 4°C for later use;
[0072] (2) Extraction of mango crude polysaccharides: take 30 g of mango peel residue homogenate treated in step (1), add 120 mL of distilled water, rehydrate for 30 minutes, adjust pH to 5.0, add cellulase with 0.8% of mango peel residue mass, and use ultrasonic In the washing machine, extract at 50°C for 80min with a power of 300W, and then heat up to 90°C to inactivate the enzyme for 30min. The obtained extract was centrifuged at 5000r / min for 10min, the supernatant was collected, and the residue was repeatedly extracted once; the supernatant was s...
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decolorization rate
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Abstract
The invention discloses a deproteinization and decoloration method for a mango processing by-product polysaccharide. The method comprises the steps of pre-treating mango peel slag to prepare mango peel slag slurry; extracting crude polysaccharide by using an ultrasonic-assisted cellulose enzyme method; and carrying out deproteinization and decoloration on crude polysaccharide to obtain a mango polysaccharide semi-pure product. The crude polysaccharide is extracted by using the ultrasonic-assisted cellulose enzyme method, diffusional release of the polysaccharide can be accelerated by secondary vibration efficiency of an ultrasonic wave, the cell wall can be effectively broken through cellulose enzyme treatment, the yield of the polysaccharide is improved and the biological activity of the polysaccharide can be kept. A relatively good deproteinization effect can be obtained through deproteinization by adopting an optimized TCA-n-butyl alcohol method; and the deproteinized polysaccharide solution is decolored by adopting activated carbon, so that relatively high decoloration rate and polysaccharide retention rate can be obtained. The mango processing by-product is taken as a raw material, so that improvement of the additional value of the mango deep processing industry is facilitated and the environmental pollution is relived. The method is simple and fast; and compared with a traditional dry crushed sample pretreatment method, the extraction process is simplified and the production cost is reduced.
Description
technical field [0001] The invention belongs to the technical field of deep processing of horticultural crop products, and in particular relates to a method for deproteinizing and decolorizing the polysaccharide by-product of mango processing. Background technique [0002] Mango, also known as honey hope and lemon fruit, is a tropical evergreen tree of Anacardiaceae. It is widely planted in tropical and subtropical regions of my country. It is deeply loved by the public because of its delicate flesh, rich nutrition and unique flavor. Emollient and antiemetic effects. Every year, millions of tons of skin dregs are produced in the process of mango production and processing, which are often treated as garbage piles and seriously pollute the environment. [0003] Polysaccharide is one of the main active ingredients in mango pomace, which has anti-oxidation, antibacterial, anti-tumor, immune regulation, hypoglycemic, hypolipidemic and other effects. In recent years, the extracti...
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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
CPCC08B37/0003
Inventor 胡会刚赵巧丽谢江辉胡玉林陈晶晶段雅婕
Owner SOUTH SUBTROPICAL CROPS RES INST CHINESE ACAD OF TROPICAL AGRI SCI
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