Biomimetic immunosorbent detection method for trichlorfon based on quantum dots

A detection method and technology of quantum dots, which are applied in biological testing, measurement devices, material inspection products, etc., can solve the problems of expensive instruments, cumbersome pretreatment, long detection time, etc., and achieve low cost, high sensitivity, and quantum yield. high effect

Active Publication Date: 2019-02-12
SHANDONG AGRICULTURAL UNIVERSITY
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  • Claims
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Problems solved by technology

[0004] The purpose of the present invention is to overcome the shortcomings of the traditional trichlorfon detection method, such as low sensitivity, long detection time, expensive equipment, cumbersome pre-treatment, etc., and provide a bionic immunoadsorption detection method based on quantum dot-labeled trichlorfon

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  • Biomimetic immunosorbent detection method for trichlorfon based on quantum dots
  • Biomimetic immunosorbent detection method for trichlorfon based on quantum dots
  • Biomimetic immunosorbent detection method for trichlorfon based on quantum dots

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Embodiment Construction

[0021] Below in conjunction with embodiment, the present invention is further described; Following embodiment is illustrative, not limiting, can not limit protection scope of the present invention with following embodiment.

[0022] In the invention, the trichlorfon molecularly imprinted polymer film is used as a biomimetic antibody in conjunction with an enzyme-linked immunological technique, thereby establishing a rapid detection method with high sensitivity for the trichlorfon. Its specific embodiment is:

[0023] 1. Preparation of quantum dot-labeled antigen: mix 16 μL trichlorfon hapten (1 mM), 32 μL 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (10 mM) and 10 μL CdSe / ZnS Amino quantum dots (8 μM) were sequentially added to the reaction vessel and mixed thoroughly; 70 μL of borate buffer solution (pH 7.4) was added and reacted at room temperature in the dark for 2 h. After the reaction, centrifuge to remove aggregates, concentrate the reactant by ultrafiltration for sep...

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Abstract

The invention relates to a quick detection method of trichlorfon based on quantum dot labeling. The quick detection method is characterized by taking quantum dot labeled haptens as markers, taking a hydrophilic molecularly-imprinted polymer membrane as a bionic antibody, and utilizing an immunoadsorption measuring theory to establish the quick sensitive detection method with an extensive detection range on trichlorfon. The quick detection method combines the molecular imprinting technique and the immunity technique to establish the bionic immunoadsorption detection method with high sensitivity on trichlorfon, greatly shortens the analysis time (shortened by 1.0h compared with biological immunity analysis), and is suitable for quick detection of trichlorfon. The quick detection method is low in cost, high in sensitivity and simple in experiment operation, and can be extensively used in detection of trichlorfon in various foods.

Description

technical field [0001] The invention relates to the technical field of food safety detection, in particular to a bionic immunoadsorption detection method based on quantum dot-labeled trichlorfon. Background technique [0002] Trichlorphon (trichlorphon, O, O-dimethyl-(2,2,2-trichloro-1-hydroxyethyl) phosphonate) is a widely used organophosphorus spectrum insecticide with high efficiency, Low toxicity, low residue, good water solubility and other characteristics, so it is widely used in agricultural production. However, the unreasonable application of trichlorfon in the process of pesticide production also brings about the problem of pesticide residue hazards, and its damage to animal nervous system, carcinogenicity and mutagenicity have also attracted more and more attention. GB 16319-1996 stipulates that the maximum residue of trichlorfon in food shall not exceed 0.1mg Kg -1 . [0003] At present, most domestic and foreign detection methods for trichlorfon in food use ga...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/53G01N33/532
CPCG01N33/53G01N33/532
Inventor 徐志祥刘秋蕊姜名荻徐龙华
Owner SHANDONG AGRICULTURAL UNIVERSITY
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