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Formulations useful for photodynamic therapy or diagnosis

A technology for preparations and solvates, used in preparations containing diketone compounds, in the field of photodynamic therapy or diagnosis, can solve problems such as limited application and reduced photochemical efficiency, and achieve good safety, good bioavailability, and enhanced immunity. Effect

Pending Publication Date: 2022-05-17
BEIJING WHOLESOMETECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the existence of the large π-conjugated ring system of this type of photosensitizer, there is a strong π-π stacking interaction between the molecules, which leads to the aggregation of porphyrin and / or phthalocyanine molecules and reduces its photochemical efficiency. greatly limit its clinical application

Method used

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  • Formulations useful for photodynamic therapy or diagnosis
  • Formulations useful for photodynamic therapy or diagnosis
  • Formulations useful for photodynamic therapy or diagnosis

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0162] Experimental Example 1. Evaluation of Compound Toxicity to Tumor Cells

[0163] In this experimental example, the toxicity of the compound to tumor cells was detected by the MTT method. The specific steps are as follows:

[0164] (1) Collect the logarithmic phase cells, adjust the concentration of the cell suspension, add 100 μL to each well, and plate to adjust the density of the cells to be tested to 1000-10000 wells (the edge wells are filled with sterile PBS).

[0165] (2) 5%CO 2 and incubate at 37°C until the cell monolayer covers the bottom of the well (96-well flat-bottom plate), and then add the drug in a gradient concentration. Generally, there are 5-7 gradients, 100 μL per well, and 3-5 duplicate wells.

[0166] (3) The cells were randomly divided into light-administered and dark-administered groups, and 5% CO 2 , incubate at 37°C for 16-48 hours, and observe under an inverted microscope.

[0167] (4) Laser with a wavelength of 450nm-480nm (illumination in...

experiment example 2

[0182] Experimental example 2. Evaluation of the inhibitory effect of compounds on bacteria

[0183] A single colony of E. coli was transferred from a solid Luria Bertani (LB) agar plate to 5 ml of liquid LB medium and incubated at 37°C for 12 hours. Bacteria were collected by centrifugation (7000 rpm, 1 min) and washed three times with PBS buffer. Discard the supernatant and resuspend the remaining E. coli in PBS buffer. Adjust the optical density (OD600) of the bacterial suspension to 1.0. The suspension was then diluted (5-fold) with PBS buffer. Incubate the diluted E. coli cell suspension with a photosensitizer (diketone compound) solution at a concentration of 60 mM for 15 minutes at 37°C in the dark, and then at 15 mW / cm 2 Illuminate under laser light for 200s (wavelength 450nm-480nm, the final light dose is about 3J cm -2 ), the bacterial suspension after illumination was serially diluted with PBS buffer (10 4 times). Disperse 100 µL of the diluted bacterium E. co...

experiment example 3

[0188] Experimental example 3. Evaluation of compound's inhibitory effect on tumor (animal experiment)

[0189] 1. Using BJMU-204 as a photosensitizer, BALB / C mice were used to conduct photodynamic therapy tumor effect experiments.

[0190] Modeling method: BALB / C mice were divided into 5 groups, with 11-12 mice in each group. Resuscitate and subculture 4T1 (mouse breast cancer cells) until the cells are in a good state, and are used for tumor formation inoculation. BALB / C mice were depilated and sterilized locally, and tumor cells were injected into the mouse mammary gland fat pad in situ. Tumors formed in 7-10 days.

[0191] Dosing time: Drug intervention was given after the mice became tumors, and intratumoral injections were administered on the next day for photodynamic therapy (laser wavelength 450nm-480nm, light intensity 200mW / cm 2 ).

[0192] Administration method: see the table below, the photosensitizer was dissolved in normal saline, and the mice in groups 1-3 w...

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Abstract

The invention belongs to the technical field of medicines, and relates to a preparation for photodynamic therapy or diagnosis. Particularly, the invention relates to a preparation which takes a compound as shown in a formula (I), pharmaceutically acceptable salt or ester, a prodrug, a stereoisomer, a hydrate, a solvate, a crystal form, an isotope label, metabolite forms of the compound or any combination or mixture of the compound, the pharmaceutically acceptable salt or ester, the prodrug, the stereoisomer, the hydrate, the solvate, the crystal form and the isotope label as an active ingredient, the preparation is a liquid preparation. The formulations of the present invention can be used for photodynamic therapy or photodynamic diagnosis of diseases, or for beautifying the skin by photodynamic therapy.

Description

technical field [0001] The invention belongs to the technical field of medicine, and relates to a preparation containing a diketone compound and its application in photodynamic therapy or diagnosis. Background technique [0002] Photodynamic therapy (Photodynamic Therapy) is a kind of photochemotherapy, which is based on photosensitizer sensitization to induce oxygen to generate reactive oxygen species (ROS) represented by singlet oxygen, which can kill tumor cells or pathogenic microorganisms to achieve purpose of treating disease. Photodynamic therapy is a new type of therapy, which has been applied in some clinical treatments including tumors, genital warts, acne and port wine stains. Among them, compared with the traditional therapy for tumor treatment, photodynamic therapy has the obvious advantages of convenient operation, high selectivity and less side effects. In addition, photodynamic therapy can also be used for skin beauty. Photodynamic diagnosis refers to the ...

Claims

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Application Information

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IPC IPC(8): A61K9/08A61K31/12A61K31/121A61K31/192A61K31/341A61K31/444A61K41/00A61K47/10A61P15/02A61P17/00A61P17/10A61P27/02A61P31/04A61P31/10A61P31/12A61P33/02A61P33/14A61P35/00A61P37/02A01N35/02A01N35/04A01N37/42A01N43/40A01N43/08A01P1/00A01P3/00A01P15/00
CPCA61K9/08A61K47/10A61K31/121A61K31/192A61K31/12A61K31/444A61K31/341A61K41/0057A61P17/00A61P17/10A61P31/04A61P31/10A61P31/12A61P33/02A61P33/14A61P37/02A61P35/00A61P15/02A61P27/02A01N35/02A01N35/04A01N37/42A01N43/40A01N43/08A01P15/00A61K41/00A01P3/00A01P1/00A61P31/00A61P33/00Y02A50/30
Inventor 不公告发明人
Owner BEIJING WHOLESOMETECH CO LTD
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