Application of pedunculoside in preparation of anti-(hepatitis-B-virus) medicines
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A technology of pedicel glucoside and hepatitis B virus, which is applied in antiviral agents, drug combinations, and pharmaceutical formulas, etc.
Active Publication Date: 2017-08-29
SUN YAT SEN UNIV +1
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Embodiment 1
[0013] The preparation of embodiment 1 rescue must be medicinal material extract
[0014] Get an appropriate amount of Bing medicinal materials, crush them, pass through a No. 1 sieve, add 8 times the amount of 70% (volume fraction) ethanol to the medicinal materials, heat and reflux for extraction twice, each time for 1 hour, filter, combine the extracts, put them in a rotary evaporator, Concentrate under reduced pressure at 80°C to a thick paste. Transfer the concentrated liquid to a decompression drying oven, continue drying and crushing at 80°C, and obtain the extract of the rescued Bing medicinal material.
[0018] 1.2 Control drugs: lamivudine, oleanolic acid, extracts from medicinal materials of jiubing (see Example 1)
[0019] 1.3 In vitro cell model: 2.2.15 cell line (HepG2.2.15 cells) transfected with hepatitis B virus. Inoculate HepG2.2.15 cells in DMEM medium (containing 10% fetal bovine serum, 380 μg / ml G418, 0.03% glutamine, 100 μg / ml each of penicillin and streptomycin) in 5% CO 2 , cultured in a 37°C incubator.
[0020] The above drugs were fully dissolved in dimethyl sulfoxide (DMSO) and stored at -20°C for later use.
[0021] 2. Reagents and main instruments
[0022] Dimethyl sulfoxide (DMSO, AR), MTT (thiazolium blue, AR), hepatitis B virus surface antigen detection kit and hepatitis B virus e antigen detection kit were purchased from Shanghai Industrial Kehua Biotechnology Co., Ltd.
[0023] 3. Experimental method
[0024] HepG2.2.15 cells in ...
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Abstract
A novel application of pedunculoside in preparation of anti-(hepatitis-B-virus) medicines is disclosed for the first time by the invention. The application includes an application of the pedunculoside in preparation of medicines inhibiting hepatitis B surface antigen secretion or hepatitis B virus E antigen secretion. In-vitro HepG22.2.15 cell pharmacological experiments prove that: the pedunculoside has good anti-HBV functions and has characteristics of a low effective concentration and low cell toxicity; the pedunculoside can inhibit HBsAg and HBeAg; when the concentration is 100 [mu]g/mL, the HBsAg inhibiting rate and the HBeAg inhibiting rate are 34.8% and 35.2% respectively; and an HBV inhibiting function of the pedunculoside is higher than HBV inhibiting functions of positive controls which are lamivudine, oleanolic acid and a ilex rotunda bark extract product.
Description
technical field [0001] The invention relates to a new application of pedicel glucoside. Background technique [0002] Viral infectious diseases have become one of the major diseases that threaten human life and health. Among them, chronic hepatitis B caused by hepatitis B virus (HBV) is a very harmful epidemic disease. According to data released by the World Health Organization (WHO) in March 2015, there are about 240 million chronic HBV infections worldwide, and about 780,000 people die of hepatitis B complications every year. Currently, clinically used anti-HBV drugs mainly include interferon and nucleoside analogues such as lamivudine, entecavir and adefovir. Although they can effectively control the disease, they are expensive, and long-term use has the disadvantages of large side effects, easy drug resistance, and easy rebound after drug withdrawal. [0003] At present, in the process of screening new anti-HBV drugs, many researchers at home and abroad are turning th...
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