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Universal molecular marker primer for tree and joint development method and application thereof

A technology of molecular markers and trees, which is applied in biochemical equipment and methods, microbiological determination/inspection, DNA/RNA fragments, etc., can solve problems such as the inability to extract intron sequences, achieve objective results and improve accuracy

Active Publication Date: 2017-09-22
SHANDONG AGRICULTURAL UNIVERSITY
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Problems solved by technology

[0009] PIP markers are suitable for species that only have published genome sequences but no specific genome structure information. Since there is no detailed intron site information, it is impossible to extract intron sequences according to conventional methods

Method used

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  • Universal molecular marker primer for tree and joint development method and application thereof
  • Universal molecular marker primer for tree and joint development method and application thereof
  • Universal molecular marker primer for tree and joint development method and application thereof

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Embodiment Construction

[0033] The present invention will be specifically introduced below in conjunction with the accompanying drawings and specific embodiments.

[0034] refer to figure 1 , the joint development method of the SSR of tree of the present invention, ILP and PIP universal molecular marker primer, comprises the following steps:

[0035] Step1: SSR site and intron site scanning

[0036] Download all tree genome sequences and genome annotation information from the public data platform, scan the SSR site, intron site and predicted intron site in the genome sequence, and obtain the corresponding site information.

[0037] It is generally believed that the minimum length of an SSR site is 12bp, that is, a single nucleotide repeat unit is at least 12 repeats, a dinucleotide repeat unit is at least 6 repeats, a trinucleotide repeat unit is at least 4 repeats, and a tetranucleotide repeat is at least 4 repeats. The unit is at least 3 repeats, the pentanucleotide repeating unit is at least 3 r...

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Abstract

The invention discloses a universal molecular marker primer for trees and a joint development method and application thereof, wherein the joint development method of three molecular markers is adopted, the limitations of single molecular markers such as the inter-species conservation of the SSR sequences on the two sides is relatively low are circumvented, this greatly limits the use of SSR markers in other species, the ILP markers and IP exist only in the chromosomal gene segment, for other non-coding intervals the method cannot be used, the joint use of multiple molecular marker method combines the advantages of each marker, the result is more objective; the general molecular marker primers SSR, ILP and PIP for trees obtained using the method hereof provide a solution for the problems that currently the molecular markers for trees are relatively few, the universality of the markers is relatively low, and the tree species are difficult to distinguish, and the method is capable of improving the accuracy of the identification results of tree species.

Description

technical field [0001] The present invention relates to molecular marker primers and development methods and applications thereof, in particular to SSR, ILP and PIP universal molecular marker primers of trees, a method for jointly developing the aforementioned universal molecular marker primers based on tree genomes, and the use of the aforementioned universal molecular marker primers in trees The application belongs to the technical field of molecular marker development and application. Background technique [0002] Trees are important natural resources. On the one hand, trees play a huge role in protecting our natural ecological environment. On the other hand, trees are important furniture and industrial raw materials, and have important economic value. [0003] There is a shortage of deep forest resources in my country, uneven distribution between north and south, and low economic benefits. Breeding fine varieties is an effective way to solve the above problems. [0004]...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895C12Q2600/156
Inventor 杨龙桑亚林夏信瑶栾琳琳
Owner SHANDONG AGRICULTURAL UNIVERSITY
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