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Chip and kit for cancer diagnosis

A kit and chip technology, which is applied in the field of medicine and biology, can solve the problems of insufficient sensitivity and difficulty in early screening.

Active Publication Date: 2017-09-29
生工生物工程(上海)股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in gastric cancer screening, traditional methods such as fecal occult blood testing are not sensitive enough, resulting in a large number of false negative results
Therefore, it is still very difficult to conduct early screening for asymptomatic or insignificant patients clinically.

Method used

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  • Chip and kit for cancer diagnosis
  • Chip and kit for cancer diagnosis
  • Chip and kit for cancer diagnosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1 Differential miR analysis in gastric cancer

[0020] 1. Determination of samples

[0021] This experiment selects a large-sample gastric cancer clinical cohort study from the Prince of Wales Hospital in Hong Kong (1998-2002, the average follow-up time is 40.8 months, the longest is 157.9 months), and each case is guaranteed to have more than 5 years of experience. Follow-up data and complete clinical information. This cohort study included 245 cases, all of whom were directly surgically resected without neoadjuvant therapy and were pathologically confirmed as primary gastric cancer. All gastric cancer case information and tissue samples were collected. After deliberation and approval by the Clinical Research Ethics Committee of the Chinese University of Hong Kong, and the informed consent of the patients and their families. Gastric cancer tissues and their paired distal non-tumor gastric tissues were collected during gastric cancer surgery. After collection, t...

Embodiment 2

[0024] Example 2 Effect of Highly Expressed hsa-miR-092a-1 on Cancer Cells

[0025] Gastric cancer cell lines AGS and BGC823 were cultured; the culture medium used was high entanglement DMEM medium (Dulbecco'S modified Eagle's medium, Gibco-Invitrogen, Carlsbad, CA, USA). Cells were placed at 37°C, 5% CO 2 and cultured in a sterile incubator with saturated humidity. Under normal growth conditions, the culture medium was changed every 2-4 days, and the cells were passaged at 1:3 or 1:4 by 0.25% trypsin-EDTA solution (Trypsin-EDTA solution, Gibco-Invitrogen, Carlsbad, CA, USA).

[0026]Construction of an overexpression hsa-miR-092a-1 vector: the miR-092a-1 sequence and its flanking sequences were cloned into the pMD19-T vector, and sequenced by Shanghai Sunny Biotechnology Co., Ltd. The results showed that the sequence of miR-092a-1 was correct. The miR-092a-1 sequence and its flanking sequences confirmed by sequencing were subcloned and inserted between EcoRI / BamHI of the pC...

Embodiment 3

[0037] Example 3 Application of miR-092a-1 in detection of gastric cancer

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Abstract

The invention relates to a micro-nucleotide hsa-miR-092a-1 and a gene chip directed at the micro-nucleotide hsa-miR-092a-1. It is proved in the invention that the molecular marker hsa-miR-092a-1 can be used as a target for targeted improvement of the expression of SCNN1B, can substantially lower expression in the stomach cancer and provides a novel treatment target for effective control and treatment of the stomach cancer.

Description

technical field [0001] The invention relates to a diagnostic chip for gastric cancer and a kit thereof, belonging to the field of medical biotechnology. Background technique [0002] Gastric cancer is one of the most common malignant tumors in the world. In 2012, there were 950,000 new cases of gastric cancer worldwide, and 723,000 deaths, making it the third leading cause of cancer death in the world, and 42.6% of gastric cancers occurred in China. According to the latest research results released by the National Cancer Center of China in 2016, the morbidity and mortality of gastric cancer rank the second among malignant tumors in my country, and it is still the second most common malignant tumor in China. The high incidence and prevalence of gastric cancer pose a significant social burden globally. Especially China, which is still in the developing stage, is currently facing great pressure from cancer. Due to the atypical clinical manifestations of gastric cancer in the...

Claims

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Application Information

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IPC IPC(8): C12N15/113C12Q1/68A61K31/711A61K35/00A61K48/00
CPCA61K31/711C12N15/1135C12N2320/30C12Q1/6886C12Q2600/118C12Q2600/158C12Q2600/178
Inventor 申永智钱云
Owner 生工生物工程(上海)股份有限公司
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