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Compositions and methods for single molecule placement on substrates

A substrate and target molecule technology, applied in the field of molecular biology, can solve the problems of small target area, lack of compatibility, low loading efficiency, etc.

Active Publication Date: 2022-03-22
ILLUMINA INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This approach requires the utilization of high-resolution lithography and results in relatively low loading efficiency due to the small target area
Loading of single nanopores into lipid bilayers is mainly achieved by monitoring the changes in ionic current during the loading process, which lacks compatibility with large-scale production

Method used

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  • Compositions and methods for single molecule placement on substrates
  • Compositions and methods for single molecule placement on substrates
  • Compositions and methods for single molecule placement on substrates

Examples

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Embodiment

[0242] Kinetic exclusion amplification using mismatched capture primer binding regions

[0243] This example shows that mismatched capture primer binding regions prevent recombinase polymerase amplification (RPA) at mismatched target polynucleotide ends.

[0244] In order to confirm that mismatched base pairs between the binding region of the capture primer and the capture primer can prevent the RPA reaction, the commercial kit TwistDX was used in the RPA reaction according to the manufacturer's instructions. Figure 9 Target polynucleotides and capture primers are indicated. Briefly, the target polynucleotide employs an approximately 500 bp phiX target region flanked by capture primer regions termed standard (std) or mismatch (msm). By design, the primer set P5-std / P7-std is complementary to the capture primer binding region in the target polynucleotide phiX-std, and the P5-msm / P7-msm is complementary to the capture primer binding region in the target polynucleotide phiX-msm...

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Abstract

Provided herein are methods and compositions for placing a single target molecule on a patterned substrate.

Description

[0001] This application claims the benefit of US Provisional Patent Application No. 62 / 092,171, filed December 15, 2014, which is hereby incorporated by reference in its entirety for all purposes. [0002] field of invention [0003] The present disclosure relates to the field of molecular biology, and more particularly to compositions and methods for placing single target molecules on patterned substrates. [0004] Background of the invention [0005] Single-molecule placement has useful applications in areas such as biomolecular dynamics studies, drug discovery, and nucleic acid sequencing. For example, single-molecule DNA sequencing has been achieved by placing a single polymerase in a nanometer-sized waveguide. Single-molecule DNA sequencing has been achieved using nanopore structures that load individual nanopore-forming proteins into lipid bilayers. [0006] Currently, single-molecule placement is mainly achieved through molecular size exclusion effects that restrict th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6837B01J19/00
CPCC12Q1/6837C12Q2565/543C12Q1/68B01J2219/00274C12Q2521/543B01J19/0046
Inventor K.L.冈德森J.白B.博亚诺夫
Owner ILLUMINA INC