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Isolation and in-vitro proliferation method of periodontal ligament stem cells

A technology of periodontal ligament stem cells and in vitro proliferation, applied in the field of stem cells, can solve the problems of incomplete and effective regeneration, periodontal tissue defects, etc.

Inactive Publication Date: 2017-10-03
北京泰盛生物科技有限公司
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Problems solved by technology

[0002] Periodontal disease is a chronic infectious disease that occurs in the periodontal tissue. The ultimate goal of its treatment is to achieve the complete regeneration of the periodontal tissue and restore the normal physiological functions of the gingiva, periodontal ligament, alveolar bone and cementum. However, at present, periodontal tissue defects have not been fully and effectively regenerated.

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  • Isolation and in-vitro proliferation method of periodontal ligament stem cells
  • Isolation and in-vitro proliferation method of periodontal ligament stem cells
  • Isolation and in-vitro proliferation method of periodontal ligament stem cells

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Embodiment Construction

[0029] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Apparently, the described embodiments are only some of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0030] refer to figure 1 The method for the isolation and in vitro proliferation of periodontal ligament stem cells is shown. The implementation steps of the method are: firstly select and collect periodontal healthy premolars 1 extracted from patients aged 12-18 due to orthodontic reduction. Dental caries, no defects, sent to the laboratory within 4 hours after extraction; after that, in the ultra-clean workbench 2, rinse the tooth body with PBS solution in a sterile petri d...

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Abstract

The invention discloses an isolation and in-vitro proliferation method of periodontal ligament stem cells. The method is characterized by comprising the following steps: selecting teeth; stripping off periodontal ligaments; centrifuging periodontal tissue blocks; isolating the periodontal ligament stem cells; cultivating the periodontal ligament stem cells; purifying and cloning the periodontal ligament stem cells; proliferating the periodontal ligament stem cells; and the like. The method provided by the invention has the beneficial effects that on the basis of researches on the isolation and in-vitro proliferation method of the periodontal ligament stem cells, a theoretical basis is provided for later researches on construction of biological organs of periodontal tissue engineering and on periodontal regeneration treatment. By isolating and purifying the human periodontal ligament stem cells, biological characteristics can be further recognized, so that an important theoretical basis is offered for later researches on construction of artificial biological organs of a periodontal ligament and cementum complex and on periodontal regeneration treatment.

Description

technical field [0001] The invention relates to the technical field of stem cells, in particular to a method for separating and proliferating periodontal ligament stem cells in vitro. Background technique [0002] Periodontal disease is a chronic infectious disease that occurs in the periodontal tissue. The ultimate goal of its treatment is to achieve the complete regeneration of the periodontal tissue and restore the normal physiological functions of the gingiva, periodontal ligament, alveolar bone and cementum. However, periodontal tissue defects have not been fully and effectively regenerated at present. [0003] Periodontal ligament stem cells belong to adult stem cells. It was first proposed by Byoung in 2004, and it was confirmed that it can form periodontal ligament cementum-like complexes under certain conditions, which will provide a theoretical basis and reliable cells for its tissue engineering research. source. In recent years, the rapid development of this tis...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/074
CPCC12N5/0662C12N2509/00
Inventor 张建国周彦恒施松涛
Owner 北京泰盛生物科技有限公司
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