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Fungus positive blood culture separation gel and chemical reagent pretreatment method

A technology of chemical reagents and separating gels, applied in biochemical equipment and methods, scientific instruments, analytical materials, etc., can solve problems such as time-consuming, save manpower and material resources, and shorten the identification process.

Inactive Publication Date: 2017-10-10
PEKING UNION MEDICAL COLLEGE HOSPITAL CHINESE ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the above problems, the present invention provides a fungal-positive blood culture separation gel and a pretreatment method for chemical reagents, which effectively avoids the time-consuming defect of the entire process in the prior art

Method used

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  • Fungus positive blood culture separation gel and chemical reagent pretreatment method
  • Fungus positive blood culture separation gel and chemical reagent pretreatment method
  • Fungus positive blood culture separation gel and chemical reagent pretreatment method

Examples

Experimental program
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Effect test

Embodiment 1

[0034] The pretreatment method of fungal-positive blood culture separation gel and chemical reagents, the specific steps are as follows:

[0035] Step 1: Draw positive blood culture solution into 8.5ml separation rubber tube;

[0036] Step 2: Put the separation hose into the centrifuge, and the centrifuge centrifuges for 10 minutes under the action of a centrifugal force of 4000g;

[0037] Step 3: Absorb and discard the supernatant of the positive blood culture solution under the condition of not touching the bacterial film on the surface of the separating gel in the separating rubber tube;

[0038] Step 4: Use 1ml of sterile double-distilled water to repeatedly blow and suck the bacterial film on the surface of the separation gel in the separation rubber tube, so as to suspend the bacterial film in sterile double-distilled water to form a bacterial suspension;

[0039] Step 5: Transfer 1ml of the bacterial suspension to a 1.5ml EP tube, put the EP tube into a centrifuge, and...

Embodiment 2

[0057] The pretreatment method of fungal-positive blood culture separation gel and chemical reagents, the specific steps are as follows:

[0058] Step 1: Draw positive blood culture solution into 8.5ml separation rubber tube;

[0059] Step 2: Put the separation hose into the centrifuge, and the centrifuge centrifuges for 10 minutes under the action of a centrifugal force of 4000g;

[0060] Step 3: Absorb and discard the supernatant of the positive blood culture solution under the condition of not touching the bacterial film on the surface of the separating gel in the separating rubber tube;

[0061] Step 4: Use 1ml of sterile double-distilled water to repeatedly blow and suck the bacterial film on the surface of the separation gel in the separation rubber tube, so as to suspend the bacterial film in sterile double-distilled water to form a bacterial suspension;

[0062] Step 5: Transfer 1ml of the bacterial suspension to a 1.5ml EP tube, put the EP tube into a centrifuge, and...

Embodiment 3

[0080] The pretreatment method of fungal-positive blood culture separation gel and chemical reagents, the specific steps are as follows:

[0081] Step 1: Draw positive blood culture solution into 8.5ml separation rubber tube;

[0082] Step 2: Put the separation hose into the centrifuge, and the centrifuge centrifuges for 10 minutes under the action of a centrifugal force of 4000g;

[0083] Step 3: Absorb and discard the supernatant of the positive blood culture solution under the condition of not touching the bacterial film on the surface of the separating gel in the separating rubber tube;

[0084] Step 4: Use 1ml of sterile double-distilled water to repeatedly blow and suck the bacterial film on the surface of the separation gel in the separation rubber tube, so as to suspend the bacterial film in sterile double-distilled water to form a bacterial suspension;

[0085] Step 5: Transfer 1ml of the bacterial suspension to a 1.5ml EP tube, put the EP tube into a centrifuge, and...

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Abstract

A fungal positive blood culture separation gel and chemical reagent pretreatment method, extracting 8.5ml blood culture positive liquid into 8.5ml separation rubber tube; placing the separation rubber tube in a centrifuge, and centrifuging the centrifuge for 10 minutes under the action of a centrifugal force of 4000g; After centrifuging to remove the supernatant, add 1ml Tween Tween20 to the EP tube for repeated suction and blowing, then let the EP tube stand for 3 minutes at room temperature, and centrifuge at 13000rpm for 2 minutes. Do not touch the EP tube. Discard the supernatant under the condition of precipitation at the bottom of the tube; blow and aspirate the precipitate repeatedly with 1ml sterile double distilled water, so as to suspend the bacteria in sterile double distilled water to form a bacterial suspension; the obtained bacterial suspension is treated with chemical reagents Afterwards, direct MALDI-TOF mass spectrometry identification is carried out, which effectively avoids the time-consuming defect of the entire process in the prior art.

Description

technical field [0001] The invention relates to the technical field of fungal positive blood culture, in particular to a fungal positive blood culture separation gel and a pretreatment method for chemical reagents. Background technique [0002] The existing fungal-positive blood culture identification method is: after the blood culture instrument is positive and alarms, it is processed according to the traditional process, smear Gram staining, microscope observation, and planting on a sand-based weak plate, cultured under aerobic conditions for 24-48 hours to obtain pure bacteria If it falls behind, then use existing commercialized instruments based on biochemical reactions or immunological methods such as Vitek 2Compact, API 20C, etc. for identification. [0003] However, the current method needs to transfer the positive blood culture solution to the culture medium, cultivate and incubate until a single pure colony is visible to the naked eye, and then select a single pure ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/04
CPCC12Q1/04G01N2333/195
Inventor 杨启文周梦兰徐英春
Owner PEKING UNION MEDICAL COLLEGE HOSPITAL CHINESE ACAD OF MEDICAL SCI
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