Chimeric antigen receptors (CAR) targeting hematologic malignancies, compositions and methods of use thereof

A chimeric antigen receptor, antibody technology, applied in the direction of antibody mimics/scaffolds, antibody medical components, DNA/RNA fragments, etc.

Active Publication Date: 2017-10-13
ICELL GENE THERAPEUTICS LLC +5
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, such attempts have met with limited success

Method used

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  • Chimeric antigen receptors (CAR) targeting hematologic malignancies, compositions and methods of use thereof
  • Chimeric antigen receptors (CAR) targeting hematologic malignancies, compositions and methods of use thereof
  • Chimeric antigen receptors (CAR) targeting hematologic malignancies, compositions and methods of use thereof

Examples

Experimental program
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example

[0377] result

[0378] Production of the third generation CD5CAR

[0379] Constructs of CD5CAR and anchored CD5 scFv antibodies were designed to test the function and mechanism of CD5CAR T cells to target and lyse CD5 expressing cells and the ability of CD5CAR T cells to downregulate CD5 expression within their own CD5CAR T cell population ( Figure 17A ). To confirm the CD5CAR construct, the resulting CD5CAR lentivirus was transduced into HEK293 cells. After being treated with CD5CAR or GFP-lentivirus for 48 h, the expression of CD5CAR in HEK293 cells was verified by Western blot analysis using CD3ζ antibody, which recognizes the C-terminus of CD5CAR protein ( Figure 17B ). The resulting band was the expected size of CD5CAR protein in CD5CAR-transduced HEK293 cells, but GFP-transduced HEK293 cells did not show any specific bands by western blot analysis. To evaluate the function of CD5CAR protein for subsequent experiments, CD5CAR lentivirus was transduced into activated...

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Abstract

The present disclosure provides chimeric antigen receptor polypeptides having antigen recognition domains for CD2, CD3, CD4, CD5, CD7, CD8, and CD52 antigens, and polynucleotides encoding for the same. The present disclosure also provides for engineered cells expressing the polynucleotide or polypeptides. In some embodiments, the disclosure provides methods for treating diseases associated with CD2, CD3, CD4, CD5, CD7, CD8, and CD52 antigens.

Description

[0001] Cross References to Related Applications [0002] This application is an International PCT application claiming priority to U.S. Provisional Application No. 62 / 121,842. The U.S. Provisional Application was filed on February 27, 2015 and is incorporated herein by reference in its entirety. Background technique [0003] T cells (a type of lymphocyte) play a major role in cell-mediated immunity because of the presence of the T cell receptor (TCR) on the cell surface, which makes it different from other lymphocytes such as B cells and natural killer cells (NK cells). Helper T cells (also known as CD4+ T or CD4 T cells) express the CD4 glycoprotein on their surface. When helper T cells are exposed to peptide antigens presented by MHC class II molecules, the cells are activated. Once activated, these cells proliferate rapidly and secrete cytokines that regulate the immune response. Cytotoxic T cells (also known as CD8+ T cells or CD8 T cells) express the CD8 glycoprotein ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/15A61K39/39A61K48/00A61P35/00C07K14/705
CPCC07K14/7051C07K16/2812C07K16/2803C07K16/2809C07K16/2896C07K16/2806C07K16/2893C12N5/0636C12N5/0646A61K39/001129A61K39/001111A61K35/17A61P19/08A61P1/04A61P3/10A61P31/12A61P37/02A61P9/00A61P17/06A61P25/00A61P29/00A61P43/00A61P17/00A61P17/14A61P35/00A61P1/16A61P13/12A61P15/08A61P21/04A61P27/02A61P7/06A61P35/02A61P37/06A61P7/00A61P19/02A61P27/16A61P29/02C07K2319/02C07K2319/03C07K2319/33C07K2317/622C12N2510/00A61K2039/5158A61K2039/5156C07K2319/00A61K38/00C07K14/70521C07K14/70578A61K2039/505A61K48/005C12N2740/16043C12N15/1138C12N2310/20C12N2501/53C12N2501/515C12N2501/505A61K48/00A61K39/39C07K14/705A61K35/15
Inventor 马玉坡平茨·凯文江迅瓦达·雅之陈凯文
Owner ICELL GENE THERAPEUTICS LLC
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