Preparation method of liposome containing irradiated graphene quantum dots (IGQDs)

A technology of graphene quantum dots and liposomes, which is applied in the direction of graphene, liposome delivery, medical preparations of non-active ingredients, etc., can solve the situation that the drug release cannot be accurately understood drug release effect, drug loading Less problems, to achieve the effect of easy control, low biological toxicity and simple process

Inactive Publication Date: 2017-10-17
SHANGHAI UNIV
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Problems solved by technology

Therefore, long-circulating liposomes are mainly used to carry therapeutic drugs. However, a single long-circulating liposome has the prob

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  • Preparation method of liposome containing irradiated graphene quantum dots (IGQDs)
  • Preparation method of liposome containing irradiated graphene quantum dots (IGQDs)

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[0017] The present invention will be described in further detail below in conjunction with the accompanying drawings and embodiments.

[0018] A method for preparing liposomes containing irradiated graphene quantum dots (IGQDs), the specific steps of the method are as follows:

[0019] (1). Weigh 1.2 g of carbon fiber (CF) into a 250 mL round-bottomed three-necked flask, then add mixed acid and mix, ultrasonicate the mixture for 2 h at room temperature, and then place the ultrasonically mixed solution at 80 °C In an oil bath, heat and reflux for 24 h; after the reaction, cool to room temperature, add 400 mL of deionized water to the mixture, centrifuge at 6000 rpm for 30 min, remove the lower sediment, and collect the upper black solution. Then the black solution is filtered with a filter membrane of 0.22 μm to remove larger particles, and it is dialyzed for 5 days with a 3500D dialysis bag to obtain a graphene quantum dot (GQDs) solution;

[0020] (2). Take 10 mL of the grap...

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Abstract

The invention discloses a preparation method of liposome containing irradiated graphene quantum dots (IGQDs). The method comprises the following steps: 1) weighing carbon fiber (CF) and adding the material in a round-bottom 3-mouth flask, adding the mixed acid for mixing, performing ultrasonic treatment, heating backflow, centrifugation and pumping filtration, and dialysis to obtain a graphene quantum dot (GQDs) solution; 2) weighing the graphene quantum dot (GQDs) solution and adding the material into deionized water and an organic solvent, using electron beams for irradiation, performing dialysis to obtain an irradiated graphene quantum dot (IGQDs) solution; 3) weighing HSPC, CHO, and DSPE-PEG2000 and mixing the materials, adding a mixture in chloroform and methanol for dissolution; weighing the above solution, adding the material into a pear-shaped bottle for rotary evaporation to obtain the liposome, adding the (IGQDs) solution, performing concussion mixing, placing the material in a shaking table for hydration, performing extrusion, and placing the material in a refrigerator; and 4) taking the material out and adding the material in a gel separating column, eluting the material by the deionized water, collecting an eluate to obtain the liposome containing the irradiated graphene quantum dots (IGQDs). The liposome can emit white fluorescence under irradiation of an ultraviolet lamp with 365 nm, and has good water-soluble performance and low biological toxicity.

Description

technical field [0001] The invention relates to a method for preparing liposomes containing irradiated graphene quantum dots (IGQDs). Background technique [0002] Graphene quantum dots (GQDs) are graphene single-layer sheets smaller than 100 nm. Although graphene quantum dots (GQDs) are also a kind of graphene materials, compared with graphene, graphene quantum dots (GQDs) are less With the characteristics of edge effect and quantum effect, these characteristics make it have better development potential than graphene in electronics and optoelectronics, and can be applied to photocatalysts, electrochemical sensors, etc. Moreover, graphene quantum dots (GQDs) also have the characteristics of low toxicity, fluorescence stability, and biocompatibility, and they also have broad development prospects in the fields of drug / gene loading, fluorescent probes, and bioimaging. [0003] Long-circulating liposomes are molecularly ordered assemblies of a multilamellar vesicle structure f...

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Application Information

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IPC IPC(8): A61K9/127A61K47/04A61K49/00C01B32/184B82Y40/00
Inventor 邓小勇朱俊涛杭明光付朝段俊红谢志全郦成徐姣姣常庆
Owner SHANGHAI UNIV
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