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Selenium-rich corn small peptide with liver protection effect and preparation method thereof

A technology for selenium-enriched corn, which is applied in the biological field and achieves the effects of simple preparation, low cost, and small toxic and side effects

Inactive Publication Date: 2017-10-20
ANHUI BIOLOGICAL PEPTIDE IND RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is worth noting that these trace elements must usually be directly or indirectly supplied by the soil, but most people often cannot obtain enough trace elements through diet

Method used

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  • Selenium-rich corn small peptide with liver protection effect and preparation method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Preparation of selenium-enriched corn small peptide: dissolving sodium selenite in deionized water to prepare a solution with a concentration of 5 mg / ml, adding zein at a ratio of 10:1 between the solution and zein, stirring evenly, heating and boiling for 15 minutes , after cooling to about 45°C, adjust the pH value to 2.0 with dilute hydrochloric acid, add 1.5% pepsin, keep warm for 2 hours, adjust the pH value to 8.0, add 1.0% trypsin, keep warm for 4 hours, boil for 15 minutes, centrifuge, and discard the precipitate , the supernatant was ultrafiltered through an ultrafiltration membrane with a relative molecular mass of 3KD, the permeate was added to the DA201-C macroporous adsorption resin column, eluted with water, the washing solution was discarded, and then washed with 75% (V / V ) ethanol elution, collect the ethanol eluate, recover ethanol under reduced pressure and continue to concentrate to a relative density of 1.12 (60°C), and spray dry (inlet air temperatur...

Embodiment 2

[0037]Preparation of selenium-enriched corn small peptide: dissolving sodium selenite in deionized water to prepare a solution with a concentration of 4 mg / ml, adding zein at a ratio of 8:1 between the solution and zein, stirring evenly, heating and boiling for 10 minutes , after cooling to about 50°C, adjust the pH value to 3.0 with dilute hydrochloric acid, add 2% pepsin, keep warm for 3 hours, adjust the pH value to 7.5, add 2% trypsin, keep warm for 5 hours, boil for 10 minutes, centrifuge, and discard the precipitate , the supernatant was ultrafiltered through an ultrafiltration membrane with a relative molecular mass of 3KD, the permeate was added to the DA201-C macroporous adsorption resin column, eluted with water, the washing solution was discarded, and then 60% (V / V ) ethanol elution, collect the ethanol eluate, recover ethanol under reduced pressure and continue to concentrate to a relative density of 1.15 (60°C), spray dry (inlet air temperature is 180-200°C, outlet...

Embodiment 3

[0039] Get the selenium-enriched corn small peptide 100g that the method for Example 1 gains, by selenium-enriched corn small peptide: corn oil: beeswax: lecithin ratio is 10:35:4:1 mixed material, through grinding 30 minutes, with gelatin, purified water , glycerin, and iron oxide black in the ratio of 100:100:40:1 to make soft capsule skin glue, control the temperature of the plastic box at 40-50°C, the temperature of the heat preservation tank at 35-45°C, and the temperature of the spray body at 30-40°C, and press it into a soft capsule. Capsules, the content of each capsule weighs 0.45g, dried in cold air at 17°C-27°C for 3-5h to finalize the shape, washed pills, dried, selected pills, packed, and prepared into finished soft capsules.

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Abstract

Belonging to the field of biotechnologies, the invention discloses a selenium-rich corn small peptide with liver protection effect and a preparation method thereof. The method is characterized by: taking corn protein as the starting material, and conducting chelate enzymolysis, ultrafiltration, resin column purification, decompression concentration and spray drying, thus obtaining the selenium-rich corn small peptide. The selenium-rich corn small peptide can be takes as the main raw material, and is added with other raw materials and auxiliary materials to be prepared into some corresponding preparations according to conventional preparation methods. The method adopted by the invention carries out enzymolysis and trace element enrichment synchronously, has the characteristics of high trace element enrichment efficiency and high element organification rate, and the prepared selenium-rich corn small peptide has significant liver protection effect.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a selenium-enriched corn small peptide with hepatoprotective effect and a preparation method thereof. Background technique [0002] The liver is the largest metabolic organ in the human body. All the food we eat needs to be synthesized and decomposed by the liver. But the liver is also an organ that is prone to "disease", and daily maintenance is very important. The liver is the "chemical factory" of the human body. There are more than 1,500 main physiological functions of the liver. It is the "chemical factory" of the human body, which synthesizes proteins, enzymes and various substances needed by the body. The liver is also the largest digestive organ in the human body, and it secretes bile through liver cells to help the digestive function of the human body. The liver also has a reserve function, storing energy and substances necessary for the human body such as vitamins, fats,...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/34C07K1/16A61K38/01A61P1/16
CPCA61K38/011C07K1/16C07K1/34C12P21/06
Inventor 刘国飞刘玉军柏道鸣
Owner ANHUI BIOLOGICAL PEPTIDE IND RES INST
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