Method for analyzing effective components of astragali radix and poria preparation with effects of warming spleen and eliminating cysts

An analysis method and technology of active ingredients, applied in the field of analysis of active ingredients of Qiling Wenshen Xiaoyao preparation

Active Publication Date: 2017-10-20
TIANJIN TASLY PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there are few reports on the material basis of Qiling Wenshen Xiaonang Decoction

Method used

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  • Method for analyzing effective components of astragali radix and poria preparation with effects of warming spleen and eliminating cysts
  • Method for analyzing effective components of astragali radix and poria preparation with effects of warming spleen and eliminating cysts
  • Method for analyzing effective components of astragali radix and poria preparation with effects of warming spleen and eliminating cysts

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0098] Preparation of reference solution

[0099] Weigh an appropriate amount of chlorogenic acid, acteosin glucoside, acteosin, hyperoside, rosmarinic acid, shikonic acid, formononetin, salvianolic acid A, icariin, formononetin, astragalus Add glycoside A, baojoside I, cucuroside B, atractyloside A, chaohuoding A, chaohuoding B, and chaohuoding C reference substances in the same measuring bottle, add an appropriate amount of methanol for ultrasonic dissolution, cool to room temperature, and set When the volume reaches the mark, the mixed reference solution is obtained.

[0100] Preparation of the test solution

[0101] Accurately weigh 0.5 g of the extract of Qiling Wenshen Xiaonang Formula, place it in a stoppered Erlenmeyer flask, accurately add 10 mL of 30-50% methanol, weigh the mass, ultrasonically (power 100W, frequency 40kHz) for 20-60min, cool Bring to room temperature, make up for the lost weight, shake well, centrifuge, take the supernatant and pass through a 0.22...

Embodiment 2

[0107] Proceed as follows:

[0108] (1) Preparation of the control solution: Weigh an appropriate amount of chlorogenic acid, acteosin glucoside, actcoisoflavone, hyperoside, rosmarinic acid, shikonic acid, formononetin, salvianolic acid A, epimedium Glycoside, formononetin, astragaloside IV, baohuoside I, cuculoside B, atractyloside A, Chaohuoding A, Chaohuoding B, Chaohuoding C reference substances in the same measuring bottle, add 40% Methanol was ultrasonically dissolved, cooled to room temperature, and the volume was adjusted to the mark to obtain the mixed reference solution. In the obtained solution, the concentrations of various components are as follows: 0.062, 0.057, 0.0106, 0.047, 0.063, 0.0195, 0.02, 0.059, 0.038, 0.0201, 0.0238, 0.0164, 0.0354, 0.0182, 0.075, 0.09, 0.084mg / ml

[0109] (2) Preparation of the test solution:

[0110] Accurately weigh 0.5028g of Qiling Wenshen Xiaoyao preparation, crush it, add 40% methanol, ultrasonicate for 20-60min, cool to room ...

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PUM

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Abstract

The invention relates to a method for analyzing effective components of a drug, in particular to a method for analyzing effective components of an astragali radix and poria preparation with effects of warming the spleen and eliminating cysts. The method comprises the steps as follows: (1) preparation of a control solution; (2) preparation of a test article solution; (3) analysis method of the effective components including flavonoids, phenolic acids, triterpenoid saponins, sesquiterpene glycosides and alkaloids.

Description

Technical field: [0001] The invention relates to a method for analyzing the effective components of medicines, in particular to a method for analyzing the effective components of Qiling Wenshen Xiaoyao preparation. Background technique: [0002] Polycystic ovary syndrome (PCOS) is a common gynecological disease in adolescents and women of childbearing age, and the incidence rate in women of childbearing age is about 6% to 10%. Traditional Chinese medicine believes that the pathogenesis of PCOS is "disorder of Tiangui, phlegm obstructing the uterus", which is related to the dysfunction of spleen and kidney. Modern medical research has found that PCOS is due to abnormal regulation of the hypothalamus-pituitary-ovary axis, which leads to endocrine disorders, and is characterized by hyperandrogenemia, insulin resistance and ovulation disorders. of the tetralogy. [0003] Qiling Wenshen Xiaosang Recipe (formerly known as Bushen Huatan Recipe) is a prescription with many years o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02G01N2030/027
Inventor 王玉张晓川张依倩崔志鹏何毅
Owner TIANJIN TASLY PHARMA CO LTD
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