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Construction method and culture solution of double-layer skin model for cosmetic anti-aging detection

A technology of skin model and culture solution, applied in biochemical equipment and methods, culture process, tissue culture, etc., can solve the problems of easy shrinkage of dermis, loose connection of dermis and epidermis, affecting scientificity and accuracy, etc., and achieve reduction of dermis Shrinkage, prevent detachment, and strengthen the effect of tissue-engineered double-layer skin dermal-epidermal junction

Active Publication Date: 2021-03-19
GUANGDONG BOXI BIO TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although the current research on tissue-engineered skin has made positive progress, there are still many problems and confusions, such as (1) the dermis is easy to shrink during the construction of the double-layer skin model (2) the connection between the dermis and the epidermis is not tight, etc., which seriously affects It is scientific and accurate when used in cosmetic testing

Method used

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  • Construction method and culture solution of double-layer skin model for cosmetic anti-aging detection
  • Construction method and culture solution of double-layer skin model for cosmetic anti-aging detection
  • Construction method and culture solution of double-layer skin model for cosmetic anti-aging detection

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Embodiment 1

[0043] The construction method of the double-layer skin model used for cosmetics anti-aging detection in this embodiment specifically includes the following steps:

[0044] Step 1, preparation of collagen solution: dissolving collagen in sterilized acetic acid solution, and standing overnight at 4° C. to obtain collagen acetic acid solution; Neonatal bovine serum: DEME (10×) = 1:1:8 volume ratio and mix evenly, then adjust the pH value to 7.2 with 1M NaOH solution to obtain a collagen solution; the volume concentration of the acetic acid solution is 0.1%, and the collagen acetic acid solution The concentration of collagen in the medium is 6g / L;

[0045] Step 2. Preparation of cell-collagen mixture: add fibroblasts to the collagen solution described in step 1 at a density of 7×105 cells / mL to obtain cell-collagen mixture I; add fibroblasts at a density of 2×105 cells / mL In the collagen solution described in step 1, cell-collagen mixture II is obtained;

[0046] The fibroblast...

Embodiment 2

[0052] The construction method of the double-layer skin model used for cosmetics anti-aging detection in this embodiment specifically includes the following steps:

[0053]Step 1, preparation of collagen solution: dissolving collagen in sterilized acetic acid solution, and standing overnight at 4° C. to obtain collagen acetic acid solution; Neonatal bovine serum: DEME (10×) = 7:2:1 volume ratio mixed evenly, then adjust the pH value to 7.4 with 1M NaOH solution to obtain a collagen solution; the mass concentration of the acetic acid solution is 0.1%, the collagen acetic acid solution The concentration of collagen in the medium is 4g / L;

[0054] Step 2. Preparation of cell-collagen mixture: add fibroblasts to the collagen solution described in step 1 at a density of 9×105 cells / mL to obtain cell-collagen mixture I; add fibroblasts at a density of 3×105 cells / mL In the collagen solution described in step 1, cell-collagen mixture II is obtained;

[0055] The fibroblasts are the...

Embodiment 3

[0061] The construction method of the double-layer skin model used for cosmetics anti-aging detection in this embodiment specifically includes the following steps:

[0062] Step 1, preparation of collagen solution: dissolving collagen in sterilized acetic acid solution, and standing overnight at 4° C. to obtain collagen acetic acid solution; Neonatal bovine serum: DEME (10×) = 8:1:1 volume ratio mixed evenly, then adjust the pH value to 7.3 with 1M NaOH solution to obtain a collagen solution; the concentration of the acetic acid solution is 0.1%, the collagen acetic acid solution The concentration of collagen is 8g / L;

[0063] Step 2. Preparation of cell-collagen mixture: add fibroblasts to the collagen solution described in step 1 at a density of 6×105 cells / mL to obtain cell-collagen mixture I; add fibroblasts at a density of 2.5×105 cells / mL In the collagen solution described in step 1, cell-collagen mixture II is obtained;

[0064] The fibroblasts are the 6th generation ...

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Abstract

The invention discloses a construction method of a double-layer skin model for anti-aging detection of cosmetics and a culture solution. The culture solution contains a culture solution A, a culture solution B, a culture solution C and a culture solution D, wherein the culture solution A is prepared by adding NaHCO3, progesterone, butanediamine, sodium selenate, insulin, fetal calf serum, hydrocortisone, adenine, a base fibroblast growth factor, transferrin, an angiogenesis factor and an epidermal growth factor into a base solution, namely a mixed solution of a DMEM culture solution and an F12 culture solution; and the culture solution B, the culture solution C and the culture solution D contain the culture solution A, vitamin C, vitamin E, calcium chloride, C-xyloside and magnesium aspartate. Besides, the invention further discloses a method for constructing the double-layer skin model for the anti-aging detection of cosmetics by virtue of the culture solution. By utilizing the culture solution, the linkage of dermis and epidermis of double-layer skin in tissue engineering can be strengthened.

Description

technical field [0001] The invention belongs to the technical field of tissue engineering, and in particular relates to a construction method and a culture solution of a double-layer skin model for cosmetic anti-aging detection. Background technique [0002] As the largest organ of the human body, the skin has powerful functions and is the first physiological barrier of the human body, which can protect various tissues and organs in the body from physical, mechanical, chemical and pathogenic microorganisms. It is also the organ most affected by the external environment. In recent years, with the aggravation of air pollution, ultraviolet rays are more likely to penetrate the atmosphere and act on human skin, aggravating the photoaging of exposed skin. Therefore anti-aging cosmetics are more and more concerned and favored by people all over the world, and cosmetics with anti-aging effects aimed at skin have emerged in large numbers in the cosmetics market at home and abroad. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/071C12N5/077
CPCC12N5/0625C12N5/0656C12N5/0698C12N2500/12C12N2500/14C12N2500/16C12N2500/25C12N2500/32C12N2500/34C12N2500/38C12N2500/40C12N2500/46C12N2500/84C12N2501/11C12N2501/115C12N2501/165C12N2533/54
Inventor 李潇卢永波
Owner GUANGDONG BOXI BIO TECH CO LTD
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