Preparation method and application of degreased sterilized profound-hypothermia allogeneic bone

Abstract

The invention relates to an implanted biological material medical device and specifically relates to a preparation method and application of degreased sterilized profound-hypothermia allogeneic bone. The preparation method of the degreased sterilized profound-hypothermia allogeneic bone is characterized by comprising the following steps: utilizing human allogeneic bone as a raw material; cutting into bone sections, bone pieces and bone particles; cleaning and removing marrow; degreasing under a temperature of 20 to 40 DEG C; soaking by ethyl alcohol and cleaning by purified water; then packaging; storing the packaged product in an environment below -40 DEG C for at least 12 hours; then putting into a thermal-insulation container; putting ice cake s around the thermal-insulation container; packaging the container and performing irradiation sterilization by cobalt-60 with the dosage of 20 to 25kGy; obtaining the degreased sterilized profound-hypothermia allogeneic bone and storing the degreased sterilized profound-hypothermia allogeneic bone in an environment below -40 DEG C. The degreased sterilized profound-hypothermia allogeneic bone disclosed by the invention has the characteristics of applicability to bone defect bone graft repair, good biocompatibility and mechanical strength, small immunological rejection, high safety, union with graft field and gradual substitution in receptor self bone tissue.

Description

technical field [0001] The invention relates to an implantable biomaterial medical device, in particular to a preparation method and application of degreasing and sterilizing deep-low temperature allogeneic bone. Background technique [0002] Diseases such as tumors and fractures can lead to bone defects and structural damage. In the prior art, autologous bone, metal, polymer materials and ceramics are used to repair bone defects. The amount of autologous bone is limited, and the material is often cancellous bone. The mechanical strength cannot meet the requirements, and the shape is difficult to be consistent with the affected area, which increases the trauma and complications of the patient. Metals, polymer materials, and ceramics have poor biocompatibility, cannot form new bone or have poor bone formation ability, and it is difficult to heal with the bone in the recipient area, and the shape is often difficult to match the recipient area. There are also deep-low temperat...

AI Technical Summary

Problems solved by technology

Metals, polymer materials, and ceramics have poor biocompatibility, cannot form new bone or have poor bone formation ability, and it is difficult to heal with the bone in the recipient area, and the shape is often difficult to match the recipient area

There are also deep-low temperature allografts used to repair bone defects, but this kind of deep-low temperature allogeneic bone is only cryopreserved after cutting, without bone marrow removal, degreasing, alcohol immersion, purified water cleaning, and irradiation in a low-temperature environment. In this way, the symptoms of immune rejection of allograft bone are relatively obvious, the bone formation ability is poor, and the risk of wound infection and disease transmission is also increased.