Rapid Propagation Method of Ethnic Spice Plant Hemp Root
A spice plant, a fast technology, applied in the biological field, can solve the problems of adult plant damage, no hemp root reproduction, etc., and achieve the effect of strong operability and rapid expansion of the seedling base
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[0018] Rapid propagation of plant hemp root (P.magen B.Q.Cheng ex C.L.long&J.Yang)):
[0019] Including: establishment of sterile clones, optimal selection of medium, screening to promote the formation of cluster buds and rooting culture, among which: the medium for inducing axillary buds from stem segments is ①1 / 4MS+BA0.02mg / L+NAA0.05mg / L ; The medium for inducing the proliferation of young stem cluster buds is ②1 / 4MS+BA0.2mg / L+IAA0.1mg / L and ③1 / 4MS+BA0.5mg / L; the medium for inducing rooting is ④1 / 4MS+IAA1mg / L+ PP3330.1mg / L
[0020] Breeding method:
[0021] 1. Establishment of sterile clones:
[0022] Material source: Plant hemp root (P.magen B.Q.Cheng ex C.L.long&J.Yang) is collected from the limestone wall at an altitude of about 1500m in Xishuangbanna area in southern Yunnan.
[0023] The establishment of the sterile line is firstly the disinfection and sterilization of the explants. The sterilization of the explant materials is an important link in the tissue culture,...
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